RESUMO
The transcription factor p63 is required for proper epidermal barrier formation and maintenance. Herein, we used chromatin immunoprecipitation coupled with DNA sequencing to identify novel p63 target genes involved in normal human epidermal keratinocyte (NHEKs) growth and differentiation. We identified over 2000 genomic sites bound by p63, of which 82 were also transcriptionally regulated by p63 in NHEKs. Through the discovery of interleukin-1-α as a p63 target gene, we identified that p63 is a regulator of epithelial-mesenchymal crosstalk. Further, three-dimensional organotypic co-cultures revealed TCF7L1, another novel p63 target gene, as a regulator of epidermal proliferation and differentiation, providing a mechanism by which p63 maintains the proliferative potential of basal epidermal cells. The discovery of new target genes links p63 to diverse signaling pathways required for epidermal development, including regulation of paracrine signaling to proliferative potential. Further mechanistic insight into p63 regulation of epidermal cell growth and differentiation is provided by the identification of a number of novel p63 target genes in this study.
Assuntos
Queratinócitos/metabolismo , Comunicação Parácrina , Transativadores/metabolismo , Proteínas Supressoras de Tumor/metabolismo , Sítios de Ligação , Diferenciação Celular , Linhagem Celular , Imunoprecipitação da Cromatina , Regulação da Expressão Gênica , Humanos , Interleucina-1alfa/genética , Interleucina-1alfa/metabolismo , Queratinócitos/citologia , Interferência de RNA , RNA Interferente Pequeno/metabolismo , Transativadores/genética , Proteína 1 Semelhante ao Fator 7 de Transcrição/genética , Proteína 1 Semelhante ao Fator 7 de Transcrição/metabolismo , Fatores de Transcrição , Proteínas Supressoras de Tumor/genéticaRESUMO
Blood samples were collected from 108 wild hogs (Sus scrofa) from the Great Smoky Mountains National Park (GSMNP), USA, February to July 1990. We found no antibodies for swine brucellosis, pseudorabies, bovine virus diarrhea virus or porcine rotavirus infection. Antibody titers to porcine parvovirus were found in 15 (14%) samples and antibody to one or more leptospiral serovars was found in 48 (44%) samples. Thirty-nine (89%) of the 44 positive samples reacted to all five leptospiral serovars tested.
Assuntos
Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Brucelose/veterinária , Pseudorraiva/epidemiologia , Doenças dos Suínos/epidemiologia , Animais , Animais Selvagens , Brucella/imunologia , Brucelose/epidemiologia , Vírus da Diarreia Viral Bovina/imunologia , Feminino , Herpesvirus Suídeo 1/imunologia , Leptospira interrogans/imunologia , Leptospirose/epidemiologia , Leptospirose/veterinária , Masculino , Parvoviridae/imunologia , Infecções por Parvoviridae/epidemiologia , Infecções por Parvoviridae/veterinária , Prevalência , Rotavirus/imunologia , SuínosRESUMO
A buffered Brucella plate antigen, pH 4.0, was developed for testing bovine and porcine sera which markedly reduced the number of "nonspecific" plate agglutination test reactions. Use of this antigen in a presumptive test decreased testing costs by lowering the amount of "nonspecific" reaction obtained on the standard plate agglutination test and reducing the use of the card test. The test is slightly more sensitive than the card test. Specificity and sensitivity were evaluated using sera collected from experimentally vaccinated and/or infected animals and from animals at slaughter. The antigen is currently being used in a presumptive test for brucellosis in the United States.
