[The stability of all-trans retinoic acid in "retinoid solutions" of "Di Bella's Cure"]. / Stabilità dell'acido all-trans retinoico nella "soluzione ai retinoidi" della "cura Di Bella".

Recently all-trans retinoic acid, an oxidation product of retinol, has become famous, since it is a component of "retinoids solution" of "Di Bella's therapy". Since all-trans retinoic acid is rapidly destroyed in the presence of light and oxidants, we verified its stability in samples of "retinoids solution" stored in conditions believed optimal (under stream of nitrogen) and we compare the obtained results with those observed in other "retinoids solution" samples daily open and close again, simulating the intake from the patient. All samples showed a decrease of all-trans retinoic acid recovery at the end of the study, with a more rapid decline in samples daily open. From our observations, we decided to indicate an expiration date of one months from the date of "solution" preparation. The patient's manipulation brings out a change in the final composition of "retinoids solution".

Clinical pharmacokinetics of tacrolimus in heart transplant recipients.

We report pharmacokinetic data on tacrolimus in 14 heart transplant patients (2 women, 12 men). The median age and the median body weight were 55.5 years (range, 23-61 years) and 67.0 kg (55-79 kg), respectively. All patients were maintained on a triple-drug protocol (tacrolimus, azathioprine, and prednisone), with a 7-day antithymocyte globuline induction. The first tacrolimus dose, administered orally 1 to 5 days posttransplant, ranged from 0.03 to 0.4 mg/kg (median = 0.052 mg/kg). The maintenance dose ranged from 0.03 to 0.13 mg/kg/day (administered in two equal doses) to achieve blood levels of 5 of 20 ng/ml, as determined by a microparticle enzyme immunoassay (MEIA). Whole blood samples were drawn just before, at 0.5 hour, and at 1, 2, 3, 4, 6, 8, 10, and 12 hours after the administration of the first dose; trough levels were measured thereafter. The mean oral clearance (CL/F) and apparent volume of distribution (Vd/F) averaged 0.21+/-0.08 L/hour/kg and 2.4+/-0.8 L/kg while the half-life averaged 8.7+/-3.5 hours. Tacrolimus accumulation index during chronic therapy (Rac = Cmin(steady state)/Cmin(first dose) normalized to the same dose) averaged 1.3. Eighty-eight percent of the trough blood levels measured in our patients were within 5 and 20 ng/ml. The incidence of rejection in the study population was extremely low: a prevalence of grade 2 rejection or more, of 0.4+/-0.8 episodes/ patient was observed after a follow-up period of 8.8+/-2.2 months. Only one patient experienced severe renal toxicity, probably because of his preoperative precarious hemodynamic status. Pharmacokinetic data suggest that maintenance tacrolimus daily dose should be equal to 0.1 mg/kg/day to obtain trough blood concentrations of approximately 10 ng/ml. Inter- and intra-patient variability of tacrolimus blood concentration should be expected and justify careful monitoring.