RESUMO
Restriction fragment length polymorphisms (RFLPs) of C kappa, V kappa II, V lambda VII and VHIII genes were studied from a pool of 104 subjects with rheumatoid arthritis and 96 controls. There were no overall associations with either rheumatoid arthritis itself, or with HLA-DR4-positive and DR4-negative RA subgroups within rheumatoid subjects in this UK Caucasoid population.
Assuntos
Artrite Reumatoide/genética , Genes de Imunoglobulinas/genética , Região Variável de Imunoglobulina/genética , Cadeias kappa de Imunoglobulina/genética , Cadeias lambda de Imunoglobulina/genética , Polimorfismo Genético/genética , Alelos , Artrite Reumatoide/imunologia , Humanos , Polimorfismo de Fragmento de RestriçãoRESUMO
Genetic variants at DRB1 (Dw subtypes), DQB, and C4 loci were compared in rheumatoid disease subjects with or without the extra-articular feature of Felty's syndrome or major vasculitis. DR4 positive subjects with rheumatoid arthritis alone showed no preferential associations with DQB or Dw variants or with C4 null alleles. Felty's subjects showed associations with the DQB encoded DQw7 allele and with the C4B null allele but no preferential associations with any Dw subtype of DR4. By contrast DR4 +ve rheumatoid-vasculitic subjects showed associations with the Dw14 as well as with DQw7 and the C4A null allele. These different MHC associations in different clinical disease subsets show that rheumatoid disease is immunogenetically heterogeneous and suggest that MHC genes outside the DRB1 locus may also influence susceptibility or modify expression of the rheumatoid disease process.
Assuntos
Artrite Reumatoide/imunologia , Complemento C4/análise , Antígenos HLA-D/análise , Antígenos HLA-DQ/análise , Complexo Principal de Histocompatibilidade/imunologia , Doenças Reumáticas/imunologia , Alelos , Sequência de Aminoácidos , Artrite Reumatoide/genética , Síndrome de Felty/genética , Síndrome de Felty/imunologia , Antígeno HLA-DR4/análise , Haplótipos , Humanos , Complexo Principal de Histocompatibilidade/genética , Dados de Sequência Molecular , Doenças Reumáticas/genética , Vasculite/genética , Vasculite/imunologiaRESUMO
DQ beta and C4 null alleles have been defined in patients with rheumatoid arthritis, Felty's syndrome, and in control subjects. Comparison of DR4 positive subjects shows that rheumatoid disease without extra-articular features has no preferential associations with either DQ beta or C4 null variants. In Felty's syndrome there are significant associations with both the class II major histocompatibility complex (MHC) DQw7 allele (86% of DR4 positive patients with Felty's syndrome and 53% of DR4 positive controls) and the class III MHC C4B null allele (50% of patients with Felty's syndrome and 20% of DR4 positive controls). DQw7 and the C4B null allele are in linkage disequilibrium and the B44-Bf *S-C4A*3-C4B*Q0-DR4-DQw7 haplotype accounts for five of 24 DR4 positive haplotypes assigned in subjects with Felty's syndrome. The results were not accounted for by articular disease severity and suggest that articular and extra-articular forms of rheumatoid disease may be immunogenetically heterogeneous.
Assuntos
Artrite Reumatoide/genética , Complemento C4b/genética , Síndrome de Felty/genética , Antígenos HLA-DQ/genética , Alelos , Artrite Reumatoide/imunologia , Marcadores Genéticos , Antígeno HLA-DR4/análise , Haplótipos/genética , Humanos , Desequilíbrio de Ligação/genéticaRESUMO
Restriction fragment length polymorphisms (RFLPs) of alpha-1-antitrypsin were studied in 99 subjects with rheumatoid arthritis alone, 21 subjects with rheumatoid arthritis and pulmonary fibrosis, 26 subjects with rheumatoid arthritis and bronchiectasis, and 86 controls. No associations with either rheumatoid arthritis itself or with the associated pulmonary disorders were noted in this U.K. Caucasoid population.
Assuntos
Artrite Reumatoide/genética , Bronquiectasia/genética , Polimorfismo de Fragmento de Restrição , Fibrose Pulmonar/genética , alfa 1-Antitripsina/genética , Alelos , Artrite Reumatoide/complicações , Bronquiectasia/etiologia , Predisposição Genética para Doença , Humanos , Fenótipo , Fibrose Pulmonar/etiologia , Deficiência de alfa 1-AntitripsinaRESUMO
DQ A and DX A RFLPs were studied in DR4-positive rheumatoid arthritis, Felty's syndrome, and in DR4-positive control subjects, and in the light of the previously documented association between Felty's syndrome and the DQ B 3.1 allele (3b RFLP). In these DR4-positive subjects there were no preferential associations between DQ A or DX A polymorphisms and rheumatoid arthritis or Felty's syndrome and no evidence for unusual DQ A-B haplotypic associations in Felty's or rheumatoid subjects.
Assuntos
Artrite Reumatoide/imunologia , Síndrome de Felty/imunologia , Antígenos HLA-D/genética , Antígenos HLA-DQ/genética , Polimorfismo de Fragmento de Restrição , Variação Genética , Antígeno HLA-DR4/análise , Haplótipos , HumanosRESUMO
Epithelial cells from enzymically dispersed seminal vesicle tissue of castrated adult rats were grown on collagen-coated surfaces in serum-containing medium for up to 6 weeks. Androgens were neither required for growth nor were they mitogenic. They also failed to induce the synthesis of tissue-specific secretory proteins that serve as markers of androgen action in vivo. A variety of potential growth factors and culture procedures (such as feeder layers, extracellular matrices, collagen gels, and growth from tissue explants) were examined for their ability to support androgen action. Testosterone and 5 alpha-dihydrotestosterone were only able to induce seminal vesicle secretory protein synthesis in the presence of neurotransmitters (acetylcholine, noradrenaline, or serotonin). The mechanism of this permissive effect of neurotransmitters on androgenic induction has been examined. It is not related to their mitogenic activity since other mitogens (epidermal growth factor and corticosterone) did not facilitate androgen action. Agents that raise intracellular cAMP were also ineffective so cAMP is probably not involved, but the phosphatidyl inositol pathway may be important.
