Sumatriptan alleviates nitroglycerin-induced mechanical and thermal allodynia in mice.

The association between the clinical use of nitroglycerin (NTG) and headache has led to the examination of NTG as a model trigger for migraine and related headache disorders, both in humans and laboratory animals. In this study in mice, we hypothesized that NTG could trigger behavioural and physiological responses that resemble a common manifestation of migraine in humans. We report that animals exhibit a dose-dependent and prolonged NTG-induced thermal and mechanical allodynia, starting 30-60 min after intraperitoneal injection of NTG at 5-10 mg/kg. NTG administration also induced Fos expression, an anatomical marker of neuronal activity in neurons of the trigeminal nucleus caudalis and cervical spinal cord dorsal horn, suggesting that enhanced nociceptive processing within the spinal cord contributes to the increased nociceptive behaviour. Moreover, sumatriptan, a drug with relative specificity for migraine, alleviated the NTG-induced allodynia. We also tested whether NTG reduces the threshold for cortical spreading depression (CSD), an event considered to be the physiological substrate of the migraine aura. We found that the threshold of CSD was unaffected by NTG, suggesting that NTG stimulates migraine mechanisms that are independent of the regulation of cortical excitability.

Narrative discourse in children with early focal brain injury.

Children with early brain damage, unlike adult stroke victims, often go on to develop nearly normal language. However, the route and extent of their linguistic development are still unclear, as is the relationship between lesion site and patterns of delay and recovery. Here we address these questions by examining narratives from children with early brain damage. Thirty children (ages 3:7-10:10) with pre- or perinatal unilateral focal brain damage and their matched controls participated in a storytelling task. Analyses focused on linguistic proficiency and narrative competence. Overall, children with brain damage scored significantly lower than their age-matched controls on both linguistic (morphological and syntactic) indices and those targeting broader narrative qualities. Rather than indicating that children with brain damage fully catch up, these data suggest that deficits in linguistic abilities reassert themselves as children face new linguistic challenges. Interestingly, after age 5, site of lesion does not appear to be a significant factor and the delays we have witnessed do not map onto the lesion profiles observed in adults with analogous brain injuries.

Genetic analysis of punt, a type II Dpp receptor that functions throughout the Drosophila melanogaster life cycle.

TGF-beta (transforming growth factor-beta-) mediated signal transduction affects growth and patterning in a variety of organisms. Here we report a genetic characterization of the Drosophila punt gene that encodes a type II serine/threonine kinase TGF-beta/Dpp (Decapentaplegic) receptor. Although the punt gene was originally identified based on its requirement for embryonic dorsal closure, we have documented multiple periods of punt activity throughout the Drosophila life cycle. We demonstrate that potentially related embryonic punt phenotypes, defects in dorsoventral patterning and dorsal closure, correspond to distinct maternal and zygotic requirements for punt. In addition, we document postembryonic requirements for punt activity. The tight correspondence between both embryonic and postembryonic loss-of-function punt and dpp phenotypes implicates a role for Punt in mediating virtually all Dpp signaling events in Drosophila. Finally, our comparison of punt homoallelic and heteroallelic phenotypes provides direct evidence for interallelic complementation. Taken together, these results suggest that the Punt protein functions as a dimer or higher order multimer throughout the Drosophila life cycle.

Processing across the language boundary: a cross-modal priming study of Spanish-English bilinguals.

A cross-modal naming paradigm was used to assess within- and between-language sentential priming in Spanish-English bilinguals. The paradigm used single-language auditory texts with visual target words under normal, visually degraded, speeded, and delayed naming conditions. Cross-language priming was always observed when the target language was predictable (in the blocked condition), even under speeded conditions. When the target language was unpredictable (in the mixed condition), cross-language priming was observed only when response was delayed (delayed naming) and under a subset of conditions when word recognition was delayed (visual degradation). Results are compatible with the idea that cross-language priming in a sentence context is more likely to involve the use of expectations, strategic processes, or both that allow bilinguals to tune themselves to external conditions. There are enough exceptions to the general rule, however, to warrant a reconsideration of the lexical-postlexical dichotomy. Implication for modular versus interactive models of lexical access are discussed.

On the preservation of word order in aphasia: cross-linguistic evidence.

Studies of language production in English-speaking aphasics (both fluent and nonfluent) generally lead to the conclusion that word order is preserved to a much greater degree than grammatical morphology and/or lexical retrieval. However, because word order is rigidly preserved even in normal English speech, this pattern might reflect nothing more than "the weak link in the chain." Using a constrained production paradigm, we provide evidence showing that canonical sentence order is well preserved in both fluent and nonfluent patients, in Italian and German (languages that permit much more pragmatic word-order variation) as well as English. Patients also retain the ability to order nouns around a preposition, and among Italian patients, access to a high-frequency form of pragmatic word-order variation is also retained. Syntactic difficulties seem to revolve not around loss of ordering principles, but (1) reduction in syntactic complexity, (2) overuse of canonical word order as a "safe harbor," (3) blend errors in which a form appears in legal but semantically incorrect position, and (4) abandonment of the effort to produce a complete sentence under stressful conditions. We offer a redefinition of syntactic impairment as a problem in the access of phrase structure types, resulting in a preference for higher frequency forms. Parallels between lexical retrieval and phrase structure retrieval suggest that similar mechanisms may be at work in both cases.