Metagenome-Assembled Genomes from Monte Cristo Cave (Diamantina, Brazil) Reveal Prokaryotic Lineages As Functional Models for Life on Mars.

Microbial communities have been explored in various terrestrial subsurface ecosystems, showing metabolic potentials that could generate noteworthy morphological and molecular biosignatures. Recent advancements in bioinformatic tools have allowed for descriptions of novel and yet-to-be cultivated microbial lineages in different ecosystems due to the genome reconstruction approach from metagenomic data. Using shotgun metagenomic data, we obtained metagenome-assembled genomes related to cultivated and yet-to-be cultivated prokaryotic lineages from a silica and iron-rich cave (Monte Cristo) in Minas Gerais State, Brazil. The Monte Cristo Cave has been shown to possess a high diversity of genes involved with different biogeochemical cycles, including reductive and oxidative pathways related to carbon, sulfur, nitrogen, and iron. Three genomes were selected for pangenomic analysis, assigned as Truepera sp., Ca. Methylomirabilis sp., and Ca. Koribacter sp. based on their lifestyles (radiation resistance, anaerobic methane oxidation, and potential iron oxidation). These bacteria exhibit genes involved with multiple DNA repair strategies, starvation, and stress response. Because these groups have few reference genomes deposited in databases, our study adds important genomic information about these lineages. The combination of techniques applied in this study allowed us to unveil the potential relationships between microbial genomes and their ecological processes with the cave mineralogy and highlight the lineages involved with anaerobic methane oxidation, iron oxidation, and radiation resistance as functional models for the search for extant life-forms outside our planet in silica- and iron-rich environments and potentially on Mars.

The occurrence of microdiamonds in Mesoproterozoic Chapada Diamantina intrusive rocks--Bahia/Brazil.

The origin of diamonds from Serra do Espinhaço in Diamantina region (State of Minas Gerais) and in Chapada Diamantina, Lençóis region (State of Bahia) remains uncertain, even taking into account the ample research carried out during the last decades. The lack of typical satellite minerals in both districts makes a kimberlitic source for these diamonds uncertain. In mid 18th century the occurrence of a metamorphosed igneous rock composed of martite, sericite and tourmaline was described in Diamantina region and named hematitic phyllite, considered by some researchers as a possible diamond source. Similar rocks were found in Lençóis and examined petrographically and their heavy mineral concentration was investigated by means of scanning electron microscopy (SEM). Petrographic analyses indicated an igneous origin for these rocks and SEM analyses showed the discovery of microdiamonds. Geochronological studies using the Ar/Ar technique in muscovites yielded minimum ages of 1515+/-3 Ma, which may correlate with 1710+/-12 Ma from U-Pb method in igneous zircons from the hematitic phyllites. Both rock types also have the same mineral and chemical composition which leads to the conclusion that the intrusive rocks were protolith of the hematitic phyllites. This first discovery of microdiamonds in intrusive rocks opens the possibility of new investigation models for diamond mineralization in Brazilian Proterozoic terrains.