Understanding the relationship between weather variables and intake in beef steers.

The relationship between weather variables and dry matter intake (DMI) in beef steers was examined using daily intake data from 790 beef steers collected through a computer-controlled feeding system in nonsummer months. Daily data were condensed into weekly averages (N = 13,895 steer-weeks). The variables considered to predict DMI (2.50 to 23.60 kg/d) were body weight (197 to 796 kg), dietary net energy for maintenance (NEm; 0.79 to 2.97 Mcal/kg), ambient temperature (-23.73 °C to 21.40 °C), range of temperature (2.79 °C to 19.43 °C), dew point (-27.84 °C to 14.34 °C), wind speed (2.08 to 6.49 m/s), solar radiation (30.8 to 297.1 W/m2), and 2-wk lag (average of previous 2 wk's values) and monthly lag (average of previous 4 wk's values) of each weather variable. Toeplitz variance-covariance structure for repeated measures was used to determine the model to predict DMI, while accounting for the effects of body weight, dietary NEm, and other variables in the model. Two-week lag of ambient temperature interacted (P ≤ 0.005) with 2-wk lag of range of temperature, monthly lag of wind speed, 2-wk lag of solar radiation, and dew point to predict DMI. Interactions (P = 0.0001) between 2-wk lag of range of temperature vs. dew point and monthly lag of wind speed vs. 2-wk lag of solar radiation were also detected. This study reports important weather variables associated with differences in DMI of growing and finishing steers and will help improve the accuracy of DMI prediction equations for beef cattle. Improvements in the accuracy of predicting DMI should give producers better tools to plan and execute efficient feeding management programs. The R2 of the overall model was 0.8891.

Dry matter intake (DMI) models for beef cattle in the Northern Great Plains may not be a good fit because of extreme weather conditions experienced in this region. The objective of this study is to include additional weather variables (temperature, dewpoint, wind speed, range of temperature, and solar radiation as well as 2-wk lag and monthly lag for each weather variable) that may influence DMI models to improve accuracy. Intake data (13,895 observations) collected from 790 beef steers using an automatic feeding system from 2011 to 2018 was utilized. It is well-established that body weight and dietary energy density influences DMI in cattle, therefore, both were included in the base model. Weather variables that contributed the most to the model were 2-wk lag of range of temperature, ambient temperature, and solar radiation. There were also two-way interactions between most of the weather variables. This study shows that weather variables interact and current DMI models should account for these interactions. Ultimately, this will improve DMI models in the Northern Great Plains.

Late gestation supplementation of corn dried distiller's grains plus solubles to beef cows fed a low-quality forage: III. effects on mammary gland blood flow, colostrum and milk production, and calf body weights.

Objectives were to investigate the effects of supplementation with corn dried distiller's grains plus solubles (DDGS) to late gestating beef cows on arterial blood flow to the mammary glands during late gestation and early lactation; colostrum and milk production; dystocia and immunity; and calf BW. Cows were fed a control (CON; n = 15; 5.1% CP; 36.2% ADF) diet consisting of 90% corn stover and 10% corn silage on a dry basis offered ad libitum or CON diet with supplementation of DDGS (0.30% of BW; SUP n = 12). Mammary gland blood flow was assessed on day 245 of gestation. At parturition, maternal and calving parameters were assessed; colostrum and jugular blood was sampled; and dams were weighed. Mammary gland blood flow and milk production was measured on day 44 of lactation. Calves were weighed fortnightly for 8 wk and at weaning. Colostrum production tended to be greater in SUP dams than in CON dams (837 vs. 614 ± 95 g, P = 0.10). Calves of SUP dams were heavier at birth and 24 h (0 h, 43.2 vs. 39.8 ± 1.0 kg, P = 0.02; 24 h, 44.0 vs. 40.4 ± 1.1 kg, P = 0.02). At birth and 24 h, blood pCO2 was greater in calves born to SUP dams (6.82 vs. 6.00 ± 0.41 kPa, P = 0.04). Serum IgG did not differ (P = 0.21) at 24 h. Ipsilateral mammary gland blood flow of SUP cows was greater than CON cows (2.76 vs. 1.76 ± 0.30 L/min; P = 0.03); however, when summed with contralateral, total blood flow was similar (P = 0.33). Hemodynamic measures on day 44 of lactation were similar (P ≥ 0.32). Milk production tended to be increased (13.5 vs. 10.2 ± 1.2 kg/d, P = 0.07) in SUP vs. CON cows. Despite similar BW through 56 d, calves from SUP cows were heavier (P = 0.04) at weaning (309.7 vs. 292.0 ± 6.0 kg). In conclusion, we accept our hypothesis that DDGS supplementation during gestation influenced mammary blood flow, milk production and calf weights. These findings implicate maternal nutrition's leverage on both nutrient and passive immunity delivery to the calf early in life as well as potential advantages on long-term performance.

Effect of metabolizable protein intake on growth performance, carcass characteristics, and feeding behavior in finishing steers.

One-hundred thirty-two finishing steers (300 ± 2.7 kg body weight [BW]) predominately of Angus, Simmental, and Shorthorn breeding were used to study the effect of metabolizable protein (MP) intake on growth performance, carcass characteristics, and feeding behavior. Steers were stratified by initial BW across five pens and randomly assigned to one of four dietary treatments to supply an average of 626, 906, 1,209, and 1,444 g MP/d (n = 33 per treatment). Feed intake and feeding behavior were measured using radio frequency identification tags and the Insentec feeding system. For feeding behavior, a visit was defined as each time the Insentec system detected a steer at the feed bunk. A meal was defined as eating periods by intervals no longer than 7 min. Steers were fed until they reached an average BW of 598 ± 3.1 kg. Average daily gain (ADG) responded quadratically (P < 0.01) with ADG increasing in steers fed 906 g MP/d and plateauing thereafter. Dry-matter intake (DMI; kg) responded quadratically (P = 0.009) with DMI increasing with MP intake up to 1,209 g/d MP and decreasing thereafter. Gain to feed ratio (G:F) increased linearly (P = 0.04) and tended (P = 0.10) to respond quadratically, as G:F increased up to 906 g MP/d and plateaued thereafter. A quadratic response (P = 0.04 and P = 0.02, respectively) was observed for marbling score and 12th rib subcutaneous fat thickness with steers fed 1,209 g MP/d having the greatest marbling score and back fat thickness. A quadratic effect for visits and meals per day was observed (P < 0.01) with steers fed the 1,209 g MP/d treatment having the least visits and meals per day. In addition, time eating per visit responded quadratically (P = 0.05) with time increasing from 626 to 906 g MP/d. There was a linear increase (P ≤ 0.02) in time eating per meal and per day with increasing MP intake. A quadratic effect (P < 0.03) was observed for DMI per visit, meal, and minute with steers fed 1,209 g MP/d having the greatest DMI. In summary, steers fed 626 g MP/d had increased visits and meals per day. However, DMI per visit, meal, and minute were greater in steers fed 1,209 g MP/d. A day × treatment interaction (P < 0.001) was observed for plasma urea N as concentrations increased to a greater extent over time in the higher MP treatments than in the lower MP treatments. These data indicate that MP supply (from deficient to excess) influences growth performance, carcass characteristics, and feeding behavior of finishing steers.

Influence of feeding direct-fed microbial supplementation on growth performance and feeding behavior in naturally fed and conventionally fed finishing cattle with different dietary adaptation periods.

To determine the effects of finishing system (conventional vs. natural), dietary adaptation length (14 vs. 28 d), and direct-fed microbial (DFM) supplementation (no DFM vs. DFM) on growth performance and feeding behavior, 120 yearling steers (390 ± 2.8 kg) were used in a completely randomized design with a 2 × 2 × 2 factorial arrangement of treatments. Feed intake was monitored using the Insentec feeding system. Blood samples were collected every 28 d. After 140 d on feed, steers were slaughtered and carcass characteristics collected. Conventionally fed steers had greater (P ≤ 0.001) final BW, carcass weight, and dressing %. Dry matter intake was not influenced (P ≥ 0.31) by treatment. Length of dietary adaptation period did not influence (P ≥ 016) final BW, ADG, and G:F. There was a feeding system × DFM interaction (P ≤ 0.02) for ADG and G:F with conventionally fed steers fed DFM having the greatest (P ≤ 0.05) and naturally fed steers supplemented with DFM having the least (P ≤ 0.05) ADG and G:F. Number of visits to the feed bunk and number of meals per day did not differ (P > 0.05) among treatments. Time eating per visit and per meal was greater (P = 0.05) in steers supplemented with DFM than in steers not supplemented with DFM. On day 56 and 140, plasma glucose concentration was greater (P ≤ 0.03) in steers adapted in 14 d than in steers adapted in 28 d. On day 84, plasma glucose concentration was greater (P = 0.02) in naturally fed compared to conventionally fed steers. On day 112, there was a dietary adaptation period × DFM interaction (P = 0.004) for plasma glucose concentration with glucose concentration greatest (P ≤ 0.05) in steers adapted in 14 d supplemented with DFM and in steers adapted in 28 d not supplemented with DFM, least for steers adapted in 28 d supplemented with DFM, with steers adapted in 14 d not supplemented with DFM intermediate (P ≤ 0.05). On day 112 and 140, plasma urea N concentration was greater (P ≤ 0.05) in steers adapted in 28 d than in steers adapted in 14 d. These data indicate that conventionally fed steers generally had improved growth performance compared to naturally fed steers. Length of dietary adaptation and DFM supplementation had minimal effects on growth performance but did interact with feeding system to influence feeding behavior and blood metabolite concentrations.

Effect of post-insemination progesterone supplementation on pregnancy rate in dairy cows.

Progesterone plays an important role in maintenance of pregnancy. It is hypothesized that insufficient progesterone early in pregnancy may result in embryonic loss, and that supplemental progesterone would decrease pregnancy loss in dairy cows. In Experiment 1, 84 cows and 16 heifers from a single dairy operation were selected randomly. Within each age category, controlled internal drug release (CIDR) devices were inserted into the vagina of every other female on Day 4 post-insemination and removed on Day 18 post-insemination. Transrectal ultrasonography was performed to determine pregnancy at 4 time periods [days 30 to 37 (week 5), days 44 to 51 (week 7), days 58 to 65 (week 9), and days 86 to 93 (week 13)]. Progesterone supplementation had no effect on pregnancy rate. In Experiment 2, there were no differences in progesterone concentrations between cows that did and did not receive a CIDR. Further, cows receiving CIDR devices did not have an increase in circulating progesterone concentrations 30 min or 1 h after CIDR insertion. It appears that progesterone supplementation does not increase circulating levels of progesterone in the early pregnant lactating dairy cow. Alterative methods to influence progesterone concentrations and/or early embryonic loss need to be investigated.

Fate of chlorate present in cattle wastes and its impact on Salmonella Typhimurium and Escherichia coli O157:H7.

Chlorate salts are being developed as a feed additive to reduce the numbers of pathogens in feedlot cattle. A series of studies was conducted to determine whether chlorate, at concentrations expected to be excreted in urine of dosed cattle, would also reduce the populations of pathogens in cattle wastes (a mixture of urine and feces) and to determine the fate of chlorate in cattle wastes. Chlorate salts present in a urine-manure-soil mixture at 0, 17, 33, and 67 ppm had no significant effect on the rates of Escherichia coli O157:H7 or Salmonella Typhimurium inactivation from batch cultures. Chlorate was rapidly degraded when incubated at 20 and 30 degrees C with half-lives of 0.1 to 4 days. Chlorate degradation in batch cultures was slowest at 5 degrees C with half-lives of 2.9 to 30 days. The half-life of 100 ppm chlorate in an artificial lagoon system charged with slurry from a feedlot lagoon was 88 h. From an environmental standpoint, chlorate use in feedlot cattle would likely have minimal impacts because any chlorate that escaped degradation on the feedlot floor would be degraded in lagoon systems. Collectively, these results suggest that chlorate administered to cattle and excreted in wastes would have no significant secondary effects on pathogens present in mixed wastes on pen floors. Lack of chlorate efficacy was likely due to low chlorate concentrations in mixed wastes relative to chlorate levels shown to be active in live animals, and the rapid degradation of chlorate to chloride at temperatures of 20 degrees C and above.

Comparative effect of direct-fed microbials on fecal shedding of Escherichia coli O157:H7 and Salmonella in naturally infected feedlot cattle.

The effect of direct-fed microbials (DFM) on fecal shedding of Escherichia coli O157:H7 and Salmonella in naturally infected feedlot cattle was evaluated in a clinical trial involving 138 feedlot steers. Following standard laboratory methods, fecal samples collected from steers were evaluated for change in the detectable levels of E. coli O157:H7 and Salmonella shed in feces after DFM treatment. Sampling of steers was carried out every 3 weeks for 84 days. A significant reduction (32%) in fecal shedding of E. coli O157:H7 (P < 0.001), but not Salmonella (P = 0.24), was observed among the treatment steers compared with the control group during finishing. The probability of recovery of E. coli O157:H7 from the feces of treated and control steers was 34.0 and 66.0%, respectively. Steers placed on DFM supplement were almost three times less likely to shed E. coli O157:H7 (odds ratio, 0.36; 95% confidence interval, 0.25 to 0.53; P < 0.001) in their feces as opposed to their control counterparts. The probability of recovery of Salmonella from the feces of the control (14.0%) and the treated (11.3%) steers was similar. However, the DFM significantly reduced probability of new infections with Salmonella among DFM-treated cattle compared with controls (nontreated ones). It appears that DFM as applied in our study are capable of significantly reducing fecal shedding of E. coli O157:H7 in naturally infected cattle but not Salmonella. The factors responsible for the observed difference in the effects of DFM on E. coli O157:H7 and Salmonella warrants further investigation.

Reversible hepatotoxicity, pancreatitis, coagulation disorder and simultaneous bone marrow suppression with valproate in a 2-year-old girl.

Valproic acid (VPA) is considered to be a drug of first choice for the therapy of generalized and focal epilepsies, including special epileptic syndromes like the WEST-syndrome. The drug is usually well tolerated; rare serious complications may occur in some patients, including haemorrhagic pancreatitis, coagulapathies, bone marrow suppression, VPA-induced hepatotoxicity and encephalopathy. We report a case of combined appearance of several severe VPA-associated side effects in a two- and a half-year-old girl with lissencephaly.

Fecal shedding of Escherichia coli O157:H7 in North Dakota feedlot cattle in the fall and spring.

Cattle are an important reservoir of Escherichia coli O157:H7, which can lead to contamination of food and water, and subsequent human disease. E. coli O157:H7 shedding in cattle has been reported as seasonal, with more animals shedding during summer and early fall than during winter. North Dakota has relatively cold weather, especially in winter and early spring, compared with many other regions of the United States. The objective was to assess fecal shedding of E. coli O157:H7 in North Dakota feedlot cattle over the fall, winter, and early spring. One hundred forty-four steers were assigned randomly to 24 pens on arrival at the feedlot. Samples of rectal feces were obtained from each steer four times (October and November 2003, and March and April 2004) during finishing. On arrival (October 2003), 2 (1.4%) of 144 cattle were shedding E. coli O157:H7. The shedding increased significantly to 10 (6.9%) of 144 after 28 days (November 2003), to 76 (53%) of 143 at the third sampling (March 2004), and dropped significantly to 30 (21%) of 143 at the fourth (last) sampling (March 2004) before slaughter. Unfortunately, we were unable to sample the cattle during winter because of the extreme weather conditions. Sampling time significantly (P < 0.0001) influenced variability in E. coli O157:H7 shedding, whereas herd (P = 0.08) did not. The prevalence of E. coli O157:H7 shedding in North Dakota steers in fall and early spring was comparable to what has been reported in other parts of the United States with relatively warmer weather. Further research into E. coli O157:H7 shedding patterns during extreme weather such as North Dakota winters is warranted in order to fully assess the seasonal effect on the risk level of this organism.