RESUMO
In a series of pregnant women with iron deficiency anaemia treated by a total dose infusion of iron dextran, the non-haem iron content of the placenta at term was studied histochemically and by chemical analysis. Within a few days of the infusion the Prussian blue reaction on the placenta was very strong, but was negative by ten days after the infusion. Chemical analysis showed that both the water-insoluble fraction (haemosiderin) and the water-soluble fraction (ferritin) of the non-haem iron were increased soon after the infusion, but three weeks after the infusion they were almost the same as in untreated controls. Pinocytosis of iron dextran by the trophoblast and increased transport of transferrin-bound iron to the placenta are considered as possible causes for this large uptake of iron by the placenta.
Assuntos
Anemia Hipocrômica/tratamento farmacológico , Complexo Ferro-Dextran/uso terapêutico , Ferro/análise , Placenta/análise , Complicações na Gravidez/tratamento farmacológico , Feminino , Ferritinas/análise , Morte Fetal/metabolismo , Hemossiderina/análise , Humanos , Infusões Parenterais , Complexo Ferro-Dextran/administração & dosagem , GravidezRESUMO
A sensitive micro method for the manual estimation of true glucose in 10 mul plasma by an enzymatic technique is described. Protein precipitation is avoided. The method is simple to perform, accurate and precise. Agreement with a glucose oxidase autoanalyzer method is excellent, even with haemolysed and icteric specimens.
