RESUMO
A simple method for obtaining imaging documents of surgical specimens is reported. the method uses a scanner and a personal computer with printer. It provides rapid, low-cost high-quality images on paper or transparencies. The document can be used to illustrate a pathology report or discussion between clinicians and pathologists. The computers facilities already present in most Pathology laboratories is sufficient. The scanner with software cost slightly more than a camera.
Assuntos
Aumento da Imagem , Processamento de Imagem Assistida por Computador/métodos , Patologia Clínica/métodos , Humanos , Procedimentos Cirúrgicos OperatóriosRESUMO
368 formalin-fixed and paraffin-embedded carcinomas of the breast were studied by immunohistochemistry using antibodies to estrogen and progesterone receptors. The results, which are comparable to the results published in the literature, are discussed with particular emphasis on quality assurance parameters.
Assuntos
Neoplasias da Mama/química , Carcinoma Ductal de Mama/química , Imuno-Histoquímica/métodos , Receptores de Estrogênio/análise , Receptores de Progesterona/análise , Feminino , Humanos , Inclusão em ParafinaRESUMO
Three cases of small intestinal stromal tumors with skeinoid fibers are presented and are confronted to the 15 cases found in the literature. These tumors are characterized by the presence of intercellular eosinophilic globules that stain positively on periodic acid-Schiff stain. Ultrastructurally, these structures are composed of tangles of curved banded (45 nm) fibers simulating an appearance of skeins. By immunohistochemistry, tumor cells express vimentin only. These tumors seem to be derived from the enteric plexus.
Assuntos
Neoplasias Intestinais/ultraestrutura , Intestino Delgado/ultraestrutura , Idoso , Feminino , Humanos , Técnicas Imunoenzimáticas , Neoplasias Intestinais/química , Intestino Delgado/química , Masculino , Microscopia Eletrônica , Pessoa de Meia-IdadeRESUMO
The clinical and pathological features of three unusual soft tissue tumors are presented. They occurred in the groin of elderly patients in the subcutaneous tissue. In Case 1, the inguino-crural tumor coexisted with a second mass in the pelvis. The tumors had a tendency to recur locally and to invade the regional lymph nodes. Metastatic dissemination of the disease resulted in the death of the patient in Case 3. Histologically, the tumors appeared composed of small round cells with scanty cytoplasms, arranged in diffuse, poorly cohesive or solid sheets. In places, a trabecular pattern was noted. Mitotic figures were numerous. Some cells exhibited argyrophil granules. The ultrastructural study revealed compact whorls of intermediate filaments and neurosecretory granules. All three cases displayed a paranuclear dot-like positive reaction with antibodies to cytokeratins and neurofilaments. A diffuse cytoplasmic immunostaining for neuron-specific enolase was present in Cases 1 and 2. Protein S-100, vimentin and leucocyte common antigen could not be demonstrated. All these characteristics, except for the subcutaneous location, are shared with neuroendocrine (Merkel cell) tumors of the skin.
Assuntos
Carcinoma de Célula de Merkel/diagnóstico , Neoplasias Cutâneas/diagnóstico , Idoso , Idoso de 80 Anos ou mais , Antígenos de Diferenciação/metabolismo , Carcinoma de Célula de Merkel/metabolismo , Carcinoma de Célula de Merkel/patologia , Desmina/metabolismo , Diagnóstico Diferencial , Feminino , Antígenos de Histocompatibilidade/metabolismo , Humanos , Imuno-Histoquímica , Proteínas de Filamentos Intermediários/metabolismo , Queratinas/metabolismo , Antígenos Comuns de Leucócito , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Proteínas de Neurofilamentos , Fosfopiruvato Hidratase/metabolismo , Proteínas S100/metabolismo , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/patologia , Vimentina/metabolismoRESUMO
An antiserum to carcinoembryonic antigen (CEA) and a monoclonal antibody to cytokeratin 19 (CK 19) were studied for their suitability as diagnostic reagents for the differential diagnosis of primary and secondary malignant epithelial tumours of the liver, on paraffin sections. With the antiserum to CEA, positive bile canalicular structures were found in 60 per cent of the hepatocellular carcinomas. All the cholangiocarcinomas and 66.6 per cent of the metastatic carcinomas were positive for CEA, without displaying a canalicular staining pattern. All the hepatocellular carcinomas were negative for CK 19. All the cholangiocellular carcinomas and the metastatic carcinomas were positive for CK 19. This staining profile may prove helpful in difficult diagnostic cases.
Assuntos
Adenoma de Ducto Biliar/diagnóstico , Antígeno Carcinoembrionário/análise , Carcinoma Hepatocelular/diagnóstico , Queratinas/análise , Neoplasias Hepáticas/diagnóstico , Adenocarcinoma/diagnóstico , Adenocarcinoma/secundário , Diagnóstico Diferencial , Humanos , Neoplasias Hepáticas/secundárioRESUMO
A case of plexiform tumorlet of the uterus is reported and studied histologically and by immunohistochemistry. The presence of desmin in the tumour cells demonstrates their smooth muscle origin. These results are compared with other reports in the literature with special emphasis on histogenesis.
Assuntos
Neoplasias Uterinas/patologia , Adulto , Desmina/análise , Fator VIII/análise , Feminino , Humanos , Técnicas Imunoenzimáticas , Proteínas S100/análise , Neoplasias Uterinas/cirurgiaRESUMO
The authors report a case of intravascular hemangio-epithelioma vegetans of Masson. Usually a tumor of the head and neck. This case was characterized by the presence of two independent tumors on the same hand. On the cases of the 86 cases reported in the world literature, the authors suggest a primary endothelial origin for this benign tumor.