Progress in the research and demonstration of Everglades periphyton-based stormwater treatment areas.

The South Florida Water Management District (District) is conducting research focused on potential advanced treatment technologies to support reduction of phosphorus (P) loads in surface water entering the remaining Everglades. Periphyton-based stormwater treatment areas (PSTA) are one of the advanced treatment technologies being researched by the District. This detailed research and demonstration project is being conducted in two phases. Basic research in field-based mesocosm experiments was conducted during the first phase within the District's Everglades Nutrient Removal Project (ENR). Studies were conducted in 24 portable PSTA mesocosms and three of the south ENR test cells. Phase 1 studies addressed the effects of system substrate (shellrock, organic peat, or sand), water depth, hydraulic loading rate, vegetation presence, depth:width ratio, and inhibition of algal growth on total phosphorus removal performance of the PSTA mesocosms. A second phase of research is currently under way, during which PSTA feasibility will be evaluated further in four field-scale constructed mesocosms totaling about 2 ha, and follow up studies within the ENR test cells and portable mesocosms will be conducted to further investigate the effects of other inorganic substrates, shallow water depth, and velocity on treatment performance. Phase 1 monitoring has determined that periphyton-dominated communities can be established in constructed wetlands within 5 months. The algal component of these periphyton plant communities is characteristic of natural Everglades periphyton. High macrophyte densities resulted from use of peat soils in PSTA mesocosms, while shellrock and sand soils promoted more desirable sparse macrophyte stands. P removal rates under the conditions of this research were relatively high considering the low influent total P concentrations tested (average 23 microg/L). PSTA mesocosms on shellrock soils were able to attain long-term average outflow total P concentrations as low as 11 microg/L. The maximum one-parameter TP first-order removal rate constant (k1) measured was 27 m/y. Minimum attainable outflow total P concentrations and mass removals appear to be the result of a balance between internal P loading from antecedent soils, uptake and burial processes in new sediments, and rainfall inputs. A different soil type (limerock) will be tested for effectiveness during Phase 2. Selected existing treatments will also be continued to look for trends over a second growing season.

Evaluation of membrane adsorption-epifluorescence microscopy for the enumeration of bacteria in coastal surface films.

We have evaluated a method for enumerating surface slick bacteria by combining a membrane adsorption procedure with epifluorescence microscopy. Various chemicals were investigated for their ability to enhance bacterial elution from the membrane filters. The results of the elution-epifluorescence method were compared to plate counts and to direct epifluorescence counts of the sampling membrane filters. In all tests, the elution-epifluorescence technique yielded significantly higher bacterial concentrations.