First Complete Genomic Sequence of a Rabies Virus from the Republic of Tajikistan Obtained Directly from a Flinders Technology Associates Card.

A brain homogenate derived from a rabid dog in the district of Tojikobod, Republic of Tajikistan, was applied to a Flinders Technology Associates (FTA) card. A full-genome sequence of rabies virus (RABV) was generated from the FTA card directly without extraction, demonstrating the utility of these cards for readily obtaining genetic data.

[Use of fluorescent probes for identifying certain animal viruses and antibodies to them]. / Ispol'zovanie fliuorestsentnykh zondov dlia identifikatsii nekotorykh virusov zhivotnykh i antitel k nim.

Virus adsorption on cell receptors changed the state of cellular membranes, as was shown on a model of 25 viruses belonging to 13 families. This is indicated by an increase in the fluorescence of the DSM probe. Homologous antibodies block the virus adsorption on the cell membrane, this permitting identification of viruses and detection of antibodies to them.

[A rapid solid-phase immunoenzyme method in the diagnosis of viral infections]. / Uskorennyi tverdofaznyi metod v diagnostike virusnykh infektsii.

An accelerated solid-phase enzyme-immunoassay has been developed which permits identification of antigens and antibodies to them within 30-40 min, even directly at the site of specimen collection. The method was tested on the models of foot-and-mouth disease virus, vesicular disease of swine, vesicular exanthema of swine, Aujeszky's disease, leukemia, and coronavirus infection of cattle.

[The optimization of immunoenzyme analysis with conjugates of virus-specific antibodies and alkaline phosphatase]. / Optimizatsiia immunofermentnogo analiza s kon''iugatami virus-spetsificheskikh antitel i shchelochnoi fosfatazy.

The data obtained in the study of the dependence of sensitivity of enzyme immunoassay (EIA) on chromo- and fluorogenic substrates used in this assay are presented. The sandwich variant of EIA, carried out with the use of antibodies labeled with alkaline phosphatase, has been shown to be 4-170 times, sometimes 500 times, more sensitive (in terms of concentrations at which aphthous fever virus antigens can be detected) than the complement fixation test and 1.8-64 times more sensitive than the passive hemagglutination test.

[An immunoenzyme method of isolation of foot-and-mouth disease virus by using beta-lactamase conjugate with virus-specific antibodies]. / Immunofermentnyi metod opredeleniia virusa iashchura s ispo'lzovaniem ko''niugata beta-laktamazy s virisspetsifi- cheskimi antitelami.

It was shown that in was feasible to use conjugates of virus-specific antibodies and beta-lactamase from Bacillus licheniformis 749/c to identify aphthosa virus antigens. The antigen titers determined by enzyme immunoassay (EIA) using a beta-lactamase conjugate were 5-64 times higher than the analogous indices of the complement fixation test. Unlike EIA, that by using the antibody conjugates with peroxidase or alkaline phosphatase there were observed no "background" responses.