Normative data on horizontal power, acceleration, and change-of-direction ability in amateur youth female soccer players.

BACKGROUND: Physical performance measures such as horizontal power, acceleration, and change-of-direction ability are related to performance in soccer, and may aid in talent selection, coaching decisions and the prescription of training programs. The purpose of this study was to describe the normative values of three performance tests in amateur female youth soccer players. METHODS: A cross-sectional descriptive study design was employed with 151 amateur female youth soccer players (age 14.20±1.13 years). Physical performance measures included the standing broad jump, 10-meter sprint, and Pro-Agility Test. Time since peak height velocity (PHV) was calculated to represent physical maturity status. Descriptive statistics were sought, and Pearson's correlation coefficients were used to identify correlations between variables. One-Way Analysis of Variance with a Tukey post-hoc test was used to calculate the relationship between categorical groups of chronological ages and years since PHV. Significance was set at P<0.05. RESULTS: Normative data for each physical performance measure are presented based on chronological age and physical maturity status for amateur female youth soccer players. Chronological age was significantly associated with improved performance in standing broad jump (R=0.558; 95% CI: 0.436, 0.658; P<0.01), 10-meter sprint time (R=-0.375; 95% CI: -0.495, -0.217; P<0.01), and Pro-Agility Test time (R=-0.424; 95% CI: -0.546, -0.284; P<0.01). Physical maturity status was also significantly associated with improved performance in standing broad jump (R=0.650; 95% CI: 0.426, 0.711; P<0.01), 10-meter sprint time (R=-0.430; 95% CI: -0.507, -0.232; P<0.01), and Pro-Agility Test time (R=-0.453; 95% CI: -0.554, -0.293; P<0.01). CONCLUSIONS: The normative data from this study demonstrates differences in physical performances across chronological ages and physical maturity status. Physical maturity status had a slightly stronger association for all performance measures than chronological age. The variance and range tended to increase across performance measures with increasing age.

Corrigendum: Kinetic studies and CFD-based reaction modeling for insights into the scalability of ADC conjugation reactions.

[This corrects the article DOI: 10.3389/fbioe.2023.1123842.].

Kinetic studies and CFD-based reaction modeling for insights into the scalability of ADC conjugation reactions.

The manufacturing of antibody-drug conjugates (ADCs) involves the addition of a cytotoxic small-molecule linker-drug (= payload) to a solution of functionalized antibodies. For the development of robust conjugation processes, initially small-scale reaction tubes are used which requires a lot of manual handling. Scale-up to larger reaction vessels is often knowledge-driven and scale-comparability is solely assessed based on final product quality which does not account for the dynamics of the reaction. In addition, information about the influence of process parameters, such as stirrer speed, temperature, or payload addition rates, is limited due to high material costs. Given these limitations, there is a need for a modeling-based approach to investigate conjugation scale-up. In this work, both experimental kinetic studies and computational fluid dynamics (CFD) conjugation simulations were performed to understand the influence of scale and mixing parameters. In the experimental part, conjugation kinetics in small-scale reaction tubes with different mixing types were investigated for two ADC systems and compared to larger bench-scale reactions. It was demonstrated that more robust kinetics can be achieved through internal stirrer mixing instead of external mixing devices, such as orbital shakers. In the simulation part, 3D-reactor models were created by coupling CFD-models for three large-scale reaction vessels with a kinetic model for a site-specific conjugation reaction. This enabled to study the kinetics in different vessels, as well as the effect of process parameter variations in silico. Overall, it was found that for this conjugation type sufficient mixing can be achieved at all scales and the studied parameters cause only deviations during the payload addition period. An additional time-scale analysis demonstrated to aid the assessment of mixing effects during ADC process scale-up when mixing times and kinetic rates are known. In summary, this work highlights the benefit of kinetic models for enhanced conjugation process understanding without the need for large-scale experiments.

Women and Men Use Different Strategies to Stabilize the Head in Response to Impulsive Loads: Implications for Concussion Injury Risk.

BACKGROUND: Cervical musculoskeletal and neuromuscular attributes, which may vary between men and women, influence an individual's capacity to stabilize the head. OBJECTIVES: To examine sex differences in cervical musculoskeletal and neuromuscular attributes and their impact on head stability. The secondary objective was to examine the effects of anticipation and preload on head kinematics. METHODS: Thirty-four (20 men, 14 women) recreationally active adult athletes completed a perturbation protocol with anticipation and preloading conditions in this descriptive cross-sectional study. We assessed the neuromuscular response of the sternocleidomastoid to perturbation and head kinematics. We measured neck girth, sternocleidomastoid physiological cross-sectional area, and isometric strength. RESULTS: Women had smaller neck girth, smaller sternocleidomastoid physiological cross-sectional area, and lower isometric strength than men. Women had greater baseline electromyography (EMG) amplitude and greater peak EMG response than men. There were no sex differences in sternocleidomastoid onset latency or head kinematics. Women had a greater increase in baseline EMG amplitude after preloading and anticipated conditions. Preloading attenuated sex differences in muscle onset latency. Across the sexes, there was a significant main effect of anticipation on head kinematics. CONCLUSION: Men and women used different strategies to stabilize the head, and responded differently to the preloading and anticipation conditions. J Orthop Sports Phys Ther 2019;49(11):779-786. Epub 15 May 2019. doi:10.2519/jospt.2019.8760.

Characterization of cervical neuromuscular response to head-neck perturbation in active young adults.

BACKGROUND: The majority of studies examining the role of cervical muscles on head-neck kinematics focused on musculoskeletal attributes (e.g. strength). Cervical neuromuscular response to perturbation may represent a divergent construct that has not been examined under various perturbation conditions. This study examined the association between cervical musculoskeletal attributes and cervical neuromuscular response of the sternocleidomastoid (SCM) to perturbation. Furthermore, this study examined the effect of anticipation and preload on the SCM neuromuscular response. METHODS: Nineteen participants completed measurement of SCM muscle size, cervical flexion maximal voluntary isometric contraction, and the neuromuscular response of the SCM to cervical perturbation. Cervical perturbation was delivered by dropping a 1.59 kg mass from a loading apparatus. The impulsive load was delivered under four conditions: (1) Anticipated perturbation with no preload (A-NP), (2) Unanticipated perturbation with no preload (U-NP), (3) Anticipated perturbation with preload (A-P), and (4) Unanticipated perturbation with preload (U-P). RESULTS: None of the cervical musculoskeletal attributes were correlated with the SCM cervical neuromuscular response. This study demonstrated significant effect of preloading and anticipation on baseline EMG amplitude and EMG onset latency for the SCM. Furthermore, there was a significant effect of preloading on average EMG response amplitude for the SCM. DISCUSSION: The findings of this study indicate that cervical neuromuscular response of the SCM is different from musculoskeletal attributes and is influenced by perturbation conditions. These findings provide conceptual support to examine the neuromuscular response of the SCM in mitigating head-neck kinematics.

Performance and reliability of the Y-Balance Test™ in high school athletes.

BACKGROUND: Lower extremity injuries account for 32.9% of the overall injuries in high school athletes. Previous research has suggested that asymmetry greater than 4 cm using the Y-Balance Test™ Lower Quarter (YBT-LQ) in the anterior direction is predictive of non-contact injuries in adults and collegiate athletes. The prevalence of asymmetries or abnormal YBT-LQ performance is not well documented for adolescents. The primary purposes of this study are: 1) to characterize the prevalence of YBT-LQ asymmetries and performance in a cross-sectional sample of adolescents; 2) to examine possible differences in performance on the YBT-LQ between male and female adolescents; and 3) to describe the test-retest reliability of the YBT-LQ in a subsample of adolescents. METHODS: Observational cross-sectional study. High-school athletes completed the YBT-LQ as main outcome measure. RESULTS: A total of 51 male and 59 female high-school athletes participated in this study. Asymmetries greater than 4cm in the posteromedial (PM) reach direction were most prevalent for male (54.9%) and female (50.8%) participants. Females presented with slightly higher composite scores. Good reliability (ICC=0.89) was found for the anterior (ANT) direction, and moderate reliability with 0.76 for posterolateral (PL) and 0.63 for PM directions. The MDC95 for the ANT direction was 6% and 12% for both the PL and PM directions. CONCLUSIONS: The YBT-LQ performance can be beneficial in assessing recovery in an injured extremity compared to the other limb. However, due to the large MDC95, noted in the PM and PL directions, the differences between sequential testing cannot be attributed to true change in balance unless they exceed the MDC95. In this study, 79% of the athletes presented with at least one asymmetry in YBT-LQ reach distances. Moderate reliability in the PL and PM directions warrants reexamination of the definition of asymmetry in these directions.

Performance of high school male athletes on the Functional Movement Screen™.

OBJECTIVES: (1) Describe the performance of the Functional Movement Screen™ (FMS™) by reporting the proportion of adolescents with a score of ≤14 and the frequency of asymmetries in a cross-sectional sample; (2) explore associations between FMS™ to age and body mass, and explore the construct validity of the FMS™ against common postural stability measures; (3) examine the inter-rater and test-retest reliability of the FMS™ in adolescents. DESIGN: Cross-sectional. SETTING: Field-setting. PARTICIPANTS: 94 male high-school athletes. MAIN OUTCOME MEASURE: The FMS™, Y-Balance Test (YBT) and Balance Error Scoring System (BESS). RESULTS: The median FMS™ composite score was 16 (9-21), 33% of participants scored below the suggested injury risk cutoff composite score of ≤14, and 62.8% had at least one asymmetry. No relationship was observed between the FMS™ to common static/dynamic balance tests. The inter-rater reliability of the FMS™ composite score suggested good reliability (ICC = 0.88, CI 95%:0.77, 0.94) and test-retest reliability for FMS™ composite scores was good with ICC = 0.83 (CI 95%:0.56, 0.95). CONCLUSIONS: FMS™ results should be interpreted cautiously with attention to the asymmetries identified during the screen, regardless of composite score. The lack of relationship between the FMS™ and other balance measures supports the notion that multiple screening tests should be used in order to provide a comprehensive picture of the adolescent athlete.

A Therapeutic Uricase with Reduced Immunogenicity Risk and Improved Development Properties.

Humans and higher primates are unique in that they lack uricase, the enzyme capable of oxidizing uric acid. As a consequence of this enzyme deficiency, humans have high serum uric acid levels. In some people, uric acid levels rise above the solubility limit resulting in crystallization in joints, acute inflammation in response to those crystals causes severe pain; a condition known as gout. Treatment for severe gout includes injection of non-human uricase to reduce serum uric acid levels. Krystexxa® is a hyper-PEGylated pig-baboon chimeric uricase indicated for chronic refractory gout that induces an immunogenic response in 91% of treated patients, including infusion reactions (26%) and anaphylaxis (6.5%). These properties limit its use and effectiveness. An innovative approach has been used to develop a therapeutic uricase with improved properties such as: soluble expression, neutral pH solubility, high E. coli expression level, thermal stability, and excellent activity. More than 200 diverse uricase sequences were aligned to guide protein engineering and reduce putative sequence liabilities. A single uricase lead candidate was identified, which showed low potential for immunogenicity in >200 human donor samples selected to represent diverse HLA haplotypes. Cysteines were engineered into the lead sequence for site specific PEGylation and studies demonstrated >95% PEGylation efficiency. PEGylated uricase retains enzymatic activity in vitro at neutral pH, in human serum and in vivo (rats and canines) and has an extended half-life. In canines, an 85% reduction in serum uric acid levels was observed with a single subcutaneous injection. This PEGylated, non-immunogenic uricase has the potential to provide meaningful benefits to patients with gout.

PERFORMANCE OF HIGH SCHOOL FOOTBALL PLAYERS ON CLINICAL MEASURES OF DEEP CERVICAL FLEXOR ENDURANCE AND CERVICAL ACTIVE RANGE OF MOTION: IS HISTORY OF CONCUSSION A FACTOR?

BACKGROUND: More than one million adolescent athletes participated in organized high school sanctioned football during the 2014-15 season. These athletes are at risk for sustaining concussion. Although cervical spine active range of motion (AROM) and deep neck flexor endurance may serve a preventative role in concussion, and widespread clinical use of measurements of these variables, reference values are not available for this population. Cost effective, clinically relevant methods for measuring neck endurance are also well established for adolescent athletes. PURPOSE: The purpose of this study was to report reference values for deep cervical flexor endurance and cervical AROM in adolescent football players and examine whether differences in these measures exist in high school football players with and without a history of concussion. METHODS: Concussion history, cervical AROM, and deep neck flexor endurance were measured in 122 high school football players. Reference values were calculated for AROM and endurance measures; association were examined between various descriptive variables and concussion. RESULTS: No statistically significant differences were found between athletes with a history of concussion and those without. A modest inverse correlation was seen between body mass and AROM in the sagittal and transverse planes. CONCLUSION: The results of this study indicate that the participants with larger body mass had less cervical AROM in some directions. While cervical AROM and endurance measurements may not be adequate to identify adolescents with a history of previous concussions among high school football players. However, if a concussion is sustained, these measures can offer a baseline to examine whether cervical AROM is affected as compared to healthy adolescents. LEVEL OF EVIDENCE: 2c.