Economic viability of using OvSynch and fixed timed artificial insemination protocol in breeding improvement of pastoral herds in the rangelands.

Though using Assisted Reproductive Technologies (ARTs) can improve oestrus detection, conception and pregnancy success, thus benefit breeding program implementation, empirical evidence of their economic viability is lacking to inform investment decisions in pastoral herds. This study assessed economic viability of using OvSynch and fixed Timed Artificial Insemination (TAI) protocol in Sahiwal upgrading breeding program under two hypothetical cases of best and worst in activity-based money allocations when pastoral herds deploy either optimal or low input husbandry practices. From herd owners' assessment of milk production, best-case scenarios attain on average 10 L/cow/day with optimal husbandry and 5 L/cow/day with low input husbandry. The worst-case scenarios attain 5 L/cow/day with optimal husbandry and 1 L/cow/day with low input husbandry. Benefit- Cost Analysis (BCA) estimated Net Present Value (NPV), Benefit-Cost ratio (BCR) and Internal Rate of Return (IRR) to establish economic viability of using OvSynch and TAI Protocol in pastoral breeding programs. Both best-case scenarios retuned positive NPVs (82,028 and 6,912), BCR values (1.68 and 1.08) and IRR (27.46% and 8.08%) while worst-case scenarios returned negative NPVs (-135,855 and -141,025), BCR values of below 1 (0.87 and 0.66) and IRR values below the minimum rate of returns. These economic parameters were sensitive to price changes in inputs and outputs, under both optimal and low input husbandry practices. Results indicate that using OvSynch and TAI Protocol is a profitable and economically viable investment under optimal husbandry practices but not under low input husbandry practices. By implications, use of OvSynch and TAI Protocol in Sahiwal upgrading breeding programs need be accompanied with improved husbandry practices and de-risking pastoral herd owners from price changes in input and output markets.

Influence of endometritis on milk yield of zero-grazed dairy cows on smallholder farms in Rwanda.

Endometritis being a post-partum uterine infection in dairy cows is likely with substantial production loss through reduction in milk yield (MY), discarded milk during treatment and withdrawal period, and increased cost of veterinary treatment. This study quantified the influence of endometritis on MY of zero-grazed dairy cows managed on smallholder farms in Rwanda. The study enrolled a total of 461 cows within their 21 to 60 days in milk to examine for clinical endometritis (CLE) and subclinical endometritis (SCLE). A cow was considered having endometritis if it was positive for at least one test (CLE or SCLE), otherwise was negative. The MY data were collected prospectively from endometritis positive and negative cows for 30-day post-endometritis diagnosis. Compared to cows negative for endometritis, the positive endometritis cows were 2.4 times more (29.7 vs. 70.3%) with daily MY 15.3% lower (7.5 ± 0.2 vs. 8.9 ± 0.3 litres; p<0.05), representing a reduction of 1.4 ± 0.2 litres of milk/cow/day. Of the CLE positive cows, 33.4% (104/311) were treated using different veterinary drugs, which resulted in 23.5% more discarded milk compared (p<0.05) to untreated positive cows. Discarded milk was higher (p<0.05) among cows treated with oxytetracycline (65.9 ± 4.4 litres) compared to cows treated with procaine penicillin G and dihydrostreptomycin (35.5 ± 2.7 litres). The percentage of total milk loss was much higher (45.6%) among CLE positive cows that received treatment compared to the untreated cows (16.3%). These results demonstrate a strong association between MY loss and endometritis. A timely diagnosis and treatment of the disease is recommended using conventional veterinary drugs that have zero withholding time for milk to reduce the MY loss and associated economic loss, estimated at 154 US$ in a lactation.

Porcine Cysticercosis Control in Western Kenya: The Interlink of Management Practices in Pig Farms and Meat Inspection Practice at Slaughter Slabs.

This study assessed the management practices for controlling porcine cysticercosis (PC) on pig farms and in pork at the slaughter slabs in two counties (Busia and Kakamega) of Western Kenya. A total of 162 pig-rearing households at the farm level, 26 butcher owners, and 26 slaughter slab workers at the slaughter slab level were interviewed using a structured questionnaire. Data were analyzed using the "Statistical Analysis System" (SAS) programme. Results indicated that the frequent management practices used at the farm level (p < 0.05) were rearing pigs under free range (69.1%), latrine ownership by households (87.7%), and use of pit latrines (72.8%) in households. At the slaughter level (p < 0.05), results of the butcher owners (76.9%) and slaughter slab workers (62.5%) revealed that meat inspection was not practiced adequately in the two areas of study. The results imply that slaughtered pigs for human consumption were not adequately inspected, and thus, the study recommends for implementation of effective pig management practices at the farm level and pork meat inspection at slaughter slabs to prevent PC infections and assure food safety along the pork value chain.

Effects of endometritis on reproductive performance of zero-grazed dairy cows on smallholder farms in Rwanda.

Endometritis is a prevalent post-partum uterine infection in dairy cows resulting in suboptimal reproductive performance. The hypothesis was that endometritis status is associated with suboptimal reproductive performance of postpartum dairy cows managed under zero-grazing feeding practice on smallholder farms. In this study, there was evaluation of effects of endometritis diagnosed at 38.5 ±â€¯14.7 days postpartum (dpp) on subsequent reproductive performance. Reproductive performance of 436 cows from 345 farms was recorded for 210 dpp. Values for reproductive performance indicators were less (P < 0.05) in cows determined to be positive compared to negative for endometritis. Cows that tested positive, as compared to negative, for endometritis had longer periods after parturition until initiation of oestrous cycles (median, interquartile range; 85.0, 57.5-127.0 and 62.6, 49.0-90.0 days, respectively), longer durations before being detected pregnant (95.5, 61.8-145.5 and 63.0, 50.0-83.0 days, respectively), lesser pregnancy rates as a result of the first breeding postpartum (16.5% and 32.7%, respectively), more natural-mating or artificial inseminations per pregnancy (1.3 ± 0.1 and 1.1 ± 0.0, respectively) and more occurrences of anoestrus postpartum (48.4% and 11.7%, respectively). These results provide evidence of a strong association between endometritis and suboptimal fertility performance in zero-grazed cows on smallholder farms in Rwanda. Considering there were 70.2% of cows in the present study were diagnosed with endometritis, this is indicative of a widespread herd health issue, warranting that field veterinary practitioners prioritise endometritis in their herd health service delivery to smallholder dairy farmers for effective disease management and herd sustainability.

Estimating prevalence of endometritis in smallholder zero-grazed dairy cows in Rwanda.

Endometritis is a postpartum uterine disease of cows that interrupts reproductive cycles resulting in suboptimal fertility, reduced performance and profitability of the dairy herd. The objective of the study was to estimate the perceived and observed prevalence of endometritis among zero-grazed dairy cows in smallholder farms in Rwanda. A snowball sampling method was applied in cross-sectional survey to obtain data from 370 farms on 466 cows within their 21-60 days postpartum (dpp). The survey, conducted from September 2018 to March 2019, simultaneously examined cows using the Metricheck Device (MED) to determine the presence and type of vaginal mucus (VMC) based on a score scale of 0 to 3. Cows scoring VMC ≥ 1 were recorded as clinical endometritis (CLE)-positive. Cytotape (CYT) was used to determine the percentages of polymorphonuclear cells (PMN) in endometrial cytology sample. Cows with ≥ 5% PMN were recorded subclinical endometritis (SCLE)-positive, whereas cows with VMC-0 and < 5% PMN were considered healthy cows. At cow-level, endometritis prevalence was 70.2% with 67.2% CLE and 31.8% SCLE while at the herd-level, prevalence was 71.1% with 68.1% CLE and 34.4% SCLE. The differences between the diagnostic performance of the MED and CYT were significant (p < 0.001). Perceived prevalence by farmers was much lower (3.2%) and without agreement with the observed prevalence (kappa = - 0.02, p > 0.05). The highly observed prevalence and farmer underestimation of endometritis prevalence indicate knowledge gaps about endometritis. The extension service therefore needs to increase awareness and education among smallholder farmers about detection and management of endometritis.

Perception of farmers about endometritis prevention and control measures for zero-grazed dairy cows on smallholder farms in Rwanda.

BACKGROUND: Endometritis is a prevalent uterine disease in postpartum cows. The disease reduces fertility performance and milk yield, and subsequently, productivity and profitability of dairy farms. The reduction in performance is associated with considerable economic losses on dairy farms. Smallholder farmers are likely to incur considerable economic losses from the disease where they lack knowledge of effective prevention and control measures for the disease. This study used farmer's perspectives to determine the effectiveness of different management interventions (MIs) for endometritis prevention and control on smallholder farms in Rwanda practicing dairy zero-grazing. The best-worst scaling (BWS) choice method was applied that relied on past 1 year recall data obtained from 154 farmers. These farmers were identified through snowball sampling in a cross-sectional study. RESULTS: Of the 20 MIs evaluated, 12 scored highly for effectiveness. The top four most effective are: avoiding sharing equipment with neighbouring farms (45.5%), consulting animal health service provider about disease treatment (31.8%), keeping cows in a clean and dry shed (26.7%), and selecting sires based on calving ease (26.6%). The MIs considered least effective were: maintaining clean transition cow housing (35.1%), removal of fetal membrane immediately after passing (33.1%), disinfecting the equipment used in calving assistance before and after use (32.5%), and selecting sires with low percent stillbirths (29.2%). CONCLUSION: This study has demonstrated the application of BWS object case method in understanding the MIs that farmers consider are most effective in the prevention and control of endometritis disease in the dairy herds. The MIs are on-farm biosecurity and hygiene, seeking veterinary services for disease treatment and selecting sires for ease of calving. These MIs should be considered for prioritization in extension services and research to continuously improve and enhance their practical application on smallholder dairy farms.

PREVALENCE OF PORCINE CYSTICERCOSIS AMONG SCAVENGING PIGS IN WESTERN KENYA.

BACKGROUND: Porcine Cysticercosis (PC) infection is globally classified as a neglected and re-emerging tropical disease. The disease is endemic in Western Kenya yet smallholder farmers continue to practice scavenging pig production, thereby posing public health risk. This study determined the prevalence of PC infection at the farms and slaughter slabs in a cross-sectional survey in two Counties (Busia and Kakamega) of Western Kenya. MATERIALS AND METHODS: Two hundred and eighty-seven (287) heparinized blood samples were collected at the farm from 162 households in 9 villages and 113 pigs from 5 slaughter slabs. The prevalence of PC was detected through meat inspection at slaughter slabs, and the prevalence of Taenia solium antigen determined by using the ApDia Ag-ELISA test at the farms and slaughter slabs. RESULTS: At meat inspection, the PC prevalence was 1.8%, while prevalence of Taenia Species cysts detected with Ag-ELISA test was 3.8% at the farms, and 5.3 % at the slaughter slabs. The Ag-ELISA test had sensitivity of 100% (95% CI: 19.79- 100.00) and specificity of 96.4% (95% CI: 90.49- 98.84). CONCLUSION: The PC prevalence levels observed among scavenging pigs in Western Kenya should be a cause of public health risk concern. This observation warrant enforcing mandatory pig confinement, and use of latrines at the farms and meat inspection at local slaughter slabs. Further studies are recommended to identify different Taenia species in cysticercoids pigs in the region, which this study could not differentiate.

PORCINE CYSTICERCOSIS RISKS: AWARENESS, ATTITUDES AND PERCEPTIONS ON SAFETY PRACTICES AMONG FARMERS, BUTCHER-OWNERS AND CONSUMERS IN WESTERN KENYA.

BACKGROUND: The demand for pork is increasing in Africa with the increasing need for animal protein in the household diets. But pork safety and quality remains a pervasive concern that needs intervention to assure consumers of protection from Porcine Cysticercosis (PC) contamination. This study assessed among farmers, butcher-owners and consumers in Western Kenya about their awareness, attitudes and perceptions about safety practices regarding risk of PC. MATERIALS AND METHODS: Data were obtained using structured questionnaires in cross-sectional survey interviews with 162 farmers, 26 butcher-owners and 92 consumers from Busia and Kakamega Counties. The data were in binary response, so were analyzed with Chi - square test. RESULTS: Only two in ten farmers had knowledge of Taenia solium parasite (24.1%), risk factors in PC transmission (21.6%) and could associate pig management system with PC (17.3%). A larger proportion (p<0.01) of the butcher owners perceived pork from slaughter slabs (76.9%) and home slaughters (73.1%) as presenting high risks but considered pork from the butcheries (69.1%) and eateries (61.5%) as presenting no risks. Among the consumers, majority strongly agreed (p<0.05) that pork in the market (85.9%), from slaughter slabs (92.4%) and butchery (81.5%) was safe but a larger proportion strongly disagreed that pork from the eateries exposed them to cysticercosis (64.1%). CONCLUSION: The awareness about risks of PC was low among farmers. Butcher-owners and consumers perceived pork safety differently along the value chain. Strengthening public education about PC risks and pork safety among all actors in the pork value chain in Western Kenya is recommended.

Improving the nutritive value of wheat straw with urea and yeast culture for dry season feeding of dairy cows.

The study evaluated the effects of feeding urea treated/supplemented wheat straw-based diets with addition of yeast culture (YC) as a dry season feed for dairy cows. Wheat straw diets with 3.6% urea and 5.8% molasses were formulated to upgrade nonprotein nitrogen levels and fibre degradation in the rumen. Yeast culture was included at 0 and 10 g/cow/day in mixer with commercial dairy meal to improve on fibre degradation and milk yield. Two experiments were conducted. Firstly, an in sacco dry matter degradability (DMD) trial with three steers in a completely randomized design (CRD) with a 3 × 2 factorial arrangement to determine the effects on intake and rumen degradation parameters. Secondly, feeding trial with 18 lactating cows in a 3 × 2 factorial arrangement at two levels of yeast culture (0 and 10 g/cow/day) and three types of urea interventions: No intervention (WS); addition of urea to straw at the time of feeding (USWS); and 7 days incubation of straw with urea (UTWS). Yeast cultures addition had no effect on rumen pH and NH3-N, but urea intervention showed an effect on rumen pH with USWS being lowest (p < 0.05). Both urea interventions and yeast culture addition had no effect (p > 0.05) on dry matter intake, milk yield, and milk composition but they increased (p < 0.05) propionate yields.