RESUMO
Two aquatic moss species, Calliergon megalophyllum and Drepanocladus sordidus (Amblystegiaceae, Bryophyta), which had been considered extinct in the Czech Republic, were found in the Trebon Basin, South Bohemia, in 2016-2017. They co-occurred in extensive reed- and sedge-dominated fen pools with humic water on the shore of an old fishpond and the former species was also discovered in a small humic pool in an old shallow sand-pit. The new Czech sites of these rare boreal species represent one of the southernmost known outposts within their entire European range. Previously, the two species were only known from single records in the Czech Republic from the late 19th and early 20th centuries. To confirm our morphological observations, we used phylogenetic analyses of DNA sequence variation in four chloroplast loci (atpB-rbcL, trnL-trnF, rpl16, trnG) and one nuclear region, the internal transcribed spacers of ribosomal DNA (ITS). We found (1) monophyly of all Calliergon megalophyllum specimens tested; (2) based on chloroplast DNA sequences, monophyly among all Drepanocladus sordidus specimens and representatives of Pseudocalliergon turgescens and P. lycopodioides moss species; (3) based on nuclear ITS sequences, monophyly of all original D. sordidus specimens. These results corroborate morphological studies and thus confirm the existence of natural sites for the studied moss species in the Trebon Basin, South Bohemia, Czech Republic.
RESUMO
The mitogenome of Niphotrichum ericoides (GenBank accession number KP 233863) has a total length of 106,727 bp and consists of 40 protein-coding genes, three ribosomal RNAs (rRNAs), and 24 transfer RNAs. The gene order is identical to that known in other moss mitogenomes.
Assuntos
Briófitas/classificação , Briófitas/genética , Genoma Mitocondrial , Composição de Bases , Biologia Computacional/métodos , Genes Mitocondriais , Tamanho do Genoma , Fases de Leitura Aberta , Análise de Sequência de DNA , Sequenciamento Completo do GenomaRESUMO
The mitogenome of the Codriophorus laevigatus (GenBank accesion number KM506905) has a total length of 106,809 bp and consist of 40 protein-coding genes, 3 ribosomal RNA (rRNA) and 24 transfer RNA. The gene order is identical to other known moss mitogenomes.