Effects of sodium hypochlorite gel and sodium hypochlorite solution on dentin bond strength.

The aim of this study was to determine the effect of 10% NaOCl gel and 10% NaOCl solution on dentin bond strengths of four adhesive systems. One hundred eighty bovine incisors were ground to achieve a flat polished surface, then divided into 12 groups: Gluma One Bond [G1-control; G2-NaOCl solution; G3-NaOCl gel]; Prime & Bond 2.1 [G4-control; G5-NaOCl solution; G6-NaOCl gel]; Single Bond [G7-control; G8-NaOCl solution; G9-NaOCl gel]; Prime & Bond NT [G10-control; G11-NaOCl solution; G12-NaOCl gel]. Dentin was etched, rinsed, and blot dried. For the experimental groups, after acid etching, 10% NaOCl solution or 10% NaOCl gel was applied for 60 s, rinsed, and blot dried. Composite resin was inserted and light cured. Shear bond strengths were tested with a crosshead speed of 0.5 mm/min. The mean values MPa (SD) were analyzed with two-way ANOVA and Tukey's tests (alpha < 0.01). Ten percent NaOCl solution significantly increased Gluma One Bond strength. No effect was observed for the other adhesives. The 10% NaOCl gel did not affect bond strengths. Ten percent NaOCl gel was less effective on collagen removal as compared to 10% NaOCl solution. The influence of collagen removal on bond strength is dependent on adhesive system, where both the solvent and the monomer can influence the results.

Long-term bond strength of restorations subjected to thermo-mechanical stresses over time.

PURPOSE: To evaluate the long-term effect of thermal and mechanical cycling on dentin bond strength to cervical margins of Class II restorations. METHODS: Sixty Class II slot cavities were prepared in bovine incisors. The cavities were restored with Single Bond and Z-250 composite according to manufacturer's instructions. The teeth were then divided into two groups (n = 30): specimens that would receive thermo-mechanical load cycling (2,000 cycles 5-55 degrees C/100,000 cycles of 50 N loading) (TM) and the control (C). Fifteen specimens from each group were tested at baseline and the remaining 30 specimens were stored in distilled and deionized water at 37 degrees C and tested after 1 year. For microtensile evaluation, the restorations were sectioned perpendicular to the cervical bonded interface into 0.7 +/- 0.2 mm thick slabs. The slabs were further trimmed at the interface to 1.4 < or = 0.2 mm with a fine diamond bur to produce a cross-sectional surface area of ca. 1 mm2. Specimens were then subjected to microtensile bond testing. The bond strength data were analyzed using two-way ANOVA and Fisher's PLSD test (P < 0.05). Fracture mode analysis was performed using SEM. RESULTS: At baseline, bond strength of the TM group was significantly lower when compared to the C group (P = 0.012). However, after 1 year storage, a significant decrease in bond strength was observed for the C group compared to baseline. No significant differences were noted between the C and TM groups at 1 year. No interaction was observed between groups (C and TM) and storage time (P = 0.098). For the fracture mode evaluation, at baseline, mixed failure was predominant for the C group, and increased after TM. Decreased mixed failure was observed after 1-year storage, with a concomitant increase in interphase failure.

Influence of load cycling and tubule orientation on ultimate tensile strength of dentin.

PURPOSE: The purpose of this study was to evaluate the effect of mechanical load cycling and tubule orientation on the ultimate tensile strength of crown and root dentin. MATERIALS AND METHODS: Twenty bovine teeth were cleaned and their incisal surfaces reduced, resulting in a flat surface 4 mm above the cementoenamel junction. The teeth were divided into 4 groups: G1 = control (no mechanical load cycling); G2 = 1,000,000 load cycles at 50 N; G3 = 1,000,000 load cycles at 100 N; G4 = 1,000,000 load cycles at 200 N. Sections of 0.5+/-0.1 mm thick were cut mesiodistally and the slabs further trimmed to 0.5+/-0.1 mm in an hourglass shape, according to the tubule orientation (parallel and/or perpendicular to the long axis of the tooth) in the root and crown, with a fine diamond bur to produce a cross-sectional surface area of 0.25 mm2. All specimens were then subjected to ultimate tensile strength testing at a crosshead speed of 1 mm/min. Means and standard deviations were expressed in MPa. The bond strength data were analyzed with three-way ANOVA and Fisher's PLSD test (p < 0.05). RESULTS: Ultimate tensile strength values did not differ statistically significantly between the loaded group and the control. However, statistical differences existed between root and coronal dentin (p < 0.001) and between parallel and perpendicular orientation of tubules (p < 0.001), where root and sections parallel to the tubules presented higher values than coronal and perpendicular, respectively. These differences were not related to mechanical loading. CONCLUSION: There was no effect of in vitro load cycling on ultimate tensile strength of dentin. Differences presented between ultimate tensile strength of crown vs root dentin and parallel vs perpendicular to the tubule orientation can be influenced by differences in the amount of organic and inorganic content as well as composition of the dentin matrix. It is important to consider these aspects when predicting the quality and durability of restorations according to the types of dentin and tubule orientation to be restored.

Effect of thermal and mechanical load cycling on nanoleakage of Class II restorations.

PURPOSE: To evaluate the effect of thermal and mechanical cycling on the degree and pattern of nanoleakage on cervical margins of Class II restorations. MATERIALS AND METHODS: Forty box-type Class II cavities were prepared on bovine incisors. The cavities were restored with Single Bond and Z-250 composite resin (3M-ESPE) according to manufacturer's instructions. The teeth were randomly assigned to 4 groups: G1, control; G2, thermal cycling (2000 cycles, 5 to 55 degrees C); G3, mechanical load cycling (100,000 cycles, 50 N); G4, thermal and mechanical load cycling group (2000 cycles 5 to 5 degrees C/100,000 cycles, 50 N). The specimens were then sealed leaving a 1 mm window around the cervical margin interface. Samples were immersed in a 50% w/v ammoniacal silver nitrate solution for 24 h, and exposed to a photodeveloping solution for 8 h. Specimens were sectioned longitudinally, embedded in epoxy resin, polished and mounted on stubs, gold sputter coated, and examined under SEM using backscattered electron mode. Silver particle penetration length was measured directly on the SEM monitor and calculated as the percentage of the total length of cut dentin surface that was penetrated by silver nitrate. The data were analyzed with ANOVA and Fisher's PLSD test (p < 0.05). RESULTS: The degree of nanoleakage significantly increased when thermal and mechanical cycling was performed on the same specimens, as compared to the other groups (p < 0.05). No differences were observed between the control, thermal cycling, and mechanical cycling groups. No difference in nanoleakage pattern was observed between the groups. CONCLUSION: Thermal and mechanical cycling combined adversely affected nanoleakage values. Simulation of the oral condition might be crucial to better evaluate and understand the performance of adhesive materials.