RESUMO
BACKGROUND: Epidemiological studies suggest that dietary intake of vitamins A, C and E may be associated with the occurrence of asthma. A systematic review and meta-analysis was conducted in accordance with MOOSE guidelines to determine whether vitamins A, C and E, measured as dietary intakes or serum levels, are associated with asthma. METHODS: MEDLINE, EMBASE, CINAHL, CAB abstracts and AMED (up to November 2007), conference proceedings and bibliographies of papers were searched to identify studies of asthma, wheeze or airway responsiveness in relation to intakes and serum concentrations of vitamins A, C and E. Pooled odds ratios (OR) or mean differences (MD) with 95% confidence intervals (CI) were estimated using random effects models. RESULTS: A total of 40 studies were included. Dietary vitamin A intake was significantly lower in people with asthma than in those without asthma (MD -82 microg/day, 95% CI -288 to -75; 3 studies) and in people with severe asthma than in those with mild asthma (MD -344 microg/day; 2 studies). Lower quantile dietary intakes (OR 1.12, 95% CI 1.04 to 1.21; 9 studies) and serum levels of vitamin C were also associated with an increased odds of asthma. Vitamin E intake was generally unrelated to asthma status but was significantly lower in severe asthma than in mild asthma (MD -1.20 microg/day, 95% CI -2.3 to -0.1; 2 studies). CONCLUSIONS: Relatively low dietary intakes of vitamins A and C are associated with statistically significant increased odds of asthma and wheeze. Vitamin E intake does not appear to be related to asthma status.
Assuntos
Antioxidantes/administração & dosagem , Asma/etiologia , Dieta/efeitos adversos , Vitaminas/administração & dosagem , Ácido Ascórbico/administração & dosagem , Ácido Ascórbico/sangue , Asma/sangue , Humanos , Vitamina A/administração & dosagem , Vitamina A/sangue , Vitamina E/administração & dosagem , Vitamina E/sangueRESUMO
Explant cultures of embryonic chick sternum have been widely studied, but the kinetics of biosynthesis of proteoglycans by this tissue in culture has not been characterized. Caudal cartilaginous portions of 16-day-old embryonic chick sterna were cultured for 8 days. Histological examination showed that the fresh cartilage contained morphologically homogenous chondrocytes, which were embedded in a uniform extracellular matrix. After culture for 8 days, the histological appearance of the explant remained unchanged but the tissue increased in size with time as indicated by a progressive increase in DNA content and in the content of glycosaminoglycan and collagen. Rates of degradation and release from the tissue of proteoglycans labeled in ovo with 35S were first order during culture, as were the unlabeled proteoglycans. Proteoglycan synthesis was high during the first 2 days of culture, and this then gradually decreased from this high level during the following 2 days. Synthesis was then maintained at a constant level for the remainder of the culture period. After culture for 2 and 7 days, the proteoglycans synthesized by the explants were identical to the preexisting proteoglycans in hydrodynamic size, glycosaminoglycan chain size, and ability to form aggregates. These findings suggest that the embryonic chick sterna maintained a stable cartilage phenotype during the extended culture periods. The initial rapid rate of matrix turnover was probably attributable to an adaptation of the tissue to ex ovo culture conditions and the subsequent maintenance of cellular activities at a lower level indicated the establishment of a steady-state rate of metabolism.
Assuntos
Cartilagem/embriologia , Proteoglicanas/química , Esterno/embriologia , Animais , Cartilagem/anatomia & histologia , Cartilagem/metabolismo , Células Cultivadas , Embrião de Galinha , Cromatografia , Colágeno/análise , DNA/análise , Glicosaminoglicanos/análise , Cinética , Proteoglicanas/análise , Esterno/anatomia & histologia , Esterno/metabolismo , Fatores de TempoRESUMO
The influence of pulsed electromagnetic fields (PEMF) on proteoglycan composition in cartilage extracellular matrix has been investigated. Day 16 embryonic chick sternal cartilage was explanted to culture and exposed for 3 h per day for 2 days to a repetitive single-pulse PEMF with frequency of 15 Hz and peak magnetic field of 1.25 G. PEMF treatment did not affect cell proliferation, as indicated by [3H]thymidine incorporation, but significantly stimulated the retention of glycosaminoglycans in the explants and reduced the release of glycosaminoglycans into the media. Determination of incorporation of [35S]sulfate and [3H]N-acetylglucosamine into proteoglycans in vitro and breakdown of in ovo labelled [35S]sulfated proteoglycans in vitro showed that PEMF treatment significantly suppressed the synthesis of proteoglycans and the degradation of both newly synthesized and pre-existing proteoglycans. Sepharose CL-2B chromatography demonstrated that PEMF did not affect either the size distribution of newly synthesized and pre-existing [35S]sulfated proteoglycans or their ability to aggregate with hyaluronate. Sepharose CL-6B chromatography followed by cellulose acetate electrophoresis revealed that the chain length and degree of sulfation of [35S]sulfated glycosaminoglycans were identical in control and PEMF-treated cultures. It is concluded that PEMF treatment preserved extracellular matrix integrity of cultured cartilage explants by down-regulating proteoglycan synthesis and degradation in a co-ordinated manner without affecting their gross structural nature.
Assuntos
Cartilagem/química , Campos Eletromagnéticos , Matriz Extracelular/efeitos da radiação , Proteoglicanas/análise , Animais , Cartilagem/embriologia , Embrião de Galinha , Colágeno/análise , DNA/biossíntese , Matriz Extracelular/química , Glicosaminoglicanos/análise , Técnicas de Cultura de Órgãos , Proteoglicanas/metabolismo , EsternoRESUMO
PURPOSE: During the early stages (embryonic day 3 [E3]) of avian corneal development, nerve fibers extend from the trigeminal ganglion to the corneal limbus. On E11, these nerve fibers enter the cornea and extend through the secondary stroma to begin innervation of the epithelium on E13. This process of innervation is concomitant with the cornea's dehydration and transition from opacity to transparency; thus, suggesting a link between innervation and the attainment of corneal function. This investigation attempts to ascertain whether the developing cornea can support its innervation in vitro and whether there is a possible developmental interrelationship between corneal innervation and dehydration, with the associated transition from opacity to transparency. METHODS: Isolated corneas from either E8 or E14 chicks were co-cultured with E8 dorsal root ganglia. After 4 days of culture, innervation was visualized by silver staining and immunohistochemistry. Changes in corneal composition and organization associated with this innervation in vitro were analyzed by measuring changes in specific hydration, thickness and compaction, and incorporation of [35S]sulfate into glycosaminoglycans during co-culture. RESULTS: The E8 and E14 corneas support extensive innervation in vitro. Developing nerve fibers extend through the secondary stroma to innervate the epithelium. In vitro innervation of E8, but not E14, corneas was associated with a decrease in corneal specific hydration, whereas control corneas (without dorsal root ganglia) failed to show any such changes. E8 corneas also showed a significant increase in compaction when innervated in vitro. Corneal innervation in vitro did not significantly change the overall incorporation of [35S]sulfate into glycosaminoglycans. Furthermore, incorporation of [35S]sulfate into corneal sulfated glycosaminoglycans (sGAG) is not influenced by either the number of nerve fibers innervating the cornea or nerve growth factor (NGF). In addition, the distribution of staining of the corneal glycosaminoglycans, chondroitin sulfate and keratan sulfate, and peanut agglutinin-binding epitopes, suggests that these molecules are not associated with inhibition of axonal development. CONCLUSIONS: The in vitro system described here is a useful model to understand the process of corneal development. Co-culture has shown that corneal innervation promotes the process of dehydration, which is dependent on the age of the cornea. However, other functionally related refinements necessary for transparency-notably proteoglycan synthesis-may not be linked to innervation or NGF production. The authors conclude that the development of transparency is dependent on corneal innervation, though not exclusively, and that other controlling factors also are required.
Assuntos
Córnea/embriologia , Córnea/inervação , Glicosaminoglicanos/biossíntese , Fibras Nervosas/fisiologia , Gânglio Trigeminal/embriologia , Animais , Embrião de Galinha , Técnicas de Cocultura , Córnea/citologia , Gânglios Espinais/citologia , Gânglios Espinais/fisiologia , Glicosaminoglicanos/análise , Imuno-Histoquímica , Cinética , Fibras Nervosas/ultraestrutura , Técnicas de Cultura de Órgãos , Sulfatos/metabolismo , Fatores de TempoRESUMO
Pulsed electromagnetic fields (PEMF) influence the extracellular matrix metabolism of a diverse range of skeletal tissues. This study focuses upon the effect of PEMF on the composition and molecular structure of cartilage proteoglycans. Sixteen-day-old embryonic chick sterna were explanted to culture and exposed to a PEMF for 3 h/day for 48 h. PEMF treatment did not affect the DNA content of explants but stimulated elevation of glycosaminoglycan content in the explant and conserved the tissue's histological integrity. The glycosaminoglycans in sterna exposed to PEMF were indistinguishable from those in controls in their composition of chondroitin sulfate resulting from chondroitinase ABC digestion. Specific examination with [35S]-sulfate labels showed that PEMF treatment significantly suppressed both the degradation of pre-existing glycosaminoglycans biosynthetically labeled in ovo and the synthesis of new [35S]-sulfated glycosaminoglycans. The average size and aggregating ability of pre-existing and newly synthesized [35S]-sulfated proteoglycans extracted with 4 M guanidinium chloride from PEMF-treated cartilage explants were identical to controls. The chain length and degree of sulfation of [35S]-sulfated glycosaminoglycans also were identical in control and PEMF-treated cultures. PEMF treatment also reduced the amount of both unlabeled glycosaminoglycans and labeled pre-existing and newly synthesized [35S]-sulfated glycosaminoglycans recovered from the nutrient media. [35S]-Sulfated proteoglycans released to the media of both control and PEMF-treated cultures were mostly degradation products although their glycosaminoglycan chain size was unchanged. These results demonstrate that exposure of embryonic chick cartilage explants to PEMF for 3 h/day maintains a balanced proteoglycan composition by down-regulating its turnover without affecting either molecular structure or function.
Assuntos
Cartilagem Articular/efeitos da radiação , Campos Eletromagnéticos , Matriz Extracelular/efeitos da radiação , Glicosaminoglicanos/biossíntese , Proteoglicanas/biossíntese , Animais , Cartilagem Articular/embriologia , Cartilagem Articular/metabolismo , Embrião de Galinha , Técnicas de Cultura , DNA/metabolismo , DNA/efeitos da radiação , Matriz Extracelular/metabolismo , Fluorometria , Glicosaminoglicanos/efeitos da radiação , Proteoglicanas/efeitos da radiação , Esterno/embriologia , Esterno/metabolismo , Esterno/efeitos da radiaçãoRESUMO
A simple technique has been devised for generating consistent microscalpel blades from tungsten wire for use in microdissections. Electrolysis of the tungsten wire held horizontally in contact with the extended, up-lifted meniscus of an aqueous solution of 1.0 N NaOH sculps the metal into a blade configuration with a very sharp cutting edge. These microscalpels are suitable for dissection of embryonic neural tissues.
Assuntos
Dissecação/instrumentação , Eletrólise , Ciência de Laboratório Médico , Tungstênio , Álcalis , SoluçõesRESUMO
Growth of the embryonic chick cornea was directly related to, and coordinated with, overall eye growth. During normal development, the size of the embryonic chick cornea increased in three linear phases of diametric growth. Corneal diameter increased at a rate of 216 microns per day between embryonic day 4 (E4) and E7, 511 microns per day between E7 and E10, and 144 microns per day from E10 until after hatching. After the sustained release of intraocular pressure by intubation on E4, corneal diametric growth was reduced to a single phase of 122 microns per day. After intubation on E4, the mesenchyme surrounding the developing cornea was substantially thicker and the neural crest-derived corneal endothelium was established earlier. The primary corneal stroma of the intubated eye swelled and was precociously populated by neural crest-derived corneal fibroblasts. Thus, the timing of arrival of neural crest cells in the anterior segment and their contribution to the cornea were determined by the growth rate of the eye. Although the diameter of the cornea was substantially reduced after intubation, it was more densely populated by fibroblasts, resulting in a cornea that was substantially thicker than the control by E14. Prospective corneal nerves normally extend into the cornea proper on E11, concomitant with a decrease in its diametric growth rate. After intubation on E4, the perilimbal nerve ring was virtually complete by E5 and numerous nerves had extended throughout the E8 cornea. By E16, the cornea from the intubated eye contained a very high density of nerve fibers, possibly reflecting its reduced size. These data suggest that the primary corneal stroma does not permit nerve fiber extension and demonstrate that the timing of nerve fiber extension into the secondary corneal stroma is specified by the rate of oppositional diametric growth of the cornea.
Assuntos
Córnea/embriologia , Pressão Intraocular , Animais , Embrião de Galinha , Córnea/inervação , Desenvolvimento Embrionário e Fetal , Intubação , Sistema Nervoso/embriologiaRESUMO
The pattern and relative rates of diametric growth of the avian eye and cornea are described throughout embryonic development. The effect of reduced intraocular pressure on eye and corneal diametric growth also was investigated. Between embryonic day 4 (E4) and 1 day posthatching, the eye undergoes two distinct phases of linear growth. The first phase (E4-10) is very rapid (1.193 mm/day). The second phase, after E10, is significantly slower (0.346 mm/day). By contrast, over the same developmental period, the cornea undergoes three distinct and sequential phases of linear growth. The second phase of corneal growth (E7-10) is the most rapid (0.429 mm/day) and separates two periods of slow growth (0.211 mm/day during E4-7 and 0.128 mm/day after E10). After the sustained release of intraocular pressure by intubation on E4, growth of both the eye and cornea is reduced significantly. Operated eyes grow at a rate of 0.356 mm/day (E4-10) and 0.155 mm/day (E10-16). Intubation reduces corneal growth to a single phase of 0.125 mm/day (E7-16). Thus, from E4-10 both the eye and cornea possess intrinsic growth potentials that are elevated significantly by intraocular pressure. After E10, the rate of growth of both the eye and the cornea is independent of intraocular pressure. Because both control and intubated eyes change their growth rate on E10, this transition also is independent of intraocular pressure. This contrasts with the cornea which, after intubation, shows no detectable variation in growth rate. Correlation of eye with corneal growth demonstrates an exponential relationship in the presence of intraocular pressure and an almost linear relationship after intubation.
Assuntos
Embrião de Galinha/fisiologia , Córnea/embriologia , Desenvolvimento Embrionário e Fetal , Olho/embriologia , Pressão Intraocular , Animais , IntubaçãoRESUMO
To examine the mechanism of intercellular adhesion in the establishment of limb skeletal elements we have investigated the process of limb bud cell aggregation in vitro. Limb bud cells are aggregation-competent immediately after their trypsin:collagenase dissociation in the absence of calcium. This aggregation is largely Ca2(+)-independent (CI) and is completely and reversibly inhibited by cycloheximide. In contrast, when limb bud cells are first allowed to recover from Ca2(+)-free trypsin:collagenase dissociation, aggregation of the surviving population is exclusively Ca2(+)-dependent (CD) and completely and reversibly inhibited by cycloheximide. The presence of exogenous calcium during initial cell dissociation retains a functional CD aggregation mechanism. However, incubation of such cells with EGTA releases the CD component and converts the cells to a predominantly CI aggregation. Rabbits were immunized with limb bud cells exhibiting the recovered CD aggregation mechanism and the resulting immune sera were screened for their effect on cell aggregation. Relative to pre-immune sera, intact immune IgG agglutinated dissociated limb bud cells whilst immune Fab fragments inhibited their aggregation. The aggregation-inhibiting antiserum recognizes five major limb bud cell surface components with apparent molecular weights of 72K, 50K, 23K, 14.5K and 8.5K (K = 10(3) Mr), respectively. Limb bud cell surface plasma membranes were isolated by sucrose gradient density centrifugation and detergent-solubilized proteins coupled to Sepharose 4B with cyanogen bromide. Equivalent cell surface plasma membrane proteins were 125I-iodinated and applied to the affinity column. Limb bud cell surface protein affinity chromatography in the presence of exogenous calcium yields a single protein with an apparent molecular weight of approximately 8.5 K. This protein molecule elutes at 0.6 M NaCl, indicating a high affinity, is recognized by the aggregation-inhibiting antiserum, and is itself capable of inhibiting CD limb bud cell aggregation. Fab fragments prepared from rabbit antisera specifically directed against the affinity-purified material also inhibit CD limb bud cell aggregation and this inhibition is neutralized by the 8.5 K protein. Our data thus demonstrate that CD limb bud cell aggregation is not mediated by fibronectin and/or collagen type I and indicate that this process is governed by a novel 8.5 K cell adhesion molecule.
Assuntos
Cartilagem/embriologia , Adesão Celular/fisiologia , Agregação Celular/fisiologia , Extremidades/embriologia , Animais , Cálcio/farmacologia , Cartilagem/citologia , Adesão Celular/efeitos dos fármacos , Moléculas de Adesão Celular/isolamento & purificação , Moléculas de Adesão Celular/fisiologia , Agregação Celular/efeitos dos fármacos , Embrião de Galinha , Técnicas de Cultura , Peso MolecularRESUMO
The digital flexor tendons of the neonate and adult horse have been compared with respect to variation in extracellular matrix composition along their length. Two pepsin-sensitive, acetic acid soluble proteins, molecular weight (Mr) 52 kD (np 52) and Mr 54 kD (np 54), were prominent throughout the length of neonatal tendons. In adult tendon, np 52 and np 54 were less abundant and restricted to the cannon (metacarpal) region. In contrast, a single pepsin- and collagenase-resistant protein of Mr 55 kD (fp 55) was exclusive to the fetlock (metacarpophalangeal joint) region regardless of age, although more distinct in the adult. Pepsin extracted fp 55 precipitated at 2.0 M sodium chloride: 0.5 M acetic acid and was further purified to homogeneity by bacterial collagenase digestion. Analysis of fp 55 amino acid composition revealed the presence of a large proportion of glycine residues (379 of 1001), suggesting a possible homology with the collagen family. These data demonstrate that the composition of equine digital flexor tendons varies with age, is heterogeneous along its length, and suggests that variation in tendon extracellular matrix composition is influenced by functional requirements.
Assuntos
Envelhecimento , Matriz Extracelular/análise , Cavalos/anatomia & histologia , Tendões/análise , Acetatos , Ácido Acético , Aminoácidos/análise , Animais , Animais Recém-Nascidos , Pepsina A/metabolismo , Proteínas/análise , SolubilidadeRESUMO
Within the avian cornea collagen type IV is preferentially and characteristically localized to the epithelial and endothelial basement membranes. In the present paper, we demonstrate that collagen type IV also is present within the corneal stroma coincident with the development and distribution of corneal nerves indicating that intra-stromal fibers are associated with Schwann cells or an equivalent cell type. We also demonstrate intra-stromal fibers of collagen type IV orthogonal to the epithelial basement membrane. These novel structures are most prominent on the tenth day of development and become progressively less distinct until they are no longer detectable on the eighteenth day of development. Substance P immunoreactivity is prominently expressed by nerves innervating the epithelium. The first substance P immunoreactive nerves are detected on the twelfth day of development, concomitant with the initiation of epithelial innervation and not the extension of nerves through the stroma. Such nerve fibers become more numerous with progressive development and demonstrate extensive association with both basal and superficial epithelial cells. Thus, the avian cornea is richly supplied with substance P primary afferents. The expression of substance P immunoreactivity correlates directly with the initiation of innervation of the corneal epithelium.
Assuntos
Colágeno/metabolismo , Córnea/inervação , Sistema Nervoso/metabolismo , Substância P/imunologia , Animais , Embrião de Galinha , Colágeno/classificação , Substância Própria/metabolismo , Imuno-Histoquímica , Sistema Nervoso/embriologia , Sistema Nervoso/imunologia , Distribuição TecidualRESUMO
The developing avian cornea is enclosed within a nerve ring from which numerous neurites extend into the cornea as radially ingrowing, regularly bifurcating fascicles. The authors now assess the degree of positional specificity of developing corneal nerves by photographic comparison and digital computer signal averaging. Such analyses demonstrate that nerves display extensive homology of position within the developing nerve ring, indicating that their growth cones originally followed specific pathways around the cornea. During their extension within the cornea, nerve fascicles bifurcate only within distinct, successive, and concentric zones suggesting that the position of an intra-corneal nerve branch point is designated by the intra-corneal milieu. Since the distance between the branch zones reported here increases with corresponding corneal growth, neurite elongation may occur both at the growth cone and along the axon at inter-branch sites of extending corneal nerves.
Assuntos
Córnea/inervação , Sistema Nervoso/embriologia , Animais , Embrião de Galinha , Córnea/embriologia , Fatores de TempoRESUMO
We have previously reported the isolation of a hydrophobic, type-II collagen-binding glycoprotein of molecular weight 31,000 (31,000-mol-wt protein) from chick chondrocyte membranes (Mollenhauer, J., and K. von der Mark, EMBO Eur. Mol. Biol. Organ. J., 2:45-50). The function of this protein in anchoring pericellular type II collagen to the chondrocyte surface was inferred from its ability to bind native type-II collagen either when detergent solubilized or when inserted into liposomes. In the present study we have used specific antibodies to localize this protein, which we now call anchorin CII, to the surface of chondrocytes in both cartilage sections, and in cell culture. In immunofluorescence studies of isolated chondrocytes we observed a dense, punctate distribution of anchorin CII on the cell surface when chondrocytes were enclosed by a pericellular type II collagen matrix. Removal of the pericellular matrix with trypsin also removed anchorin CII. The membrane protein character of anchorin CII was indicated by the demonstration of antibody-induced patching and capping on the chondrocyte surface at 22 degrees C and 37 degrees C, respectively. In monolayer culture, the amount of anchorin CII appeared reduced on flattened chondrocytes lacking a pericellular type II collagen matrix but was prominent upon intercellular cell processes. Fab' fragments prepared from either anchorin CII antiserum or an antiserum directed against the entire chondrocyte membrane inhibited the attachment of chondrocytes to a type II collagen substrate. In each case, the inhibition of attachment was neutralized by preincubation of Fab' fragments with purified anchorin CII.
Assuntos
Cartilagem/metabolismo , Colágeno/metabolismo , Receptores de Superfície Celular/metabolismo , Animais , Complexo Antígeno-Anticorpo , Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Galinhas , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Soros Imunes , Cinética , Receptores de ColágenoRESUMO
The cell body, neurite and growth cone of isolated retinal neurons have been compared on the basis of their ability to bind a number of fluorescently labelled lectins, each possessing a unique carbohydrate specificity. The susceptibility of the respective binding patterns following pretreatment of these fixed cells with either neuraminidase or trypsin was also investigated. Neuronal cell bodies displayed the most intense binding of each lectin, with localization of limulin binding (specific for sialic acid) predominantly to the neurite hillock, the point on the cell body from which the neurite projects. Limulin binding was almost totally abolished by pretreatment with either neuraminidase or trypsin. In contrast to the cell body, limulin binding to the neurite or growth cone was not detected. These regions of the cell apparently possessed sialic acid, however, since pretreatment with neuraminidase reduced wheat germ agglutinin binding (to N-acetylglucosamine) and markedly enhanced Dolichos biflorus agglutinin binding (to N-acetylgalactosamine) to both the neurite and growth cone. The initially low binding of Dolichos biflorus agglutinin to the neurite and growth cone was slightly enhanced by pretreatment with trypsin. Uniformly low levels of binding of either Ricinus communis agglutinin 60 (galactose, N-acetylgalactosamine) or R. communis agglutinin 120 (galactose) was observed over the entire neuron. R. communis agglutinin 120 binding was not enhanced by pretreatment with neuraminidase. Receptors for either concanavalin A (mannose, glucose) or Ulex europaeus agglutinin I (fucose) were abundant over the entire nerve cell with the former exhibiting more marked trypsin sensitivity. From these data, it is apparent that the repertoire of lectin binding sites of the neurite and growth cone of these differentiating nerve cells differs markedly from that of the cell body, which itself demonstrates some degree of regionalization.
Assuntos
Lectinas de Plantas , Receptores Mitogênicos/metabolismo , Retina/metabolismo , Animais , Células Cultivadas , Embrião de Galinha , Concanavalina A/metabolismo , Haptenos/farmacologia , Lectinas/metabolismo , Neuraminidase/farmacologia , Neurônios/metabolismo , Ricina/metabolismo , Tripsina/farmacologia , Aglutininas do Germe de TrigoRESUMO
The distribution of binding sites for four rhodamine-conjugated lectins has been examined in paraffin-embedded tissue sections of the developing and mature avian neural retina. The method of fixation employed here (95% ethanol:glacial acetic acid) is shown to conserve substantially more in vivo labeled 3H-glycoprotein or 3H-ganglioside than fixation with either 3.7% formaldehyde or 2% glutaraldehyde. Each lectin was shown to be optimally active by direct hemagglutination and inhibitable by the appropriate hapten in the cytochemical assay. Staining with wheat germ agglutinin (leads to N-acetylglucosamine) occurs predominantly to each of the anuclear retinal layers and progressively disappears from each of the nuclear layers. By adulthood, the inner nuclear layer is stained in a gradient which is highest adjacent to the inner nuclear layer while the nuclear layers are virtually unstained. Although similar to wheat germ agglutinin, staining with concanavalin A (leads to mannose) is less intense and persists in the adult nuclear layers. Dolichos biflorus agglutinin (leads to N-acetylgalactosamine) predominantly stains the embryonic ganglion cell layer. With development, this lectin demonstrates profound differences between the major cell types of the inner nuclear layer and the inner (negative) and outer (positive) plexiform layers. Differences between the principal synaptic layers are further accentuated by Ulex europaeus agglutinin I (leads to fucose): Binding sites are virtually absent from the 12th day embryonic retina, but are abundant throughout the retina by the 18th day and persist after hatching. However, in the adult retina staining persists in the outer plexiform layer but is lost from the inner plexiform and nerve fiber layers. In contrast to Dolichos biflorus agglutinin, Ulex europaeus agglutinin I stains the inner nuclear layer uniformly. Temporal differences in the characteristic lectin binding patterns exhibited by each of the major nuclear and anuclear layers of the developing avian neural retina are thus documented.