RESUMO
Current PCR assays for the differential diagnosis of malaria need to be further developed and certified in the Russian Federation. PCR may serve as a control diagnostic method. These investigations have demonstrated that an erythrocytic clot may be used as an adjunct to microscopy and the described procedure can be employed to isolate DNA for PRC in an inpatient setting.
Assuntos
DNA de Protozoário/isolamento & purificação , Malária/sangue , Malária/diagnóstico , Plasmodium falciparum/isolamento & purificação , China , DNA de Protozoário/sangue , DNA de Protozoário/genética , Humanos , Malária/genética , Malária/parasitologia , Plasmodium falciparum/genética , Reação em Cadeia da Polimerase , Federação RussaRESUMO
The technique for isolating blastocysts in the culture was assessed, by examining the fresh specimens from 196 patients with gastrointestinal tract (GIT) disorders and from 56 persons with an uncertain protozoological diagnosis. A set of techniques, such as triple examination of native smears, parallel inoculation of the Pavlova medium, and microscopy of obtained permanent specimens as may be required, contributes to the timely identification of protozoa, including blastocysts, in persons with GIT disorder. The efficiency of the technique for isolating blastocysts on Pavlova's medium and the native smear test averaged 34.8% and 49.4%, respectively. Among those known to be infected, the rate of parasite development in vitro was not greater than 52.9%. The culture showed amoeboids generally with the clinical manifestation of infection. The culture-based diagnostic method cannot be changed for routine microscopy techniques in detecting blastocysts, although it is essential to investigate the morphofunctional and phylogenetic properties of the parasite. The pleomorphism of cultured blastocysts with variability of growth properties was shown as a manifestation of phenotypic characteristics when isolating the parasite.
Assuntos
Infecções por Blastocystis/diagnóstico , Blastocystis/crescimento & desenvolvimento , Trato Gastrointestinal/parasitologia , Adulto , Blastocystis/classificação , Blastocystis/isolamento & purificação , Infecções por Blastocystis/parasitologia , Criança , Meios de Cultura , Fezes/parasitologia , Humanos , Microscopia , FilogeniaRESUMO
The details of imported case of Chesson strain of P. vivax malaria to Russian Federation (Moscow) from border areas between Myanmar and Thailand is given. It might further confirm the expansion of this strain from its original place in the Western Pacific towards other territories in the World, described in various publications.
