RESUMO
Alginate-chitosan-PLGA composite microspheres encapsulating outer membrane protein antigen of Aeromonas hydrophila as an antigen carrier was explored for the first time in a fish model. This composite microsphere showed distinct advantages over the conventional PLGA microparticles in aspects of the high encapsulation efficiency due to the protein-friendly microenvironment created by the hydrophilic alginate-chitosan cores of the composite microspheres, preventing initial burst release and the elimination of lyophilizing process. The antibody responses significantly increased and persist up to 9 weeks in composite microspheres unlike that PLGA microsphere, native OMP and FIA adjuvant. Moreover, several innate immune parameters as respiratory burst, lysozyme and complement activity were significantly increased in both composite and PLGA microspheres up to 9 weeks than other treated groups. It also gives protection from A. hydrophila infection and brought some hope, for its application in replacement with conventional PLGA microparticle for antigen delivery in fish.
Assuntos
Aeromonas hydrophila/imunologia , Alginatos/química , Quitosana/química , Infecções por Bactérias Gram-Negativas/veterinária , Ácido Láctico/química , Microesferas , Ácido Poliglicólico/química , Imunidade Adaptativa , Animais , Anticorpos Antibacterianos , Cyprinidae , Doenças dos Peixes/microbiologia , Doenças dos Peixes/prevenção & controle , Ácido Glucurônico/química , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/prevenção & controle , Ácidos Hexurônicos/química , Imunidade Inata , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Canais de Ânion Dependentes de VoltagemRESUMO
Surface modified poly-É-caprolactone microspheres as an antigen carrier was explored in a fish model. Outer membrane vesicles of Edwardsiella tarda adsorbed on to surface modified poly-É-caprolactone microspheres with chitosan and alginate induces both innate and adaptive immune responses which persist up to 63 days of post immunization through parenteral immunization unlike that of free and FIA adjuvented antigens. These results highlight the role of these microspheres as an adjuvant/antigen carrier in fish.
Assuntos
Imunidade Adaptativa , Antígenos de Bactérias/imunologia , Vacinas Bacterianas/imunologia , Edwardsiella tarda/imunologia , Peixes/imunologia , Imunidade Inata , Poliésteres/química , Adjuvantes Imunológicos/farmacologia , Adsorção , Alginatos/metabolismo , Animais , Anticorpos Antibacterianos/sangue , Antígenos de Superfície/imunologia , Quitosana/metabolismo , Doenças dos Peixes/imunologia , Doenças dos Peixes/prevenção & controle , Ácido Glucurônico/metabolismo , Ácidos Hexurônicos/metabolismo , Imunização/métodos , Microesferas , Tamanho da PartículaRESUMO
Calcium phosphate nanoparticles as an antigen/protein delivery was explored in a fish model Labeo rohita H. S-layer protein (of Aeromonas hydrophila) adsorbed on nano sized calcium phosphate particles elicited both innate and adaptive immune parameters which persisted up to 63 days of post immunization through parenteral immunization and gave cross protections.
