RESUMO
Ehrlichia ruminantium, a tick-transmitted pathogen, is the causative agent of heartwater in ruminants. In this study, a proteomic approach was used to identify host cell-specific E. ruminantium proteins encoded by the map1 multigene family, expressed in vitro in bovine endothelial and tick cell cultures. Two-dimensional gel electrophoresis combined with mass spectrometry analysis was used to establish the identities of immunodominant proteins. Proteins extracted from E. ruminantium-infected endothelial cells were shown to be products of the map1 gene, whereas tick cell-derived E. ruminantium proteins were products of a different gene, map1-1. The expressed proteins were found to be glycosylated. Differential expression of MAP1 family proteins in vitro in mammalian and tick cell cultures indicates that the map1 multigene family might be involved in the adaptation of E. ruminantium to the mammalian host and vector tick.
Assuntos
Proteínas de Bactérias/genética , Doenças dos Bovinos/microbiologia , Ehrlichia ruminantium/fisiologia , Regulação Bacteriana da Expressão Gênica/fisiologia , Hidropericárdio/microbiologia , Peptídeos/genética , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/metabolismo , Proteínas de Bactérias/biossíntese , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Bovinos , Células Cultivadas , Ehrlichia ruminantium/genética , Células Endoteliais/citologia , Células Endoteliais/microbiologia , Glicosilação , Interações Hospedeiro-Patógeno/fisiologia , Epitopos Imunodominantes/biossíntese , Epitopos Imunodominantes/química , Epitopos Imunodominantes/genética , Ixodidae/citologia , Ixodidae/microbiologia , Peptídeos/metabolismo , Proteômica , Proteínas Recombinantes/biossíntese , Alinhamento de Sequência , OvinosRESUMO
The prevalence of Babesia infections in domestic dogs in South Africa was studied using reverse line blot hybridization and 18S sequence analysis. Babesia canis vogeli was confirmed for the first time in domestic dogs in South Africa. Out of a total of 297 blood samples collected from domestic dogs in Bloemfontein, East London, Johannesburg, Durban and from the Onderstepoort Veterinary Academic Hospital, 31 were positive for Babesia canis rossi, whereas B. c. vogeli was detected in 13 dogs. None of the dogs carried both parasites. The detection of B. c. vogeli has implications with regard to prevalence and varied clinical manifestation of canine babesiosis in South Africa.
