RESUMO
The aim of this study was to assess the prevalence and risk factors for faecal carriage of extended-spectrum ß-lactamase (ESBL) and plasmidic AmpC ß-lactamase (pAmpC) Escherichia coli producers in dogs. A three-month cross-sectional study was conducted and 151 rectal swabs were obtained from healthy dogs. ESBL and pAmpC genes were detected by PCR and were sequenced. Logistic regression models were used to investigate risk factors for the carriage of ESBL and pAmpC-producing E. coli. About 15 per cent of the isolates carried ESBL genes (blaCTX-M-32 n=8, blaCTX-M-15 n=5, blaCTX-M-1 n=3, blaCTX-M-9-like n=4) and 20 per cent carried pAmpC genes (blaCMY-2 n=23, blaCMY-2-like n=2). Thirteen dogs carried an E. coli isolate with both an ESBL and a pAmpC gene. One E. coli isolate harboured the human blaDHA-1 pAmpC gene, which has not been previously reported in companion animals in Europe. Dogs with a history of antimicrobial therapy in the past year had a higher risk of being carriers of ESBL-producing (P=0.003, ORâ =7.85) and pAmpC-producing (P=0.005, OR=6.28) E. coli. Dogs from shelter/breeders were approximately three times more likely to have an ESBL- or a pAmpC-producing E. coli than dogs from private owners. Males have a reduced risk of carrying a pAmpC-producing E. coli than females (P=0.017, ORâ =0.28). The knowledge of potential risk factors may help to limit the impact of resistance through implementation of effective control measures and judicious antimicrobial therapy.
Assuntos
Proteínas de Bactérias/biossíntese , Portador Sadio/veterinária , Doenças do Cão/microbiologia , Infecções por Escherichia coli/veterinária , Escherichia coli/isolamento & purificação , Escherichia coli/metabolismo , Fezes/microbiologia , beta-Lactamases/biossíntese , Animais , Proteínas de Bactérias/efeitos dos fármacos , Proteínas de Bactérias/genética , Estudos Transversais , Cães , Farmacorresistência Bacteriana , Infecções por Escherichia coli/microbiologia , Feminino , Masculino , Plasmídeos/genética , Fatores de Risco , beta-Lactamases/efeitos dos fármacos , beta-Lactamases/genéticaAssuntos
Infecções por Escherichia coli/microbiologia , Infecções por Escherichia coli/veterinária , Escherichia coli/classificação , Escherichia coli/genética , Variação Genética , Tipagem Molecular , Animais , Análise por Conglomerados , Farmacorresistência Bacteriana , Eletroforese em Gel de Campo Pulsado , Escherichia coli/efeitos dos fármacos , Escherichia coli/isolamento & purificação , Europa (Continente) , Fluoroquinolonas/farmacologia , Genótipo , Humanos , Tipagem de Sequências Multilocus , Animais de Estimação , beta-Lactamases/genética , beta-Lactamas/farmacologiaRESUMO
During 2011, 160 nasal samples were taken from pigs on 8 different farms in Lithuania. Four methicillin-resistant Staphylococcus aureus (MRSA) isolates were obtained. The isolates were ST398, spa type t011 and SCCmec V and none carried the lukF/lukS genes. Strains were resistant to tetracycline, attributed to tetK and tetM genes, and to erythromycin owing to the ermB gene. One MRSA strain was resistant to trimethoprim/sulfamethoxazole and carried the dfrK gene. This is the first report on the presence and characteristics of livestock-associated MRSA isolated from pigs in Lithuania.
