[Genetics and phenogenetics of hormonal characteristics of animals. VIII. Analysis of the variability of the corticosteroid function of the adrenal cortex of silver foxes during their domestication]. / Genetika i fenogenetika gormonal'nykh kharakteristik zhivotnykh. Soobshchenie VIII. Analiz izmenchivosti kortikosteroidnoi funktsii kory nadpochechnikov u serebristo-chernykh lisits v protsesse ikh domestikatsii.

The contribution of genetic and environmental components in phenotype variety of corticosteroid adrenal function was studied in undomesticated and domesticated silver foxes during postnatal ontogenesis. The variation of basal and stress plasma corticosteroid level in animals aged 2, 4, 6, 8 months and in vitro secretion of the adrenal gland at the age 8 months was analysed. Significant genotype-depended variability was only demonstrated in undomesticated foxes under stress stimulation conditions. This phenomenon is manifested from the fourth month of life. However, significant genotype-depended variability was already revealed under basal conditions in domesticated foxes, on initial steps of postnatal ontogenesis. The peculiarities of genetic variability of adrenocortical function in foxes selected for domestication are discussed.

[Effect of t12 haplotype on the manifestation and inheritance of fused and kinky genes in house mice]. / Vliianie gaplotipa t12 na proiavlenie i nasledovanie genov fused i kinky u domovoi myshi.

The influence of some t-haplotypes on the phenotypic manifestation of fused and kinky genes located on chromosome 17 of the house mouse was studied. It was shown that t12-haplotype decreases the penetrance of these genes to 59-70%. The effect was observed when the Fu gene (or Ki) is transmitted from the females heterozygous for t12-haplotype. This haplotype only affects manifestation of the Fu and Ki genes in the F1.

[Genetics and evolution of the Lpm-system in American mink. VI. Identification, genetic control and phylogenesis of the new allotype Lpm13]. / Genetika i évoliutsiia Lpm-sistemy amerikanskoi norki. Soobshchenie VI. Identifikatsiia, geneticheskii kontrol' i filogenez novogo allotipa Lpm13.

Data on immuno- and biochemical identification, genetic control and phylogenesis of new allotype Lpm13 of the Lpm system in domestic mink are presented. This allotype is encountered in mink populations with the frequency 0.9 and higher. The availability of Lpm13 genetic marker permitted another haplotype to be revealed, in addition to the eight known Lpm haplotypes by means of genetic analysis. It was established that, alongside with the earlier described haplotype Lpm3,4,6,8,9,10,11 (abbreviation H3), there exists a similar haplotype, Lpm3,4,6,8,9,10,11,13 (abbreviation H3.13), containing the Lpm13 gene. Of the rest seven haplotypes, five have the Lpm13 gene and two do not. Taking into account this gene and corresponding antigenic marker, the differentiation of 28, instead of 25, phenotypes and 45, instead of 36, genotypes for the Lpm system became possible. Lpm13 antigenic specificity was found with no exception in all individual serum samples taken from ten species and interspecific hybrids of Mustelidae which are closely related to domestic mink. The data obtained give grounds to refer the newly identified Lpm13 gene to the first evolutionary conservative category of genes of the multigenic Lpm system which is also represented by the Lpm6, Lpm9, Lpm10 and Lpm11 genes. The hypotheses of instantaneous formation of polymorphism of the Lpm system in domestic mink are briefly regarded.

[Chromosome segregation in mice heterozygous for Robertsonian translocations. II. Changes in the structure of homologs as a cause of abnormal disjunction in females]. / Segregatsiia khromosom u myshei, geterozigotnykh po robertsonovskim translokatsiiam. Soobshchenie II. Izmenenie struktury gomologov kak prichina anomal'nogo raskhozhdeniia u samok.

It was demonstrated that mutations T, Fu, Ki, t6 of chromosome 17 cause preferential transmission of the acrocentric homologues to the progeny from female Rb heterozygotes. The results indicate that the effects of these mutations on segregation are restricted to the Robertsonian translocations involving chromosome 17. Substitution of the parts of chromosome 17 distal or proximal to the T-locus did not alter the effect, of this chromosome on the transmission rate of the homologue. The transmissions effects of these mutations, whether cis or trans with Rb, were the same. It was observed that mothers Rb7/T43H transmitted the chromosome with the reciprocal translocation T43H to 70.9% of their progeny. Data were obtained supporting the idea that structural changes of the chromosomes caused by mutations affect segregation of the homologues in Rb heterozygous females. The possible mechanism of this influence is discussed.

[Chromosome segregation in mice heterozygous for Robertsonian translocations. I. The effect of mutation in chromosome 17 on the disjunction of homologs in females]. / Segregatsiia khromosom u myshei, geterozigotnykh po robertsonovskim translokatsiiam. Soobshchenie I. Vliianie mutatsii 17-i khromosomy n raskhozhdenie gomologov u samok.

The effects of mutations on chromosome 17 upon the segregation of the metacentric and acrocentric homologues in the progeny of female mice heterozygous for Robertsonian translocations Rb(8.17) 1Iem and Rb(16.17) 7Bnr were studied. Genetic analysis indicated that the ratio of Rb to non-Rb (normal karyotype) progeny from mothers heterozygous for mutations tf, qk, t12 were not significantly different from 1:1 expected. Introduction of mutations T, Ki, Fu, t6 into the female genotype caused strong distortion of segregation and an increase in the proportion of progeny with normal karyotype (65-70%). From the data on embryonic mortality and cytogenetic observations, it is concluded that distortion of equal transmission arises before MII of meiosis. Consequently, preferential distribution of the metacentric chromosome into the polar body during the first meiotic division is relevant to the segregation distortion observed.

[Relative hypertrophy of the right ventricle in silver foxes selected for domestication behavior]. / Otnositel'naia gipertrofiia prvogo zheludochka serdtsa u serebristo-chernykh lisits, selektsioniruemykh na domestikatsionnoe povedenie.

Silver foxes selected for domestication behavior were found to have relative hypertrophy of the right heart ventricle, which was 21% as enlarged in males and 18% as enlarged in females as compared with non-domesticated animals. It was established by stereological methods that hypertrophy occurs mainly at the expense of an increase in the absolute and relative content of myofibrils in cardiomyocytes, with the absolute total volume of the mitochondria being equal both in domesticated and non-domesticated animals. It was shown by means of dissociated cell counts that in both animal groups, the absolute number of cardiomyocytes and their nuclei in the right ventricles is approximately similar. It is suggested that there is a relationship between right heart ventricle hypertrophy in domesticated foxes and variation in the balance of sympathetic and parasympathetic effects.

[Shift in the allele frequencies of the Ld locus in mink stock infected with Aleutian disease]. / Sdvig chastot allelei Ld-lokusa v stadakh norok, porazhennykh aleutskoi bolezn'iu.

An idea on organization of the Ld-system of low density lipoprotein in the domestic mink as on a "closed" immunogenetic system with two codominant alleles Ld1 and Ld2 was confirmed. Significant differences in frequencies of genes and genotypes of the Ld-system between state farm "populations" unaffected with Aleutian disease and those which were centres of this epizootic were established. The results obtained confirm the assumption made earlier on subvitality of the Ld2 gene.

[Effect of hydrocortisone on the frequency and inheritance of the fused gene in mice]. / Vliianie gidrokortizona na proiavlenie i nasledovanie gena fused u myshei.

The influence of hydrocortisone injections given to males on the penetrance and inheritance of fused gene in mice was studied. The existence of a period sensitive to hydrocortisone action was found. The treatment of males during this interval led to a significant decrease in the proportion of offsprings with fused phenotype. Genetic analysis by means of the closely linked recessive tufted marker permitted to show that the lack of phenotypically fused mice is not due to differential mortality of gametes, zygotes or embryos. The most probable reason for this lack is the decrease in penetrance and partially inherited inactivation of fused gene. Possible mechanisms of the phenomenon found are discussed.

[High-polymeric xenogenic RNA as an antitumor immunity stimulator]. / Vysokopolimernaia ksenogennaia RNK kak stimuliator protivoopukholevogo immuniteta.

To immunize CC57BR mice a suspension of live cells of Krebs-2 ascites tumour was administered intradermally into the tail partially amputated afterwards. The growth of the tumour transplanted intraperitoneally was inhibited by 23% only after twofold immunization. Single immunization with tumour cell incubated with the cattle liver RNA preparation in conjunction with intraperitoneal administration of RNA following tumour transplantation inhibited its growth by 43--53%, while twofold administration by 84--88%. The high polymeric fraction of the preparation enhanced the immunization effect to the same measures the initial overall preparation. The treatment of the preparation with RNAase and partial depolymerization of RNA in the course of isolation resulted in the activity loss. It is concluded that the capacity of the RNA preparation for stimulating antitumour immunity is due to high polymeric fraction of RNA.

[Effect of lighting schedules on the ontogeny of gonad and adrenal endocrine function in female silver-black foxes Vulpes vulpes with two genetically determined types of behavior]. / Vliianie svetovykh rezhimov na ontogenez éndokrinnoi funktsii gonad i nadpochechnikov u samok serebristo-chernykh lisits Vulpes vulpes dvukh geneticheski determinirovannykh tipov povedeniia.

Additional illumination of animals nonselected for domesticative behaviour, results in the earlier increase of estradiol level in the blood and in higher production of this hormone by the gonads during pubertal period. Artificial illumination regimes do not significantly affect estradiol-producing activity of the ovaries in animals selected for domesticated behaviour. At the same time, additional illumination of the females with this type of behaviour increased progesteron-producing function of the adrenals during sexual maturation. Thus, selection of silver foxes for the domesticated type of behaviour affects the sensitivity of hormonal function of the ovaries and adrenals to photoperiodic factor of the environment.

[Genetics and phenogenetics of the hormonal characteristics of animals. VI. Functional activity of certain chemoreceptors connected with the pituitary-adrenal system of silver foxes selected for their behavior]. / Genetika i fenogenetika gormonal'nykh kharakteristik zhivotnykh. Soobshchenie VI. Funktsional'naia aktivnost' nekotorykh khemoretseptorov, sviazannykh s gipofizarno-nadpochechnikovoi sistemoi u serebristo-chernykh lisits, selektiruemykh po povedeniiu.

Seasonal differences in the reaction of the pituitary-adrenal system in domesticated and non-domesticated silver foxes of both sexes to substances activating alpha-, beta-adrenoreceptors, and serotonin receptors were studied. It was shown that the reactivity of the pituitary-adrenal system in silver foxes of either type of behaviour is due, at least partially, to seasonal differences in the state of adrenergic and serotoninergic mechanisms. At the same time, in silver foxes selected for behaviour to man the reaction of the pituitary-adrenal system to the injection of substances activating adrenergic and serotoninergic receptors differs, during the year, from the reaction to these compounds in non-selected animals. The conclusion was made, that in the process of domestication changes take place in the state of serotonin- and noradrenaline mechanisms connected with the regulation of the hypothalamo-pituitary-adrenal complex.

[Problems in the genetics of stress. IV. A genetic analysis of the level of autonomic reactivity in emotional stress in rats]. / Problemy genetiki stressa. Soobshchenie IV. Geneticheskii analiz urovnia vegetativnoi reaktivnosti pri émotsional'nom stresse u krys.

A genetic analysis of hypertensive reactions under condition of emotional stress in three inbred strains of rats (Wistar, Sprague-Dowly and August) and their F1 reciprocal hybrids is carried out. Highly significant genotypic differences in the arterial pressure level during emotional stress and no genetic diversity in the basal state were found. Application of our own methos to genetic analysis of incomplete diallelic tables for Griffing's experimental design 1 (model 1) showed an existence of significant additive component of genetic variance in autonomic reactivity under the stress. The role of the stress as a factor of differentiation of the genetic structure of population is discussed.

[Genetic control over 7 Lpm-system allotypes of very high-density serum alpha2-lipoprotein the mink]. / Geneticheskii kontrol' semi allotipov Lpm-sistemy al'fa2-lipoproteina ochen' vysokoi plotnosti syvorotki krovi norki.

Population and hybridological analyses have demonstrated that the alloantigenic markers of very high density alpha2-lipoprotein of mink serum, Lpm7 and Lpm8, along with Lpm1, Lpm2, Lpm3, Lpm4, Lpm5, belong to a common immunogenetic system. 21 Lpm-phenotypes are determined at least by 36 genotypes; each of the 11 phenotypes is conditioned by a single genotype, each of the remaining 10 are conditioned by 2, 3 or 4 genotypes. The Lpm allotypes and allogroups are coded by the genetic units Lpm8, Lpm4, Lpm4,8, Lpm4,7, Lpm3,4,8, Lpm1,8, Lpm1,2,7, Lpm2,4,5,7, which behave as alleles. The last, six seem to be haploid sets of closely linked genes.

[Further genetic analysis of mink serum Lpm-allotypes]. / Dal'neishii geneticheskii analiz LPM-allotipov syvorotki norok.

The results obtained by testing 3127 mink sera as well as the analysis of families completely confirmed that the Lpm allotype system of very high density proteins is under genetic control, as postulated earlier. The polymorphism of the first five allotypes is due to the genetic units Lpm1, Lpm4, Lpm1,2, Lpm3,4 and Lpm2,4,5, which behave as Mendelian alleles. The data obtained suggest the existence of an additional gene, LpmX, which, in the homozygous state, determines a very rare phenotype, negative for the first five markers.