[Stem cell transplantation in Morocco: report of 87 cases]. / Greffe de cellules souches hématopoïétiques au Maroc : à propos de 87 cas.

PURPOSE: The autologous peripheral blood stem cells (CSP) are used as a support to the restoration of the hematopoiesis in oncohematology. The objective of this work, carried out in the laboratory of cryobiology at the Center Regional of Blood Transfusion, Casablanca, is to assess the short- and long-term results of this type of autograft. PATIENTS AND METHODS: This retrospective study focused on 87 patients treated for hematological malignancies in services of oncohaematology over a period from June 2004 to March 2009. One hundred and twenty-seven cytapheresis were conducted to collect the CSP. The rate of CD34+ cells ranged from two to 50x10(6) per kilogram. The removal, treatment with 10% DMSO, the characterization, cryopreservation, thawing and washing of grafts on Cobe 2991 were made before the autograft. RESULTS: Forty-nine patients were transplanted for multiple myeloma, 24 for Hodgkin's disease, 13 for a non-Hodgkin lymphoma and one for rabdomyosarcome. The mean age of patients was 35 years. The mean length of hospitalization was 27 days. The average time of release for aplasia is 7 to 49 days, with a mean of 16 days. A blood transfusion was necessary during the aplasia: two to 41 red blood cells per patient and six to 298 platelet units per patient. The short-term complications after autologous transplantation are primarily infectious. Seven times the progressive hematologic malignancies and seven deaths are regrettable. CONCLUSION: The autologous CSP is simple, effective and well tolerated, which should be developed in our context.

[Kluyvera bacteriemia: an epidemic in a Moroccan teaching hospital]. / Bactériémies à Kluyvera: à propos d'une épidémie dans un CHU au Maroc.

Kluyvera Spp is an enterobacterium rarely isolated in medical microbiology, an opportunistic pathogen the clinical significance of which remains unclear. Four strains of Kluyvera Spp were isolated for the first time in the Ibn Rochd Teaching Hospital microbiology laboratory. These strains were isolated in hemocultures of four patients hospitalized in the same medical ward, at the same time, and presenting with bacteriemia. An antibiotic susceptibility study allowed identifying the wild phenotype in the first isolate, the three next isolates had acquired resistance to trimethoprim-sulfamethoxazole, gentamycin, tobramycin, and ciprofloxacin, and produced broad-spectrum betalactamase. The epidemiological investigation in the patients' environment made after isolating the fourth strain was negative. The four patients evolved uneventfully without antibiotic treatment. This raises the question of the real pathogenic capacity of these strains, their epidemic power, and their ability to acquire resistance.