The Non-Dereliction in Evolution: Trophic Specialisation Drives Convergence in the Radiation of Red Devil Spiders (Araneae: Dysderidae) in the Canary Islands.

Natural selection plays a key role in deterministic evolution, as clearly illustrated by the multiple cases of repeated evolution of ecomorphological characters observed in adaptive radiations. Unlike most spiders, Dysdera species display a high variability of cheliceral morphologies, which has been suggested to reflect different levels of specialization to feed on isopods. In this study, we integrate geometric morphometrics and experimental trials with a fully resolved phylogeny of the highly diverse endemic species from the Canary Islands to 1) quantitatively delimit the different cheliceral morphotypes present in the archipelago, 2) test their association with trophic specialization, as reported for continental species, 3) reconstruct the evolution of these ecomorphs throughout the diversification of the group, 4) test the hypothesis of convergent evolution of the different morphotypes, and 5) examine whether specialization constitutes a case of evolutionary irreversibility in this group. We show the existence of 9 cheliceral morphotypes and uncovered their significance for trophic ecology. Further, we demonstrate that similar ecomorphs evolved multiple times in the archipelago, providing a novel study system to explain how convergent evolution and irreversibility due to specialization may be combined to shape phenotypic diversification in adaptive radiations.

The Vitruvian spider: Segmenting and integrating over different body parts to describe ecophenotypic variation.

Understanding what drives the existing phenotypic variability has been a major topic of interest for biologists for generations. However, the study of the phenotype may not be straightforward. Indeed, organisms may be interpreted as composite objects, comprising different ecophenotypic traits, which are neither necessarily independent from each other nor do they respond to the same evolutionary pressures. For this reason, a deep biological understanding of the focal organism is essential for any morphological analysis. The spider genus Dysdera provides a particularly well-suited system for setting up protocols for morphological analyses that encompass a suit of morphological structures in any nonmodel system. This genus has undergone a remarkable diversification in the Canary Islands, where different species perform different ecological roles, exhibiting different levels of trophic specialization or troglomorphic adaptations, which translate into a remarkable interspecific morphological variability. Here, we seek to develop a broad guide, of which morphological characters must be considered, to study the effect of different ecological pressures in spiders and propose a general workflow that will be useful whenever researchers set out to investigate variation in the body plans of different organisms, with data sets comprising a set of morphological traits. We use geometric morphometric methods to quantify variation in different body structures, all of them with diverse phenotypic modifications in their chelicera, prosoma, and legs. We explore the effect of analyzing different combined landmark (LM) configurations of these characters and the degree of morphological integration that they exhibit. Our results suggest that different LM configurations of each of these body parts exhibit a higher degree of integration compared to LM configurations from different structures and that the analysis of each of these body parts captures different aspects of morphological variation, potentially related to different ecological factors.