1.
Biotechniques
; 28(4): 776-83, 2000 Apr.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10769757
RESUMO
Gene expression profiling using an AFLP-based technique generates a large number of gene fragments that require identification by sequencing. The DNA fragments vary in length from about 50-500 bp. Ion-pair reversed-phase HPLC can be used to purify selected double-stranded DNA fragments that represent differentially expressed genes. The gene fragments are sequenced directly after vacuum drying of the collected HPLC fractions.