Spectral Photoluminescent Parameters of Barley Seeds (Hordéum vulgáre) Infected with Fusarium ssp.

We needed effective and sustainable technologies for better microbiological control of crops, including Fusarium. However, photoluminescent UV-Vis methods are potential for diagnosing plant diseases with Fusarium. It has not been sufficiently studied despite the application of these methods for other biological researches. The excitation spectrum of the seeds during infection shifts to the shorter wavelength and a new maximum appears in the region λ ≈ 232 nm. The photoluminescence of infected seeds increases with excitation by radiation of wavelengths λe,1 = 232 nm, λe,2 = 362 nm and λe,3 = 424 nm by 1.33-3.14 times, and λe,3 = 424 nm-decreases by 1.1 times. Statistical moments µ3 and µ4 , asymmetry and kurtosis change only with short-wave excitation. When analyzing the decomposition of the frequency spectrum into Gaussian curves, the most informative ratio is the ratio of right-handed and left-handed Gaussians under excitation λe,2 = 362 nm and λe,3 = 424 nm. The ratios of their maxima change during infection by 1.36-3.2 times, and for excitation by radiation λe,2 , the frequency boundaries of Gaussians change. The results of measurements and calculations provide a basis for the development of a method and device for photoluminescence diagnostics of fusarium seeds in UV-Vis ranges.

Detection of Fusarium infected seeds of cereal plants by the fluorescence method.

Infection of seeds of cereal plants with fusarium affects their optical luminescent properties. The spectral characteristics of excitation (absorption) in the range of 180-700 nm of healthy and infected seeds of wheat, barley and oats were measured. The greatest difference in the excitation spectra of healthy and infected seeds was observed in the short-wave range of 220-450 nm. At the same time, the excitation characteristics of infected seeds were higher than those of healthy ones, and the integral parameter Η in the entire range was 10-56% higher. A new maximum appeared at the wavelength of 232 nm and the maximum value increased by 362 nm. The spectral characteristics were measured when excited by radiation at wavelengths of 232, 362, 424, 485, 528 nm and the luminescence fluxes were calculated. It is established that the photoluminescence fluxes Φ in the short-wave ranges of 290-380 nm increase by 1.58-3.14 times and 390-550 nm-by 1.44-2.54 times. The fluxes in longer wavelength ranges do not change systematically and less significantly: for wheat, they decrease by 12% and increase by 19%, for barley, they decrease by 10% and increase by 33%. The flux decreases by 43-71% for oats. Based on the results obtained for cereal seeds, it is possible to further develop a method for detecting fusarium infection with absolute measurements of photoluminescence fluxes in the range of 290-380 nm, or when measuring photoluminescence ratios: for wheat seeds when excited with wavelengths of 424 nm and 232 nm (Φ424/Φ232); for barley seeds-when excited with wavelengths of 485 nm and 232 nm (Φ485/Φ232) and for oat seeds-when excited with wavelengths of 424 nm and 362 nm (Φ424/Φ362).

Photoluminescent Sensor of Scarification Efficiency of Fodder Plants' Seeds.

Optoelectronic sensors open up new possibilities for predicting the yield for their possible correction, including increasing the seed germination of forage plants. The luminescent properties of unscarified and scarified seeds of various germination galega, clover and alfalfa are compared. The dependence of germination on the photoluminescence flux is approximated by linear equations with a determination coefficient R2 = 0.932-0.999. A technological process for analyzing the scarification quality of forage seed plants is proposed, including sample preparation, photoluminescence excitation and registration, amplification of the received electrical signal and determination of germination based on calibration equations. This is followed by a decision on sowing, or re-scarification. The scheme of the scarification quality control device has been developed for which the LED, as well as the radiation receiver and other elements, has been selected according to the energy efficiency criterion. Mechanical scarification of the forage plants' seed surfaces has a significant effect on their photoluminescent properties. The flux increases by 1.5-1.7 times for galega, 2.0-3.0 times for clover and 2.3-3.9 times for alfalfa. Linear approximation of the flux dependence on germination with a high coefficient of determination allows us to obtain reliable linear calibration equations. Preliminary mock-up laboratory tests allow us to talk about the developed method's effectiveness and device.

Upregulation of NETO2 gene in colorectal cancer.

BACKGROUND: Neuropilin and tolloid-like 2 (NETO2) is a single-pass transmembrane protein that has been shown primarily implicated in neuron-specific processes. Upregulation of NETO2 gene was also detected in several cancer types. In colorectal cancer (CRC), it was associated with tumor progression, invasion, and metastasis, and seems to be involved in epithelial-mesenchymal transition (EMT). However, the mechanism of NETO2 action is still poorly understood. RESULTS: We have revealed significant increase in the expression of NETO2 gene and deregulation of eight EMT-related genes in CRC. Four of them were upregulated (TWIST1, SNAIL1, LEF1, and FOXA2); the mRNA levels of other genes (FOXA1, BMP2, BMP5, and SMAD7) were decreased. Expression of NETO2 gene was weakly correlated with that of genes involved in the EMT process. CONCLUSIONS: We found considerable NETO2 upregulation, but no significant correlation between the expression of NETO2 and EMT-related genes in CRC. Thus, NETO2 may be involved in CRC progression, but is not directly associated with EMT.