[Relationship between left ventricular diastolic dysfunction and coronary disease in type 2 diabetes mellitus]. / Relation entre la dysfonction diastolique du ventricule gauche et la présence d'atteintes coronaires chez le sujet diabétique type 2.

INTRODUCTION: Type 2 diabetes is associated with an increased risk of coronary disease and is the leading cause of morbidity and mortality in this population. The main objective of our work is to study the correlation of diastolic function of the left ventricle with coronary disease in type 2 diabetics. MATERIAL AND METHODS: Analytical cross-sectional, monocentric prospective-looking study of 703 type 2 diabetic patients performed at the Military Regional Hospital of Constantine over a period of 04 years (2016-2019). We excluded 338 patients who did not receive coronary angiography; thus 365 patients are ultimately analyzed. Evaluation of diastolic function was performed by two-dimensional transthoracic echocardiography with the search of coronary disease. The data was analyzed using the Epi Info 7.2.1.0 with study of the relationship of the diastolic function to coronary disease by multiple logistic regression. RESULTS: The average age of our final cohort is 57,7±6,5 years, an average of 7.4±1.8% of glycated hemoglobin, an average of 5,8±4,1 years of diabetes, a sex ratio to 1.27. 49.3% had diastolic dysfunction. The prevalence of coronary disease is 32,9%. In multivariate analysis; diastolic dysfunction is correlated with coronary involvement significantly (OR=2.02, 95% CI [1.50 - 2.90], p=0.02). CONCLUSION: The prevalence of diastolic dysfunction is high in type 2 diabetics and is significantly correlated with coronary heart disease.

In-house preparation and evaluation of (125)I-histamine progesterone tracer for radioimmunoassay of progesterone.

The progesterone tracer for the development of in-house radioimmunoassay was prepared in two steps clearly described. First, activation of 11α-hemisuccinate progesterone (progesterone-11 α HS) and labeling of histamine with (125)I, and second, conjugation of activated 11α-HS progesterone with iodinated histamine. The purification of the tracer was carried out by gel filtration on Sephadex G-25. The radiochemical purity of purified the tracer was 95%. The maximum binding using solid phase (coated tubes) reached 41% and the non specific binding didn't exceed 5%. The tracer stored at different temperatures 10°C and -6°C was stable during 12 weeks. The skim milk provided assay better sensitivity (0.22 ng/mL) than serum (0.28 ng/mL).