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1.
Curr Med Chem ; 13(15): 1749-55, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-16787218

RESUMO

Reduced glutathione (GSH) is present in millimolar concentrations in mammalian cells. It is involved in many cellular functions such as detoxification, amino acid transport, production of coenzymes, and the recycling of vitamins E and C. GSH acts as a redox buffer to preserve the reduced intracellular environment. Decreased glutathione levels have been found in numerous diseases such as cancer, viral infections, and immune dysfunctions. Many antioxidant molecules, such as GSH and N-acetylcysteine (NAC), have been demonstrated to inhibit in vitro and in vivo viral replication through different mechanisms of action. Accumulating evidence suggests that intracellular GSH levels in antigen-presenting cells such as macrophages, influence the Th1/Th2 cytokine response pattern, and more precisely, GSH depletion inhibits Th1-associated cytokine production and/or favours Th2 associated responses. It is known that GSH is not transported to most cells and tissues in a free form. Therefore, a number of different approaches have been developed in the last years to circumvent this problem. This review discusses the capacity of some new molecules with potent pro-GSH effects either to exert significant antiviral activity or to augment GSH intracellular content in macrophages to generate and maintain the appropriate Th1/Th2 balance. The observations reported herein show that pro-GSH molecules represent new therapeutic agents to treat antiviral infections and Th2-mediated diseases such as allergic disorders and AIDS.


Assuntos
Adjuvantes Imunológicos/farmacologia , Antivirais/farmacologia , Glutationa/farmacologia , Animais , Glutationa/fisiologia , Humanos , Camundongos , Células Th1/imunologia , Células Th2/imunologia , Viroses/fisiopatologia
2.
J Prev Med Hyg ; 47(4): 134-7, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17263158

RESUMO

From October 2004 to September 2005, Radon222 activity in high-risk indoor spaces used by employees and students at the University of Genoa was measured with CR-39 nuclear track detectors. The mean concentration in winter (78.9 Bq/m3 +/- 74.92 S.D.) was low in relation to the microenvironment considered. When data were broken down by type and location of the spaces, no significant differences were found, despite the fact that the Genoa conurbation lies on soil of variable geological composition. The dose absorbed by employees was 0.42 mSv/year, with a relative risk of 4.2/1000 cases of Radon-related lung cancer. The dose absorbed by students was 0.28 mSv/year, with a relative risk of 2.5/1000 cases of Radon-related lung cancer. The level of radon activity detected never exceeded the limit of 500 Bq/m3 established by Italian law. Nevertheless, the value of the compound uncertainty index suggested that the real level of Radon contamination could have exceeded 400 Bq/m3 in selected spaces, a value requiring annual concentration tests.


Assuntos
Poluentes Radioativos do Ar/análise , Poluição do Ar em Ambientes Fechados/análise , Exposição Ambiental/prevenção & controle , Lesões por Radiação/prevenção & controle , Monitoramento de Radiação , Radônio/análise , Sistema Respiratório/efeitos da radiação , Universidades , Poluentes Radioativos do Ar/toxicidade , Poluição do Ar em Ambientes Fechados/efeitos adversos , Poluição do Ar em Ambientes Fechados/prevenção & controle , Brônquios/efeitos da radiação , Exposição Ambiental/estatística & dados numéricos , Epitélio/efeitos da radiação , Habitação/normas , Humanos , Itália/epidemiologia , Alvéolos Pulmonares/efeitos da radiação , Lesões por Radiação/epidemiologia , Radônio/toxicidade , Medição de Risco , Estações do Ano , Local de Trabalho
4.
Neurochem Int ; 41(1): 55-63, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-11918972

RESUMO

Prion diseases are fatal neurodegenerative disorders of the CNS of men and animals, characterized by spongiform degeneration of the CNS, astrogliosis and deposition of amyloid into the brain. The conversion of a cellular glycoprotein (the prion protein, PrP(C)) into an altered isoform (the prion scrapie, PrP(Sc)), which accumulates within the brain tissue by virtue of its resistance to the intracellular catabolism, is currently believed to represent the etiologic agent responsible for these diseases. Synthetic or recombinant polypeptides are commonly used to elucidate the mechanism of proteins involved in neurodegenerative diseases. Here we describe a procedure, which allows the synthesis and purification in its native folding, of the human prion protein fragment 90-231, corresponding to the protease resistant core of PrP(Sc). We synthesized the polypeptides 90-231 of both the wild type and the E200K mutant isoforms of PrP. Using a gluthatione S-transferase (GST) fusion protein approach, milligram amounts of polypeptides were obtained after expression in E. coli. The recovery of the purified fusion protein was monitored following the evaluation of the GST activity. The PrP fragment was released from the fusion protein immobilized on a glutathione-coupled agarose resin by direct cleavage with thrombin. The recombinant protein was identified by comassie stained acrylamide gel and by immunoblotting employing a monoclonal anti-PrP antibody. The peptide purified by gel filtration chromatography showed mainly an alpha-helix structure, as analysed by circular dichroism (CD) and an intact disulfide bridge. The same procedure was also successfully employed to synthesize and purify the E200K mutant PrP fragment.


Assuntos
Escherichia coli/genética , Príons/genética , Sequência de Bases , Western Blotting , Cromatografia Líquida , Dicroísmo Circular , Primers do DNA , Humanos , Espectrometria de Massas , Príons/isolamento & purificação , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Espectrometria de Fluorescência
5.
Biotechnol Appl Biochem ; 34(3): 151-9, 2001 12.
Artigo em Inglês | MEDLINE | ID: mdl-11730482

RESUMO

Many different unique functions have been attributed to lactoferrin (Lf), including DNA and RNA binding, and transport into the nucleus, where Lf binds to specific sequences and activates transcription. A pentapeptide, Gly-Arg-Arg-Arg-Arg, corresponding to a region of the N-terminal portion of human Lf rich in basic amino acids, was synthesized and its intracellular localization was investigated. Peptide internalization was assayed using the rhodaminated form of the same molecule. This N-terminal peptide sequence is able to be internalized within less than 10 min at concentration as low as 1 microM, and its intracellular localization is nuclear, mainly nucleolar. Similar behaviour was observed using peptides composed of either all l or d amino acids, the last one being a retro-inverse peptide. The internalization process does not involve an endocytotic pathway, since no inhibition of the uptake was observed at 4 degrees C. The kinetics of peptide internalization was also evaluated. The internalization properties of such a short Lf pentapeptide have been assayed for its ability to transport peptide nucleic acids (PNAs) inside cells in order to improve their efficacy. The abundant transmembrane transport and nuclear localization of the proposed peptide, deriving from hLf and, for the first time, identified as a nuclear localization signal, could be used as an alternative strategy to tackle the unsolved problem of intracellular accumulation of antisense and antigene drugs and for the development of new pharmacological tools.


Assuntos
Núcleo Celular/metabolismo , Lactoferrina/metabolismo , Sinais de Localização Nuclear/metabolismo , Ácidos Nucleicos Peptídicos/metabolismo , Motivos de Aminoácidos , Nucléolo Celular/metabolismo , Endocitose , Corantes Fluorescentes/química , Humanos , Lactoferrina/química , Mimetismo Molecular , Ácidos Nucleicos Peptídicos/química , Rodaminas/química , Temperatura , Células Tumorais Cultivadas
6.
Nitric Oxide ; 5(5): 427-31, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11587557

RESUMO

The presence of Ca(2+)-dependent, heat-stress-activated nitric oxide synthase (NOS) activity in peculiarly shaped, fusiform, and dendritic sponge cells is described for the first time. The NOS activity was evidenced evaluating the conversion of radioactive citrulline from [(14)C]arginine in intact cells from two different species that are phylogenetically unrelated in the class of Demospongiae: Axinella polypoides and Petrosia ficiformis. The production of nitrogen monoxide (NO) was confirmed by electron paramagnetic resonance analysis, and the histochemistry technique of NADPH diaphorase showed a specific localization of NOS activity in a particular network of dendritic cells in the sponge parenchyma. Sponges are the most primitive metazoan group; their evolution dates back 600 million years. The presence of environmental stress-activated NOS activity in these organisms may prove to be the most ancient NO-dependent signaling network in the animal kingdom.


Assuntos
Cálcio/metabolismo , Óxido Nítrico Sintase/metabolismo , Poríferos/enzimologia , Estresse Fisiológico/metabolismo , Animais , Citrulina/metabolismo , Células Dendríticas/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Temperatura Alta , Técnicas Imunoenzimáticas , NADPH Desidrogenase/metabolismo , Óxido Nítrico/metabolismo
7.
Biotechnol Appl Biochem ; 34(1): 19-23, 2001 08.
Artigo em Inglês | MEDLINE | ID: mdl-11483150

RESUMO

The L-arginine uptake of resting platelets from type-2 diabetic patients and control subjects was measured and the kinetic parameters defined. The effect of platelet stimulation with agonists was also investigated. Kinetic studies showed that the K(m) value for L-arginine transport was not different in patients compared with control subjects, while V(max) was significantly decreased in patients compared with controls. Moreover, agonists able to mobilize Ca(2+) produced a further decrease in the L-arginine uptake in controls and in patients, suggesting that Ca(2+) intracellular levels down-regulate L-arginine transport in platelets from both control subjects and patients. Data suggest that drugs designed to control intracellular Ca(2+) might restore platelet function in diabetic patients.


Assuntos
Arginina/farmacocinética , Plaquetas/metabolismo , Diabetes Mellitus Tipo 2/sangue , Idoso , Calcimicina/farmacologia , Cálcio/metabolismo , Cálcio/farmacologia , Relação Dose-Resposta a Droga , Regulação para Baixo , Feminino , Humanos , Cinética , Masculino , Pessoa de Meia-Idade , Óxido Nítrico/metabolismo , Fatores de Tempo
8.
Nat Biotechnol ; 19(3): 231-4, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11231555

RESUMO

Peptide nucleic acids (PNAs) may be a potent tool for gene function studies in medically important parasitic organisms, especially those that have not before been accessible to molecular genetic knockout approaches. One such organism is Entamoeba histolytica, the causative agent of amebiasis, which infects about 500 million people and is the cause of clinical disease in over 40 million each year, mainly in the tropical and subtropical world. We used PNA antisense oligomers to inhibit expression of an episomally expressed gene (neomycin phosphorotransferase, NPT) and a chromosomal gene (EhErd2, a homolog of Erd2, a marker of the Golgi system in eukaryotic cells) in axenically cultured trophozoites of E. histolytica. Measurement of NPT enzyme activity and EhErd2 protein levels, as well as measurement of cellular proliferation, revealed specific decreases in expression of the target genes, and concomitant inhibition of cell growth, in trophozoites treated with micromolar concentrations of unmodified antisense PNA oligomers.


Assuntos
Elementos Antissenso (Genética)/farmacologia , Regulação para Baixo/efeitos dos fármacos , Entamoeba histolytica/efeitos dos fármacos , Canamicina Quinase/metabolismo , Ácidos Nucleicos Peptídicos/farmacologia , Animais , Elementos Antissenso (Genética)/genética , Biomarcadores/análise , Divisão Celular/efeitos dos fármacos , Entamoeba histolytica/enzimologia , Entamoeba histolytica/genética , Entamoeba histolytica/crescimento & desenvolvimento , Gentamicinas/farmacologia , Complexo de Golgi/efeitos dos fármacos , Complexo de Golgi/metabolismo , Canamicina Quinase/biossíntese , Canamicina Quinase/genética , Proteínas de Membrana/biossíntese , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Microscopia de Fluorescência , Neomicina/metabolismo , Ácidos Nucleicos Peptídicos/genética , Permeabilidade , Transfecção
10.
Antiviral Res ; 47(3): 149-58, 2000 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-10974367

RESUMO

The most common therapies against human herpes virus (HSV-1) and human immunodeficiency virus (HIV-1) infectivity are based on the administration of nucleoside analogues. Acyclovir (ACV) is the drug of choice against HSV-1 infection, while the acyclic nucleoside phosphonate analogue PMPA has shown marked anti-HIV activity in a phase I and II clinical studies. As monocyte-derived macrophages are assumed to be important as reservoirs of both HSV-1 and HIV-1 infection, new approaches able to inhibit replication of both viruses in macrophages should be welcome. ACVpPMPA, a new heterodinucleotide consisting of both an antiherpetic and an antiretroviral drug bound by a phosphate bridge, was synthesized and encapsulated into autologous erythrocytes modified to increase their phagocytosis by human macrophages. ACVpPMPA-loaded erythrocytes provided an effective in vitro protection against both HSV-1 and HIV-1 replication in human macrophages.


Assuntos
Fármacos Anti-HIV/farmacologia , HIV-1/efeitos dos fármacos , Herpesvirus Humano 1/efeitos dos fármacos , Nucleosídeos/farmacologia , Pró-Fármacos/farmacologia , Animais , Fármacos Anti-HIV/metabolismo , Chlorocebus aethiops , Portadores de Fármacos , Eritrócitos/metabolismo , HIV-1/crescimento & desenvolvimento , Herpesvirus Humano 1/crescimento & desenvolvimento , Humanos , Técnicas In Vitro , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Macrófagos/virologia , Nucleosídeos/metabolismo , Pró-Fármacos/metabolismo , Células Vero , Replicação Viral/efeitos dos fármacos
11.
Tissue Antigens ; 55(3): 212-8, 2000 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10777096

RESUMO

Interferons upregulate the expression of HLA class I antigens on cancer cells. Nevertheless, little is known about the panel of HLA class I antigen-associated peptides presented by recombinant alpha-interferon (r(alpha)-IFN)-treated cells. For this reason, peptides were eluted from five cancer cell lines (four melanoma and one non-small cell lung cancer) following treatment with r(alpha)-IFN. High-performance liquid chromatography (HPLC) profiles of the peptide fractions were compared with those obtained from untreated cells. No significant differences in peptide characteristics (detectable on the basis of retention times) were observed, but significant differences in terms of peptide quantities were observed. Mass spectrometry performed on HPLC peaks allowed not only the detection of three different peptides (two derived from the MAGE family of genes and one from the mart-1) both in untreated and in treated cells, but also gave an indication of the number of peptides within one HPLC peak. This data demonstrates that r(alpha)-IFN-treated cells express a similar peptide pattern as untreated cells, with significant quantitative differences. Interestingly, this finding also explains the higher susceptibility to lysis (mediated by specific cytolytic lymphocytes, which recognize cancer cells in an HLA-restricted fashion) of r(alpha)-IFN-treated cells.


Assuntos
Antígenos de Histocompatibilidade Classe I/metabolismo , Interferon-alfa/farmacologia , Peptídeos/metabolismo , Antígenos de Neoplasias/metabolismo , Antígenos HLA-A/metabolismo , Antígenos HLA-B/metabolismo , Humanos , Células K562 , Antígeno MART-1 , Antígenos Específicos de Melanoma , Proteínas de Neoplasias/metabolismo , Células Tumorais Cultivadas
12.
Cancer Res ; 60(8): 2258-62, 2000 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-10786693

RESUMO

Peptide nucleic acids (PNAs) are synthetic structural analogues of DNA and RNA that, if allowed to enter the cell, bind to the complementary polynucleotide sequence and inhibit DNA transcription and mRNA translation. Although PNAs have a very limited ability in penetrating nuclei of living cells, there are indications that covalent linkage of the PNA to appropriate vectors, e.g., a nuclear localization signal, permits access to the genome. Here we test the ability of dihydrotestosterone (T) covalently linked to PNA to act as a vector for targeting c-myc DNA to prostatic cancer cell nuclei. LNCaP cells, which express the androgen receptor gene, and DU145 cells, in which the androgen receptor gene is silent, offer a model to test this biologically active hormone as a cell-specific vector. T vector was covalently linked to the NH2-terminal position of a PNA complementary to a unique sequence of c-myc oncogene (PNAmyc-T). To localize PNAmyc-T and vector-free PNA within the cells, a rhodamine (R) group was attached at the COOH-terminal position (PNAmyc-R, PNAmyc-TR); cellular uptake was monitored by confocal fluorescence microscopy. PNAmyc-R was detected only in the cytoplasm of both prostatic cell lines, whereas PNAmyc-TR was localized in nuclei as well as in cytoplasm of LNCaP cells. In contrast, PNAmyc-TR uptake in DU145 cells was minimal and exclusively cytoplasmic. In LNCaP cells, MYC protein remained unchanged by exposure to vector-free PNAmyc, whereas a significant and persistent decrease was induced by PNAmyc-T. In DU145 cells, MYC expression was unaltered by PNAmyc with or without the T vector. Our data show that the T vector facilitates cell-selective nuclear localization of PNA and its consequent inhibition of c-myc expression. These findings suggest a strategy for targeting of cell-specific anti-gene therapy in prostatic carcinoma.


Assuntos
Núcleo Celular/metabolismo , Di-Hidrotestosterona/metabolismo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Ácidos Nucleicos Peptídicos/metabolismo , Ácidos Nucleicos Peptídicos/farmacologia , Neoplasias da Próstata/metabolismo , Elementos Antissenso (Genética)/genética , Elementos Antissenso (Genética)/metabolismo , Elementos Antissenso (Genética)/farmacocinética , Elementos Antissenso (Genética)/farmacologia , Transporte Biológico , Divisão Celular/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Citoplasma/metabolismo , Genes myc/genética , Terapia Genética , Humanos , Masculino , Microscopia de Fluorescência , Sinais de Localização Nuclear , Ácidos Nucleicos Peptídicos/química , Ácidos Nucleicos Peptídicos/farmacocinética , Neoplasias da Próstata/genética , Neoplasias da Próstata/patologia , Neoplasias da Próstata/terapia , Receptores Androgênicos/deficiência , Receptores Androgênicos/genética , Receptores Androgênicos/metabolismo , Especificidade por Substrato , Células Tumorais Cultivadas
13.
FEBS Lett ; 451(3): 264-8, 1999 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-10371202

RESUMO

Overexpression of inducible nitric oxide synthase causes the production of high levels of nitric oxide, which, under pathological conditions, leads to immunosuppression and tissue damage. The results recently obtained using peptide nucleic acids, rather than traditional oligonucleotides as antigen and antisense molecules, prompted us to test their efficacy in the regulation of nitric oxide production, thereby overcoming the obstacle of cellular internalization. The cellular permeability of four inducible nitric oxide synthase antisense peptide nucleic acids of different lengths was evaluated. These peptide nucleic acids were covalently linked to a hydrophobic peptide moiety to increase internalization and to a tyrosine to allow selective 125I radiolabelling. Internalization experiments showed a 3-25-fold increase in the membrane permeability of the modified peptide nucleic acids with respect to controls. Inducible nitric oxide synthase inhibition experiments on intact stimulated macrophages RAW 264.7 after passive permeation of the two antisense peptide nucleic acids 3 and 4 demonstrated a significant decrease (43-44%) in protein enzymatic activity with respect to the controls. These data offer a basis for developing a good alternative to conventional drugs directed against inducible nitric oxide synthase overexpression.


Assuntos
Macrófagos/metabolismo , Óxido Nítrico Sintase/antagonistas & inibidores , Óxido Nítrico Sintase/metabolismo , Óxido Nítrico/metabolismo , Ácidos Nucleicos Peptídicos/metabolismo , Ácidos Nucleicos Peptídicos/farmacologia , Animais , Elementos Antissenso (Genética)/genética , Elementos Antissenso (Genética)/metabolismo , Elementos Antissenso (Genética)/farmacologia , Transporte Biológico , Linhagem Celular , Camundongos , Óxido Nítrico Sintase Tipo II , Ácidos Nucleicos Peptídicos/genética
14.
J Acquir Immune Defic Syndr ; 21(2): 81-9, 1999 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-10360798

RESUMO

Macrophages play a key role in AIDS pathogenesis and thus controlling infectivity and viral replication in these cells is a key issue in any antiretroviral therapy. In the present study, using a murine model of AIDS, we evaluated new therapeutic approaches specifically designed for the protection of macrophages. Based on previous observations, we took advantage of the unique ability of autologous erythrocytes to deliver drugs selectively to macrophages. The antiviral drugs selected were a new homodimer of AZT (AZTp2AZT) and reduced glutathione (GSH). The addition of an oral drug for the protection of lymphocytes (i.e., AZT) was also investigated. C57BL/6 mice infected with the retroviral complex LP-BM5 were treated with GSH-loaded erythrocytes, GSH-loaded erythrocytes plus oral AZT, or GSH/AZTp2AZT-loaded erythrocytes plus oral AZT. The treatments including AZT and erythrocytes loaded with GSH alone or with GSH plus AZTp2AZT provided similar results and were most effective in inhibiting the progression of MAIDS; they reduced splenomegaly, lymphadenopathy, and hypergammaglobulinemia by about 70%, 90% and 83%, respectively, when compared with infected animals at 10 weeks postinfection. Evaluation of BM5d proviral DNA content in infected organs revealed that both treatments were able to almost completely protect most infected animals. They were also able to normalize the blood lymphocyte phenotype and to restore the responses of T and B cells to mitogens significantly. Treatment with GSH-loaded erythrocytes alone did not provide significant results for most parameters investigated, but a marked reduction in proviral DNA content was obtained in infected organs, including the brain. The results reported in this paper confirm the important role of macrophages in retroviral infection and moreover prove that erythrocytes, by selectively protecting these cells, strongly affect MAIDS progression. Furthermore, the combination of GSH- or GSH/AZTp2AZT-loaded erythrocytes with an oral nucleoside analogue (AZT) for the protection of lymphocytes provides additive responses in all the parameters investigated.


Assuntos
Fármacos Anti-HIV/administração & dosagem , Eritrócitos , Macrófagos/virologia , Síndrome de Imunodeficiência Adquirida Murina/tratamento farmacológico , Animais , Fármacos Anti-HIV/uso terapêutico , Encéfalo/efeitos dos fármacos , Encéfalo/virologia , Relação CD4-CD8/efeitos dos fármacos , DNA Viral/análise , Didesoxinucleotídeos , Progressão da Doença , Portadores de Fármacos , Quimioterapia Combinada , Feminino , Glutationa/administração & dosagem , Glutationa/farmacologia , Glutationa/uso terapêutico , Rim/efeitos dos fármacos , Rim/imunologia , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/imunologia , Fígado/patologia , Linfócitos/efeitos dos fármacos , Linfócitos/imunologia , Linfócitos/virologia , Tecido Linfoide/efeitos dos fármacos , Tecido Linfoide/imunologia , Tecido Linfoide/patologia , Tecido Linfoide/virologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Mitógenos/farmacologia , Síndrome de Imunodeficiência Adquirida Murina/imunologia , Síndrome de Imunodeficiência Adquirida Murina/patologia , Síndrome de Imunodeficiência Adquirida Murina/virologia , Nucleotídeos de Timina/administração & dosagem , Nucleotídeos de Timina/farmacologia , Nucleotídeos de Timina/uso terapêutico , Zidovudina/administração & dosagem , Zidovudina/farmacologia , Zidovudina/uso terapêutico
15.
AIDS Res Hum Retroviruses ; 15(4): 345-53, 1999 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-10082118

RESUMO

Disseminated infection with Mycobacterium avium complex (MAC) remains the most common serious bacterial infection in patients with advanced AIDS. The organisms that make up this complex are found ubiquitously in the environment, yet rarely cause disseminated disease in nonimmunocompromised human patients; on the contrary, up to 50% of patients with AIDS may ultimately develop the pathology. Hence, therapeutic strategies able to inhibit HIV and Mycobacterium replication are needed. Because of the rapid plasma elimination and toxicity of the most commonly used drugs, daily multiple-drug therapies must often be continued throughout life, frequently causing major side effects and, as a consequence, poor patient compliance. Therefore, alternative strategies that reduce the toxicity of the drugs and allow prolonged application intervals are sorely needed. Since erythrocytes (RBCs) can behave as bioreactors able to convert impermeant prodrugs to membrane-releasable active drugs, new compounds (AZTpEMB, AZTpEMBpAZT, and AZTp2EMB) consisting of both an antiretroviral and an antimicrobial drug were designed and synthesized. Among these, only AZTp2EMB was hydrolyzed by erythrocyte enzymes and could be encapsulated inside RBCs. AZTp2EMB-loaded RBCs slowly released AZT and EMB in culture medium, reducing its concentration by one-half about every 48 hr of incubation at 37 degrees C. Moreover, when AZTp2EMB-loaded erythrocytes were incubated for 6 days in the presence of human macrophages infected with Mycobacterium avium (M. avium) a marked bactericidal effect (>1 log) was observed. Thus, AZTp2EMB-loaded erythrocytes could be used as endogenous bioreactors for AZT and EMB delivery in the treatment of HIV and M. avium infection.


Assuntos
Fármacos Anti-HIV/metabolismo , Antituberculosos/metabolismo , Eritrócitos/metabolismo , Etambutol/metabolismo , Zidovudina/metabolismo , Fármacos Anti-HIV/síntese química , Fármacos Anti-HIV/farmacologia , Antituberculosos/síntese química , Antituberculosos/farmacologia , Reatores Biológicos , Células Cultivadas , Dimerização , Etambutol/síntese química , Etambutol/farmacologia , Humanos , Estrutura Molecular , Mycobacterium avium/efeitos dos fármacos , Zidovudina/síntese química , Zidovudina/farmacologia
16.
FEBS Lett ; 426(1): 33-6, 1998 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-9598973

RESUMO

Inducible nitric oxide synthase (iNOS) is modulated at the transcriptional level. Overexpression of this protein may result in high levels of nitric oxide leading to tissue damage and immunosuppression. In order to reduce the pathological effects of NO overproduction many efforts have been devoted to the identification of specific inhibitors of iNOS. The discovery of peptide nucleic acids (PNA), a novel class of molecules able to selectively interact with nucleic acids, prompted us to attempt a new way for the regulation of NO production. Here we describe the synthesis, characterization and in vitro effects of a PNA molecule bearing a homopyrimidine sequence complementary to the 5' coding region of murine iNOS mRNA. This PNA shows specific interactions with iNOS mRNA in RNase protection assays and is able to block the synthesis of iNOS protein selectively in a rabbit reticulocyte lysate system. These results strengthen the view of a possible pharmacological application of PNA as a compound able to interfere with a specific enzymatic activity even at low concentrations.


Assuntos
Óxido Nítrico Sintase/genética , Oligonucleotídeos Antissenso/química , Animais , Sistema Livre de Células , Camundongos , Óxido Nítrico Sintase Tipo II , Hibridização de Ácido Nucleico , Peptídeos , Biossíntese de Proteínas/efeitos dos fármacos , RNA Mensageiro/química , Coelhos
17.
AIDS Res Hum Retroviruses ; 14(5): 435-44, 1998 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-9546803

RESUMO

Human herpesvirus (HSVs) are distributed worldwide and are among the most frequent causes of viral infection in HIV-1-immunocompromised patients. Hence, therapeutic strategies able to inhibit HSV-1 and HIV-1 replication are sorely needed. Until now, the most common therapies against HSV-1 and HIV-1 infectivity have been based on the administration of nucleoside analogs; however, to be active, these antiviral drugs must be converted to their triphosphorylated derivatives by viral and/or cellular kinases. At the cellular level, the main problems involved in the use of such drugs are their limited phosphorylation in some cells (e.g., antiretroviral drugs in macrophages) and the cytotoxic side effects of nucleoside analog triphosphates. To overcome these limitations, a new heterodinucleotide (AZTp2ACV) consisting of both an antiretroviral and an antiherpetic drug, bound by a pyrophosphate bridge, was designed and synthesized. The impermeant AZTp2ACV was encapsulated into autologous erythrocytes modified to increase their recognition and phagocytosis by human macrophages. Once inside macrophages, metabolic activation of the drug occurred. The addition of AZTp2ACV-loaded erythrocytes to human macrophages provided effective and almost complete in vitro protection from HIV-1 and HSV-1 replications, respectively. Therefore, AZTp2ACV acts as an efficient antiviral prodrug following selective targeting to macrophages by means of loaded erythrocytes.


Assuntos
Aciclovir/farmacologia , Antivirais/farmacologia , Eritrócitos , HIV-1/efeitos dos fármacos , Herpesvirus Humano 1/efeitos dos fármacos , Macrófagos/virologia , Replicação Viral/efeitos dos fármacos , Zidovudina/farmacologia , Aciclovir/administração & dosagem , Aciclovir/metabolismo , Animais , Antivirais/administração & dosagem , Antivirais/metabolismo , Chlorocebus aethiops , Cromatografia Líquida de Alta Pressão , Efeito Citopatogênico Viral , Combinação de Medicamentos , Eritrócitos/metabolismo , HIV-1/crescimento & desenvolvimento , Herpesvirus Humano 1/crescimento & desenvolvimento , Humanos , Macrófagos/fisiologia , Fagocitose , Células Vero , Zidovudina/administração & dosagem , Zidovudina/metabolismo
18.
J Acquir Immune Defic Syndr Hum Retrovirol ; 17(3): 189-95, 1998 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-9495216

RESUMO

A new antiretroviral drug (azidothymidine homodinucleotide, AZTp2AZT), designed for the protection of macrophages against retroviral infection, was evaluated in a murine retrovirus-induced immunodeficiency model of AIDS (MAIDS) alone and in combination with oral azidothymidine (AZT). C57BL/6 mice were infected with the retroviral complex LP-BM5 and treated for 3 months by weekly administrations of 15 nmol of AZTp2AZT encapsulated into autologous erythrocytes for macrophage protection. AZTp2AZT treatment was found to reduce lymphoadenopathy (48%), splenomegaly (26%), and BM5d proviral DNA content in lymph nodes, spleen, and brain of 37%, 40%, and 36%, respectively, compared with untreated animals. AZT administration in drinking water (0.25 mg/ml) was more effective than administration of AZTp2AZT encapsulated into erythrocytes in reducing lymphoadenopathy, splenomegaly, gammaglobulinemia, and proviral DNA content in lymph nodes, but it caused a reduction in erythrocyte count and hematocrit levels. Although combined treatments do not provide additive responses in the several parameters investigated, they were found to be much more effective in reducing the proviral DNA content in brain (67%) than were monotherapies. Furthermore, no apparent signs of hematotoxicity were observed. Thus, macrophage delivery of antiviral drugs may contribute to brain protection from retroviral infections by mechanisms other than those exerted by oral AZT administration.


Assuntos
Fármacos Anti-HIV/uso terapêutico , Síndrome de Imunodeficiência Adquirida Murina/tratamento farmacológico , Pró-Fármacos/uso terapêutico , Nucleotídeos de Timina/uso terapêutico , Zidovudina/uso terapêutico , Animais , Encéfalo/virologia , DNA Viral/análise , Didesoxinucleotídeos , Quimioterapia Combinada , Feminino , Citometria de Fluxo , Imunoglobulina G/sangue , Injeções Intraperitoneais , Linfonodos/virologia , Doenças Linfáticas/tratamento farmacológico , Ativação Linfocitária/efeitos dos fármacos , Contagem de Linfócitos/efeitos dos fármacos , Macrófagos/virologia , Camundongos , Camundongos Endogâmicos C57BL , Síndrome de Imunodeficiência Adquirida Murina/imunologia , Síndrome de Imunodeficiência Adquirida Murina/virologia , Pró-Fármacos/administração & dosagem , Pró-Fármacos/farmacologia , Baço/virologia , Esplenomegalia/tratamento farmacológico , Nucleotídeos de Timina/administração & dosagem , Nucleotídeos de Timina/farmacologia
19.
Biochimie ; 80(11): 923-31, 1998 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-9893952

RESUMO

L-fucose and L-rhamnose are two 6-deoxyhexoses naturally occurring in several complex carbohydrates. In prokaryotes both of them are found in polysaccharides of the cell wall, while in animals only L-fucose has been described, which mainly participates to the structure of glycoconjugates, either in the cell membrane or secreted in biological fluids, such as ABH blood groups and Lewis system antigens. L-fucose and L-rhamnose are synthesized by two de novo biosynthetic pathways starting from GDP-D-mannose and dTDP-D-glucose, respectively, which share several common features. The first step for both pathways is a dehydration reaction catalyzed by specific nucleotide-sugar dehydratases. This leads to the formation of unstable 4-keto-6-deoxy intermediates, which undergo a subsequent epimerization reaction responsible for the change from D- to L-conformation, and then a NADPH-dependent reduction of the 4-keto group, with the consequent formation of either GDP-L-fucose or dTDP-L-rhamnose. These compounds are then the substrates of specific glycosyltransferases which are responsible for insertion of either L-fucose or L-rhamnose in the corresponding glycoconjugates. The enzyme involved in the first step of GDP-L-fucose biosynthesis in E. coli, i.e., GDP-D-mannose 4,6 dehydratase, has been recently expressed as recombinant protein and characterized in our laboratory. We have also cloned and fully characterized a human protein, formerly named FX, and an E. coli protein, WcaG, which display both the epimerase and the reductase activities, thus indicating that only two enzymes are required for GDP-L-fucose production. Fucosylated complex glycoconjugates at the cell surface can then be recognized by specific counter-receptors in interacting cells, these mechanisms initiating important processes including inflammation and metastasis. The second pathway starting from dTDP-D-glucose leads to the synthesis of antibiotic glycosides or, alternatively, to the production of dTDP-L-rhamnose. While several sets of data are available on the first enzyme of the pathway, i.e., dTDP-D-glucose dehydratase, the enzymes involved in the following steps still need to be identified and characterized.


Assuntos
Fucose/metabolismo , Ramnose/metabolismo , Animais , Carboidratos Epimerases/metabolismo , Fucose/biossíntese , Guanosina Difosfato Fucose/metabolismo , Humanos , Hidroliases , Modelos Químicos , Ramnose/biossíntese
20.
Biol Bull ; 195(2): 120-125, 1998 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28570172

RESUMO

The skeleton of the common Mediterranean demosponge Chondrosia reniformis lacks endogenous spicules; but exogenous siliceous material is selectively incorporated into its collagenous ectosome, strengthening this layer. Nevertheless, the settling of sponge buds during asexual reproduction necessitates an active incorporation of the calcareous substratum through the sponge lower ectosome. This fact suggests the presence of a polarity in the sponge, with the lower surface selecting primarily carbonates, and the upper surface selecting exclusively silicates and quartz. Our observations under experimental conditions showed that the strong selectivity of the upper ectosome is realized only when the sponge is fixed to the substratum; if detached, the sponge incorporates both quartz and carbonates. In laboratory experiments, the incapacity of both kinds of ectosome to regenerate into a new complete sponge suggests that this polarity arises early in ontogeny.

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