Polymer coatings based on sulfonated-poly-ether-ether-ketone films for implant dentistry applications.

Poly-ether-ether-ketone (PEEK) is one of the most important biocompatible polymers and its sulfonation has been studied for biomedical applications. The aim of the present study is to produce, to characterize and to assess bioactivity of PEEK coatings with sulfonated PEEK (SPEEK) films. Biomedical grade PEEK (Invibio®, Batch: D0602, grade: NI1) was functionalized using sulfuric acid 98%. SPEEK was dissolved into DMSO or into DMF, both at 10% mass/volume. PEEK bars (N = 18) and cylinders (N = 27) were manufactured by compression molding and heating. SPEEK/DMSO and SPEEK/DMF were drop casted at PEEK bars and dip coated at PEEK cylinders (PEEK + SPEEK/DMSO and PEEK + SPEEK/DMF). Characterization was performed through Fourier Transform Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Scanning Electron Microscope (SEM) and contact angle measurements. Bioactivity was assessed by immersion of samples at SBF for 1, 7 and 21 days, followed by SEM, energy-dispersive analysis (EDX) and FTIR analysis. Statistical analysis was carried out by one-way analysis of variance (ANOVA) (p = 0.05). Characteristic bands of PEEK and SPEEK, were identified through FTIR spectrum analysis, while semicrystallinity was confirmed by XRD. PEEK + SPEEK/DMF showed more evident physicochemical modifications. PEEK + SPEEK/DMSO provided a more regular and hydrophobic surface, observed through SEM and contact angle measurements. SEM/EDX showed that precipitates of calcium were formed at PEEK + SPEEK/DMSO and PEEK + SPEEK/DMF at all experimental times, but materials were not considered bioactive. Interesting surface properties were achieved with SPEEK coatings but the production of SPEEK films at PEEK surface has to be further improved and biologically tested. Schematic diagram showing the methodology applied in this study to prepare PEEK and SPEEK samples, as well as the promising application of the material.

Synergistic interactions between corrosion and wear at titanium-based dental implant connections: A scoping review.

Two-piece implant systems are mainly used in oral implantology involving an osseointegrated implant connected to an abutment, which supports prosthetic structures. It is well documented that the presence of microgaps, biofilms and oral fluids at the implant-abutment connection can cause mechanical and biological complications. The aim of this review paper was to report the degradation at the implant-abutment connection by wear and corrosion processes taking place in the oral cavity. Most of the retrieved studies evaluated the wear and corrosion (tribocorrosion) of titanium-based materials used for implants and abutments in artificial saliva. Electrochemical and wear tests together with microscopic techniques were applied to validate the tribocorrosion behavior of the surfaces. A few studies inspected the wear on the inner surfaces of the implant connection as a result of fatigue or removal of abutments. The studies reported increased microgaps after fatigue tests. In addition, data suggest that micromovements occurring at the contacting surfaces can increase the wear of the inner surfaces of the connection. Biofilms and/or glycoproteins act as lubricants, although they can also amplify the corrosion of the surfaces. Consequently, loosening of the implant-abutment connection can take place during mastication. In addition, wear and corrosion debris such as ions and micro- and nanoparticles released into the surrounding tissues can stimulate peri-implant inflammation that can lead to pathologic bone resorption.

Inhibition of multi-species oral biofilm by bromide doped bioactive glass.

Bioactive glass is an attractive biomaterial that has shown excellent osteogenic and angiogenic effects for oral bone repairing procedures. However, anti-biofilm potential related to such biomaterial has not been completely validated, mainly against multi-species biofilms involved in early tissue infections. The aim of the present study was to evaluate the anti-biofilm effect of 58 S bioactive glass embedding calcium bromide compounds at different concentrations. Bioactive glass free or containing 5, or 10 wt % CaBr2 was synthesized by alkali sol-gel method and then characterized by physco-chemical analyses and scanning electron microscopy (SEM). Then, samples were tested by microbiological assays using optical density, real time q-PCR, and SEM. Bioactive glass particles showed accurate chemical composition and an angular shape with a bimodal size distribution ranging from 0.6 to 110 µm. The mean particle size was around 29 µm. Anti-biofilm effect was recorded for 5 wt % CaBr2 -doped bioactive glass against S. mitis, V. parvula, P. gingivais, S. gordoni, A. viscosus, F, nucleatum, P. gingivais. F. nucleatum, and P. gingivalis. Such species are involved in the biofilm structure related to infections on hard and soft tissues in the oral cavity. The incorporation of calcium bromide into bioactive glass can be a strategy to enhance the anti-biofilm potential of bioactive glasses for bone healing and infection treatment. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1994-2003, 2017.

In vivo electrical application on titanium implants stimulating bone formation.

BACKGROUND AND OBJECTIVE: The aim of the present in vivo study was to measure the bone implant contact area after electrical stimulation of dental implants. MATERIAL AND METHODS: Ninety titanium dental implants (6 mm × 11.5 mm) with a smooth surface were placed in six male Beagle dogs and then the implant-bone interfaces was assessed by histological analyses after 7 and 15 d. The 12-mo-old dogs, with a weight of 15 kg, were randomly divided into two groups based on the duration of bone healing: 7 and 15 d. Also, implants were divided into three groups based on electrical stimulation: group A, 10 µA; group B, 20 µA; and group C, control group. The electrical current was applied by an electrical device coupled to the implant connection. RESULTS: After 7 d of electrical stimulation, no statistical differences in bone-implant interface contact area were observed. However, a significantly higher bone-implant interface contact area was recorded for group B than for groups A and C (p < 0.01) after 15 d. No statistical difference was observed between groups A and C (p > 0.05). CONCLUSION: The electrical stimulation of dental implants can generate a larger area of bone-implant interface contact as a result of bone formation. Factors such as different electrical current intensity and duration should be studied in further work to clarify the potential of this method.

Lactam inhibiting Streptococcus mutans growth on titanium.

The aim of this work was to analyze the activity of novel synthetic lactams on preventing biofilm formation on titanium surfaces. Titanium (Ti6Al4V) samples were exposed to Streptococcus mutans cultures in the presence or absence of a synthetic lactam. After 48h incubation, planktonic growth was determined by spectrophotometry. Biofilm was evaluated by crystal violet staining and colony forming units (CFU·ml(-)(1)), followed by scanning electron microscopy (SEM). Results showed that the average of adhered viable cells was approximately 1.5×10(2)CFU/ml in the presence of lactam and 4×10(2)CFU/ml in its absence. This novel compound was considerable active in reducing biofilm formation over titanium surfaces, indicating its potential for the development of antimicrobial drugs targeting the inhibition of the initial stages of bacterial biofilms on dental implants abutments.