Hot and cold whirlpool treatments and knee joint laxity.

OBJECTIVE: To examine the influence of clinical applications of heat and cold on arthrometric laxity measurements of the knee. DESIGN AND SETTING: The knee joint was submersed 4 inches above the patella in hot and cold whirlpools containing water of 40 degrees C and 15 degrees C for 20 minutes. A control was also performed to provide a neutral temperature comparison group. SUBJECTS: Eight males and 7 females with no history of knee injury. MEASUREMENTS: The knee was maintained at 20 degrees flexion and tibial rotation at either 15 degrees of internal rotation, 15 degrees of external rotation, or a neutral measurement with a modified KT-1000 knee arthrometer equipped with an LCCB-50 strain gauge that allowed for the digital display of the applied distraction forces. Order of testing was counterbalanced. Subjects underwent each condition once, with each trial on separate days. Two 2-factor repeated measure analyses of variance were performed to test effects of temperature on knee laxity for the dependent measure (laxity at 89N and at maximal displacement forces). RESULTS: There was no thermal effect on displacement at 89N nor at maximal distraction (p > .05). A difference was found with respect to test position, with external rotation showing a greater displacement than internal rotation (p < .05). CONCLUSIONS: There was no evidence that hot or cold whirlpool treatments alter knee laxity as assessed with the KT-1000. Rotation of the tibia does affect the magnitude of displacement of the knee. Further research is needed to determine if these findings can be applied to ACL-deficient or ACL-reconstructed knees.

Colonization control of lactose-fermenting Salmonella typhimurium in young broiler chickens by use of dietary lactose.

Inclusion of lactose in the diets of chickens has been determined to reduce cecal colonization with Salmonella typhimurium. We hypothesized, therefore, that dietary lactose may be a practical means for reducing the prevalence of Salmonella contamination of chicken products. Because some strains of Salmonella are atypical and ferment lactose, we investigated the effects of dietary lactose on cecal colonization with lactose-fermenting S typhimurium. Broiler chicks were inoculated intracloacally with Lac+ S typhimurium selected for resistance to novobiocin and rifampicin. The chicks also were inoculated orally with certain anaerobes that do not effectively inhibit colonization by S typhimurium, but do appear essential for lactose mediated inhibition of cecal colonization. Control chicks were not given dietary lactose, and chicks in the experimental group were fed a diet containing 7% lactose. Enumeration of Lac+ S typhimurium in cecal contents revealed dietary lactose to be effective at controlling this organism. Control was correlated with changes in cecal pH and increases in undissociated volatile fatty acids, especially propionic acid.

Activation and germination of Bacillus thuringiensis spores in Manduca sexta larval gut fluid.

Incubation of Bacillus thuringiensis HD-1 spores in the larval gut fluid of Manduca sexta (tobacco hornworm) resulted in increased viable counts, conversion to phase-dark spores, and a loss of absorbance in spore suspensions, indicative of spore germination. Heat-activated and untreated spores incubated in water did not exhibit these changes. Only when spores were heat activated and incubated in germinants L-alanine and adenosine did changes in the spores approximate those observed in gut fluid. These data suggest that M. sexta larval gut fluid induces the activation and germination of B. thuringiensis spores.

Plasmid-associated sensitivity of Bacillus thuringiensis to UV light.

Spores and vegetative cells of Bacillus thuringiensis were more sensitive to UV light than were spores or cells of plasmid-cured B. thuringiensis strains or of the closely related Bacillus cereus. Introduction of B. thuringiensis plasmids into B. cereus by cell mating increased the UV sensitivity of the cells and spores. Protoxins encoded by one or more B. thuringiensis plasmids were not involved in spore sensitivity, since a B. thuringiensis strain conditional for protoxin accumulation was equally sensitive at the permissive and nonpermissive temperatures. In addition, introduction of either a cloned protoxin gene, the cloning vector, or another plasmid not containing a protoxin gene into a plasmid-cured strain of B. thuringiensis all increased the UV sensitivity of the spores. Although the variety of small, acid-soluble proteins was the same in the spores of all strains examined, the quantity of dipicolinic acid was about twice as high in the plasmid-containing strains, and this may account for the differences in UV sensitivity of the spores. The cells of some strains harboring only B. thuringiensis plasmids were much more sensitive than cells of any of the other strains, and the differences were much greater than observed with spores.