[Aggregation of denatured membrane proteins--the initial stage of acid hemolysis]. / Agregatsiia denaturirovannykh membrannykh belkov--nachal'nyi étap kislotnogo gemoliza.

Influence of some sulfhydryl reagents on kinetics of acid hemolysis has been investigated. The results obtained indicate that the mechanism of acid hemolysis includes aggregation of some denatured membrane integral proteins, following the intermolecular disulfide bond formation. A suggestion is made that in this hemolysis-inducing aggregation free SH-groups containing proteins only take place.

[Initiation of peroxidation of liposome membrane lipids by activated polymorphonuclear leukocytes]. / Initsirovanie perekisnogo okisleniia lipidov membran liposom aktivirovannymi polimorfnoiadernymi leikotsitami krovi.

The influence of active oxygen forms produced by zymosan-stimulated polymorphonuclear leukocytes (PMNL) on the initiation of liposome lipid peroxidation has been studied. It has been shown, by measuring the concentration of TBA-active products, that lipid peroxidation induced by PMNL stimulation occurs only in the presence of Fe-ADP. This fact demonstrates that OH'-radicals are responsible for the initiation of lipid peroxidation. Superoxide dismutase and catalase almost completely inhibited PMNL-stimulated peroxidation. The results obtained suggest that active oxygen forms that appear during PMNL stimulation can migrate at a considerable distance from the place of their origin, initiating peroxidation of cell membrane lipids and lipoproteins in the presence of Fe ions, which seems to underlie bacteriocidal and cytotoxic action of phagocytes.