Morphometric analysis of bone vascular channels during the biointegration of autologous bone grafts.

Vascularization of the transplanted bone tissue is a key factor for success and precedes the formation of bone tissue. Vascularized bone grafts have been widely used in bone transplantation for their efficiency. Maturation of the bone tissue at the place of the transplant involves the change in the vascular patterning, from plexiform irregular vascular networks, to regular, polygonal networks following the structure of osteons. Seven dogs were performed bone transplantation and intravenous injection with China ink in order to highlight the network of blood vessels during bone formation at the place of the transplant. The area occupied by blood vessels increased during osteogenesis, and their length decreased in evolution, suggesting a maturation of the vascular pattern.

Microdensity and morphometric analysis of autologous bone grafts cells.

Bone transplantation as a mean to reduce the fracture healing time in large defects was attempted for the first time more than 300 years ago, with nowadays several techniques and methods of assessment of its efficacy. The bone graft was longtime thought as initiating the osteogenesis from the recipient, but new data show that cells from the graft contribute to osteogenesis and to its incorporation into newly formed bone. There is no accurate assessment of the microdensity of bone graft cells in evolution so far, the only studies published recently referring to newly formed bone area. We have performed bone transplantation on seven dogs and have analyzed the microdensity and the morphometric features of bone graft cells. We have found that the cellular microdensity increases both in the graft and in the recipient bone, simultaneously with a decrease of cell size and circularity during maturation.

[Incidence and sensitivity to antibiotics of germs isolated from surgical wound infections]. / Studiu privind incidenta si sensibilitatea la antibiotice a germenilor izolati din infectii postoperatorii de plaga chirurgicala.

We studied 119 bacterial strains isolated from postoperative infected wounds. All these strains were considered to be strains that derived from the same number of nosocomial infections, all the postoperative infections of the wounds being nosocomial infections. Regarding their frequency, we isolated the following strains: E. coli--68 strains (57%), S. aureus--37 strains (31%), Pseudomonas spp.--9 strains (8%) and Proteus spp.--5 strains (4%). We performed the bacteriological study of these strains and some correlations between them and the surgical diagnosis. In order to help the surgical therapy of the overinfected wounds, we tested the drug sensitivity of all these strains. The results regarding the drug sensitivity show that these strains have different types of resistance to antibiotics, the Pseudomonas strains being the most resistant. We noticed a continuous decrease of the sensitivity of the isolated strains to certain antimicrobial drugs, and this focuses the attention on the necessity of monitoring the antibiotic prescriptions in hospitals. Taking into account the results obtained after this study, we should reconsider the concept of nosocomial infections control through prevention activities, in order to reduce the incidence and to identify the potential causes that can lead to nosocomial infections.