RESUMO
An 80-year-old white man presented with gangrenous lesions involving several distal phalanges of his left hand and an elevated erythrocyte sedimentation rate (ESR). Temporal artery biopsy showed patchy destruction of the internal elastic lamina by mononuclear cell infiltration, consistent with the diagnosis of temporal arteritis. After amputation of gangrenous lesions, he was discharged taking prednisone (60 mg/day). Twelve months after discharge there was no recurrence of ischemic manifestations and ESR was normal. Association of digital gangrene and elevated ESR should alert the clinician toward this diagnosis once other diseases such as atherosclerosis, scleroderma, lupus erythematosus, periarteritis nodosa have been ruled out.
Assuntos
Dedos/patologia , Arterite de Células Gigantes/patologia , Idoso , Idoso de 80 Anos ou mais , Dedos/cirurgia , Gangrena , Arterite de Células Gigantes/complicações , Humanos , MasculinoRESUMO
Three unlinked genes ARGRI, ARGRII and ARGIII are necessary to control the synthesis of the arginine anabolic and catabolic genes. The three genes have been cloned and sequenced and we report here the results for the ARGRI and ARGRIII genes. They encode proteins of 177 and 355 amino acids, respectively. The ARGRIII protein has a very acidic carboxy-terminus (17 aspartate residues). From a comparison of the sequences, the ARGRI and ARGRIII gene products do not show the common characteristics of other DNA binding proteins (nuclear localization and putative DNA binding site) in contrast to the ARGRII regulatory protein. The transcription of both genes is not affected by the presence of arginine in the growth medium.
Assuntos
Arginina/metabolismo , Proteínas Fúngicas/genética , Regulação da Expressão Gênica , Genes Fúngicos , Saccharomyces cerevisiae/genética , Sequência de Aminoácidos , Sequência de Bases , DNA Fúngico/genética , Proteínas Fúngicas/fisiologia , Teste de Complementação Genética , Peso Molecular , Saccharomyces cerevisiae/metabolismoRESUMO
Three regulatory proteins are involved in the control of arginine metabolism in yeast: ARGRI, ARGRII and ARGRIII. The control region and part of the coding sequence of the ARGR genes were fused to the Escherichia coli lacZ gene. These chimeras were used to study the expression of the regulatory genes as well as the cellular compartmentalization of the regulatory products. Our results show that the three ARGR proteins are localized in the nucleus and that their synthesis is not regulated by arginine nor by any of the other ARGR products. However, some data suggest that the ARGRIII protein could control ARGRI activity.
Assuntos
Arginina/metabolismo , Genes Fúngicos , Genes Reguladores , Saccharomyces cerevisiae/genética , Núcleo Celular/análise , Proteínas Fúngicas/genética , Proteínas Fúngicas/fisiologia , Regulação da Expressão Gênica , Proteínas Recombinantes de Fusão/metabolismo , Saccharomyces cerevisiae/metabolismoRESUMO
Based on nucleotide sequence determination, we have identified two new yeast genes FUN80 and FUN81 located on chromosome XIII. They are both essential for cellular growth but their function is still unknown. FUN80 is closely linked to the ARGRI (or ARG80) gene while FUN81 is located next to the ARGRII (or ARG81) gene. Interestingly, the proteins encoded by these two genes have a long stretch of acidic amino acids within their C-terminal portions.
