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Syst Appl Microbiol ; 26(3): 453-65, 2003 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-14529189

RESUMO

We have analysed 198 fast-growing soybean-nodulating rhizobial strains from four different regions of China for the following characteristics: generation time; number of plasmids; lipopolysaccharide (LPS), nodulation factors (LCOs) and PCR profiles; acidification of growth medium; capacity to grow at acid, neutral, and alkaline pH; growth on LC medium; growth at 28 and 37 degrees C; melanin production capacity; Congo red absorption and symbiotic characteristics. These unbiased analyses of a total subset of strains isolated from specific soybean-cropping areas (an approach which could be called "strainomics") can be used to answer various biological questions. We illustrate this by a comparison of the molecular characteristics of five strains with interesting symbiotic properties. From this comparison we conclude, for instance, that differences in the efficiency of nitrogen fixation or competitiveness for nodulation of these strains are not apparently related to differences in Nod factor structure.


Assuntos
Glycine max/microbiologia , Rhizobium/fisiologia , Simbiose , Proteínas de Bactérias/análise , China , Vermelho Congo/metabolismo , Impressões Digitais de DNA , DNA Bacteriano/isolamento & purificação , DNA Ribossômico/análise , DNA Espaçador Ribossômico/análise , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Lipopolissacarídeos/análise , Melaninas/biossíntese , Plasmídeos , Polimorfismo de Fragmento de Restrição , RNA Ribossômico 16S/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico , Rhizobium/química , Rhizobium/genética , Rhizobium/isolamento & purificação
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