RESUMO
Anti-galactoside rabbit antisera increased the frequency of slow (atypical) MEPPs. No lysis of the Schwann cells was found in synaptic regions. The data obtained suggest that fixation of antibodies to galactocerebroside on the Schwann cell surface membrane alters the cell's activity thus affecting some characteristics of neuromuscular transmission.
Assuntos
Junção Neuromuscular/fisiologia , Células de Schwann/fisiologia , Transmissão Sináptica/fisiologia , Animais , Antígenos de Superfície/imunologia , Galactosilceramidas/imunologia , Soros Imunes/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Microeletrodos , Junção Neuromuscular/efeitos dos fármacos , Perfusão/métodos , Coelhos , Ranidae , Células de Schwann/efeitos dos fármacos , Células de Schwann/imunologia , Transmissão Sináptica/efeitos dos fármacosRESUMO
The end-plate currents and potentials (EPCs and EPPs) in cut or curarized muscles were investigated in frogs maintained at different temperatures (5 and 20-25 degrees C) before experiments in summer and in winter. EPCs and EPPs were elicited by nerve stimulation with different frequencies (1 and 100/s). Seasonal changes in amplitude, facilitation and depression of the EPPs and EPCs observed in curarized and cut (not curarized) muscles differed quantitatively and qualitatively. These differences may be explained assuming that sensitivity of the neuromuscular junction to the curare action (probably, a presynaptic one) is season-variable.
Assuntos
Junção Neuromuscular/fisiologia , Estações do Ano , Transmissão Sináptica/fisiologia , Animais , Potenciais Evocados/efeitos dos fármacos , Potenciais Evocados/fisiologia , Placa Motora/efeitos dos fármacos , Placa Motora/fisiologia , Junção Neuromuscular/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacos , Temperatura , Tubocurarina/farmacologiaRESUMO
The end-plate potentials (EPPs) were investigated in frog neuromuscular junctions with different nerve endings structure (cutaneous pectoris muscle). When the muscle fibres input resistance and nerve endings length in selected pairs of synapses were similar, larger EPPs amplitudes were noted in synapses with myelin segments within nerve ending. It is suggested, that myelinization within nerve ending is one of the factors of synaptic efficacy.