RESUMO
A dependence was established between the cytogenetic effect of dioxydinum antibacterial drug and the disturbance of carbohydrate metabolism in rats with alloxan diabetes.
Assuntos
Aberrações Cromossômicas , Diabetes Mellitus Experimental/metabolismo , Mutagênicos/farmacologia , Quinoxalinas/farmacologia , Animais , Medula Óssea/efeitos dos fármacos , Metabolismo dos Carboidratos , Relação Dose-Resposta a Droga , Interações Medicamentosas , Insulina/farmacologia , Masculino , Metáfase/efeitos dos fármacos , RatosRESUMO
Cytochemical methods were used to explore the changes in the activity of redox (succinate dehydrogenase, mitochondrial alpha-glycerophosphate dehydrogenase) and hydrolytic (acid, alkaline phosphatase, myeloperoxidase) enzymes of peripheral blood leukocytes of random-bred albino rats during simulation of toxic hepato-, nephropathy and hemic hypoxia of different gravity. The studies of the changes of leukocyte distribution according to the activity of the above enzymes were demonstrated to be informative for the diagnosis of prepathological conditions. The possibility was also shown of differentiating the gravity of the pathological conditions with the aid of an analysis of the enzymes under study by the method of linear discriminant functions.
Assuntos
Ensaios Enzimáticos Clínicos , Leucócitos/enzimologia , Animais , Intoxicação por Tetracloreto de Carbono/diagnóstico , Doença Hepática Induzida por Substâncias e Drogas/diagnóstico , Histocitoquímica , Hipóxia/induzido quimicamente , Hipóxia/diagnóstico , Nefropatias/induzido quimicamente , Nefropatias/diagnóstico , Leucócitos/efeitos dos fármacos , Masculino , Intoxicação por Mercúrio/diagnóstico , Ratos , Nitrito de Sódio/intoxicaçãoRESUMO
A cytogenetic study of bone marrow cells was carried out first under conditions of toxic hepatopathy, experimental toxic nephropathy, alloxan diabetes in rats and under conditions of colibacillar and pyocyanic sepsis in mice in different periods of pathology. These experimental models may be used in research on medicinal mutagenesis.
Assuntos
Aberrações Cromossômicas , Modelos Animais de Doenças , Animais , Medula Óssea/ultraestrutura , Doença Hepática Induzida por Substâncias e Drogas/genética , Diabetes Mellitus Experimental/genética , Infecções por Escherichia coli/genética , Feminino , Nefropatias/induzido quimicamente , Nefropatias/genética , Masculino , Metáfase , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Infecções por Pseudomonas/genética , RatosRESUMO
Certain new nonionogenic detergents which are hydroxy-polyethoxy-derivatives of dodecane show in vitro bacteriostatic action as related to gram-positive microorganisms. In this respect they are not inferior to chloride cetylpyridinium or sodium dodecyl sulphate. They are similar to chloride cetylpyridinium in their ability to disrupt the osmotic barrier of protoplasts and to inhibit NAD-H-oxidase of membrane fractions of Micrococcus lysodeikticus. Unlike chloride cetylpyridinium and sodium dodecyl sulphate, nonionogenic detergents do not inhibit membrane-bound ATPase of these bacteria.
Assuntos
Anti-Infecciosos Locais , Bactérias/efeitos dos fármacos , Detergentes/farmacologia , Adenosina Trifosfatases/antagonistas & inibidores , Animais , Membrana Celular/efeitos dos fármacos , Cetilpiridínio/farmacologia , Depressão Química , Detergentes/toxicidade , Camundongos , NADH NADPH Oxirredutases/antagonistas & inibidores , Consumo de Oxigênio/efeitos dos fármacos , Protoplastos/efeitos dos fármacos , Dodecilsulfato de Sódio/farmacologiaRESUMO
A procedure for determining orientation reaction after Boissié et al (1964) was modified for albino rats. In determining the thresholds of acute action of phthalates and carboran in comparison with summation-threshold index, registration of spontaneous motor activity and orientation reaction according to the number of risings in a jar the proposed method is shown to be 5-10 times more sensitive.
