[Legal and forensic medical problems of postmortem donation]. / Pravovye i sudebno-meditsinskie problemy posmertnogo donorstva.

The article is devoted to legal and forensic medical problems of postmortem donation. The substantive provisions of postmortem donation, as well as normative legal documents regulating the processes of organs harvesting from deceased persons for subsequent transplantation and governing the work of transplantologists and forensic medical experts have been considered. The practical examples illustrating the essence and nature of the problem of postmortem forensic medical expertise of persons with absent organs has been given and the importance of the participation of a forensic medical expert involved in the decision-making process on possibility (or impossibility) of the corpse's organs and tissues explantation without prejudice to the further expert examination has been emphasized. The authors pay particular attention to the inadequacy of the legal framework, including the lack of a clear understanding of the legal status of the person holding the position of forensic medical expert, who provides an expert opinion on the organs' explantation.

[Possibilities of educational process and researches realization using organs and tissues of the deceased person]. / Vozmozhnosti realizatsii obrazovatel'nogo protsessa i nauchnykh issledovanii s ispol'zovaniem organov i tkanei umershego.

The article presents the results of analysis of doctors' practical training as well as scientific research using organs and tissues of the deceased person. The main problematic aspects preventing appropriate realization of the possibility to use the unclaimed bodies, organs and tissues of the deceased person for these purposes are highlighted based on the study of regulatory framework controlling these activities, scientific publications on defined topic, as well as the direct authors' participation in the training of resident doctors and PhD students.

[Indirect molecular genetic predisposition factors to increased thrombosis in sufferers with mechanical lower limb trauma]. / Nepryamye molekulyarno-geneticheskie faktory predraspolozhennosti k povyshennomu tromboobrazovaniyu u poterpevshikh s mekhanicheskoi travmoi nizhnikh konechnostei.

THE AIM OF THE STUDY: Was to selectively determine the occurrence frequency of polymorphic alleles in candidate genes of hereditary predisposition to increased thrombosis (T) in persons with mechanical trauma of musculoskeletal system (MS), who died from pulmonary artery thromboembolia (PATE). A total of 48 deaths from PATE cases of sufferers with MS trauma. The character of single nucleotide polymorphisms carriage (SNPC) in 13 candidate genes of hereditary predisposition to increased T (in genes, responsible for the synthesis of plasma proteins of the hemostatic system, platelet factors affecting tension of vessel wall and folate cycle) was determined. It has been established that the most common «mutant¼ alleles are found in PAI-1 -675 5G/4G, MTHFR 677 CT and MTRR 66AG genes, and in 87.8, 53.85 and 75.0% of the analysed cases, respectively. The consideration of SNP carriage character in the genes of predisposition to increased T is required for the full expert judgement on the causality between a mechanical trauma and PATE.

[Microcirculation in fractures of long bones of the lower extremity]. / Kharakteristika mikrotsirkulyatornogo rusla pri perelomakh dlinnykh trubchatykh kostei nizhnei konechnosti.

The study objective was to investigate soft tissue microcirculation in the area of a lower limb long bone fracture. We studied fractures of the lower limb long bones from 0 to 7 days of age in 17 males and 37 females. The mean age of patients was 57.33±2.53 years. Histological examination of soft tissues from the area of the lower limb long bone fracture and at the distance from the fracture was performed. Standard hematoxylin and eosin staining was used. The thickness and diameter of arterioles, venules, and capillaries were evaluated by morphometry of the sections made. The observed pattern reflects the response of the microcirculation of the soft tissues to the mechanical impact. The relative ratio of the artery diameter to the small-caliber vein diameter was 1.01±0.13, and the artery wall thickness to the vein wall thickness was 2.03±0.28, which can be considered a natural reaction of the microcirculation blood vessels to the mechanical impact in 1-1.5 weeks after the formed fracture of the lower limb long bone.

[Morphofunctional characteristics of the heart in sudden death of children under 1 year of age in the forensic medical aspect]. / Morfofunktsional'naya kharakteristika serdtsa pri vnezapnoi smerti detei do 1 goda v sudebno-meditsinskom aspekte.

The aim of this study is to describe the morphological and functional characteristics of the cardiac in sudden infants death syndrome (SIDS) in children under one year of age. Twenty eight cases of SIDS were studied histologically and immunohistochemically. Histological examination of the sectional material was carried out using standard and additional stains. The study of cardiac muscle tissue with routine staining with haematoxylin-eosin revealed interstitial oedema, uneven plethora of blood vessels: dystonia and weak plethora of part of the coronary arteries, excess of red blood cells in the veins as well as microcirculatory plethora with stasis of the erythrocytes. An immunohistochemical study revealed a mild over expression of p53 in cellular elements, small-focal expression of CD68 in cardiomyocytes apoptosis intramural areas, activation of mast cells (CD117), expression of ki-67 in macrophages, proliferation of fibroblasts. Additional forensic criteria for the diagnosis of SIDS were determined in the form of atrial endocardial fibrosis and interventricular septum; the expression of CD68, CD117 in fibroblasts, mast cells and lymphocytes; apoptosis of cardiomyocytes (expression of p53), proliferation of fibroblasts and remodeling of the heart (expression of ki-67).

[Methodological basis for determining a person's age]. / Metodologicheskie osnovy opredeleniya vozrasta cheloveka.

This paper presents a review of modern fundamental research studies investigating the age-related changes of connective, cartilage, nervous and bone tissues. A methodological and mathematical basis for the analysis of the obtained results is proposed. The conducted analysis enabled the formulation of a new scientific direction - the forensic medical determination of age in the identification of individuals in emergency situations with mass casualties. A scientifically grounded theory of digital forensic diagnostics of age, general regularities of development and some differences of age adaptation of connective, cartilage, nervous and bone tissues, including the phenomena of heterochronicity, heterotopy and heterogeneity resulted. The results of this research determine the direction of further research by improving the methods of quantitative age morphology, molecular autopsy, digital technologies and the formation of a technologically based concept for the registration of identifying biological features.

[Molecular genetic basis of sudden cardiac death in the young with cardiomyopathy of various origins]. / Molekuliarno-geneticheskie osnovy vnezapnoi serdechnoi smerti lits molodogo vozrasta s kardiomiopatiei razlichnogo geneza.

This paper provides a review of the modern literature devoted to the problem of forensic medical interpretation of the molecular genetic research of the young who died suddenly. The authors attempted to draw a parallel between the morphological markers of different variants of cardiomyopathy as the most common disease in sudden death at a young age and the association with genetic mutations in the genes responsible for the synthesis of sarcomer proteins, desmos and membrane channels. Based on the results of the analysis, further research is proposed to improve the accuracy of forensic diagnosis in cases of young deaths.