1.
Chembiochem
; 13(4): 520-3, 2012 Mar 05.
Artigo
em Inglês
| MEDLINE
| ID: mdl-22275147
RESUMO
Inversion of stereoselectivity: screening of a minimal mutant library revealed a cytochrome P450 BM3 variant M01 A82W S72I capable of producing 16 α-OH-testosterone. Remarkably, a single active site mutation S72I in M01 A82W inverted the stereoselectivity of hydroxylation from 16 ß to 16 α. Introduction of S72I mutation in another 16 ß-OH-selective variant M11 V87I, also resulted in similar inversion of stereoselectivity.