RESUMO
Agri-food by-products, obtained as waste from the food industry, negatively impact the global economy and the environment. In order to valorize waste materials from fruit juices and tomato sauces as upcycled materials rich in health-promoting compounds, they were characterized in terms of polyphenolic and protein content. The results obtained were compared with those collected for their final products. The recovery of polyphenols was performed via ultrasound-assisted extraction (UAE). A high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed and validated to depict the quali-quantitative polyphenolic profile of both the by-products and the final products. The antioxidant capacity of the resulting extracts was characterized by UV-Vis spectrophotometric assays in terms of total phenolic content (TPC) and total antioxidant status (TAS). Moreover, the protein content was assessed with the Kjeldahl method too. The results highlighted a significant quantity of polyphenols remaining in peach, apricot, and apple by-products, which were able to exert an antioxidant activity (in the range of 4.95 ± 5.69 × 10-1 to 7.06 ± 7.96 × 10-1 mmol Trolox 100 g-1 of dry weight (DW) sample). Conversely, the tomato by-products were highly rich in proteins (11.0 ± 2.00 to 14.4 ± 2.60 g of proteins 100 g-1 DW). The results proved that all by-products may potentially be sustainable ingredients with nutritional and functional value in a circular bio-economy prospect.
RESUMO
Modifications at the glycolate moiety of englerin A were made to explore variations at the most sensitive site on the molecule for activity in the NCI 60 screen, wherein englerin A is highly potent and selective for renal cancer cells. Replacement of the glycolate by other functionalities as well as esterification of the glycolate hydroxyl yielded compounds which displayed excellent selectivity and potency compared with the natural product. TRPC4/5 ion channel experiments with five compounds showed delayed or reduced agonism with TRPC5, at much higher concentrations than englerin A. With TRPC4, these compounds all had no effect at 10 µM. The same compounds were not detectable in mouse serum after a single oral dose of 12.5 mg/kg. At 100 mg/kg p.o., no toxicity was observed, and blood levels were barely detectable. Intravenous administration led to toxicity but at substantially lower doses than for englerin A.
RESUMO
El Dr. Eduardo Sabas Alomá cursó la carrera de Medicina en la Universidad de La Habana y realizó estudios de postgrado en las universidades Harvard, Estados Unidos y San Marcos, Perú. Participó en actividades revolucionarias y políticas contra el gobierno del dictador Gerardo Machado que lo condujeron al exilio en los Estados Unidos, regresó a Cuba en 1937. Después del golpe de Estado de Batista en 1952, fue cesanteado por sus actividades contra el régimen. Profesor de Fisiología en la Facultad de Medicina de la Universidad de La Habana y profesor de Bacteriología de la Escuela de Enfermeras, ubicada en el Hospital Universitario "Calixto García", también trabajó como médico en el propio hospital y en Maternidad Obrera. Al triunfo revolucionario, permaneció en el claustro de profesores de la Escuela de Medicina, mientras que la mayoría renunciaba y marchaba a los Estados Unidos alentados por la contrarrevolución. Fundador del Instituto de Ciencias Básicas y Preclínicas "Victoria de Girón". Profesor Titular de Fisiología.
Dr. Eduardo Sabas Alomá studied Medicine in the University of Havana and carried out postgraduate studies at Harvard University, the United States of America and at the University of San Marcos in Peru. He participated in revolutionary and political activities against the government ruled by the dictator Gerardo Machado that led him to the exile in the United States. He returned to Cuba in 1937. After Batista´s coup d´état in 1952, he was interrupted because of his activities against the regime. He was professor of Physiology in the Medical Faculty of the University of Havana and professor of Bacteriology in the Nursing School located in "Calixto García" University Hospital; he also worked as a doctor in this hospital and in "Maternidad Obrera" Hospital. After the triumph of the Revolution, he was member of the Medical School teaching staff while most of the doctors renounced their professional work and went to the United States of America encouraged by the counterrevolution. He was founder of the Higher Institute of Basic and Pre-Clinical Sciences "Victoria de Girón" and Full Professor of Physiology
RESUMO
El Dr. Enrique Alberto Suárez Hernández matriculó en la Universidad de La Habana la carrera de Medicina en 1938 y se graduó en 1945. Ejerció como médico Municipal en Oriente, como médico forense en Banes, médico en Guanabacoa y profesor de Anatomía en la Escuela de Medicina de la Universidad de La Habana. En los años de la clandestinidad, colaboró con combatientes y campesinos que iban a verlo por problemas de salud. Al triunfo revolucionario se incorporó a las Milicias Nacionales Revolucionarias, fue fundador de los Comités de Defensa de la Revolución y miembro de la Central de trabajadores de Cuba. Participó en movilizaciones y trabajos voluntarios. Fundador del Instituto de Ciencias Básicas y Preclínicas "Victoria de Girón", Profesor Titular de Anatomía.
Dr. Enrique Alberto Suárez Hernández enrolled in the medical studies at the University of Havana in 1938 and graduated in 1945. He worked as Municipal Doctor in the East of Cuba, forensic doctor in the region of Banes, doctor in Guanabacoa, and Professor of Anatomy in the Medical School of the University of Havana. During the years of the clandestineness, he collaborated with combatants and farmers that went to see him with health problems. After the triumph of the Cuban Revolution, he became member of the National Revolutionary Militia. Dr. Enrique was one of the founders of the "Comités de Defensa de la Revolución" (CDR) and a member of the "Central de Trabajadores de Cuba" (CTC). He participated in mobilizations and voluntary works. Dr. Enrique Suárez Hernández was one of founders of the Institute of Basic and Pre-Clinical Sciences "Victoria de Girón"; full professor of Anatomy.
