The impact of prolonging extinction on the ABC "super renewal" of instrumental responses in rats.

Two free operant conditioning experiments with rats examined the impact of conducting a large amount of extinction training on situations that enhance the ABC renewal effect (ABC super renewal). In Experiment 1, ABC renewal was strengthened by conducting acquisition in multiple contexts. All rats were trained to press a lever for food. One group was trained in one context, while the other two groups were trained in three contexts. Then, all rats received extinction in context B. For two groups this phase lasted 4 sessions, whereas it lasted 36 sessions for the other group. In Experiment 2, ABC renewal was strengthened by using a large number of acquisition sessions. Rats were trained to perform an operant response to obtain food in context A. One group received a moderate amount of training, while the rest of the rats received a larger number of acquisition sessions. Responses underwent extinction in context B. Two groups received 4 sessions, while 36 extinction sessions were used for the remaining group. In both experiments, rats were tested in context B (extinction context) and C (renewal context). Greater ABC renewal occurred both when acquisition training was conducted in multiple contexts (Experiment 1) and by increasing the amount of acquisition training (Experiment 2). Nevertheless, we found that conducting a large number of extinction sessions reduced ABC super renewal in Experiment 1 only.

Analysis of Habituation Learning in Mealworm Pupae (Tenebrio molitor).

The decline of response as a consequence of repeated stimulation is known as habituation. The goal of the present experiments was extending the knowledge about habituation of abdominal contractions in the pupa of Tenebrio molitor. Both experiments consisted of two phases. During Phase 1, all groups were exposed to a continuous stimulus (light in Experiment 1 and vibration in Experiment 2). At the beginning of this phase, pupae showed a high number of abdominal contractions. However, during the last minute of Phase 1, the number of abdominal contractions was lower. In the next phase, the pupae were divided in different groups to test for response recovery. We found an increase in the abdominal contractions when subjects were exposed to a different stimulus, be it within the same or in a distinct sensory modality. In addition, we also reported response recovery when the pupae were re-exposed to the original stimuli after a resting period. Results indicate that the increase in responding cannot be explained by either sensory adaptation or fatigue. The findings are consistent with the perspective that suggests that habituation plays a major role in the survival of the species, even in non-feeding developmental stages.

Positive affective states can play the role of context to renew extinguished instrumental behavior in rats.

In one experiment with rats, we examined whether positive affective states can serve as contexts in a between-subjects ABA renewal design using appetitive instrumental conditioning. Two groups of rats received training to press a lever for food where each acquisition session was preceded by administration of a tickling procedure (Context A) known to induce positive affective states. Then, lever pressing underwent extinction where rats received a pure handling treatment (Context B) before each session. During a final test session, we found stronger responding when the session was preceded by tickling (Group ABA) compared to handling (Group ABB), indicating an ABA renewal effect. Furthermore, test performance in Group ABB was not different from that in a third group where handling preceded acquisition sessions, and tickling extinction and test sessions (Group BAA), showing that tickling did not elevate instrumental responding during the test if it had been unrelated to initial acquisition. We discuss implications of our results for understanding the role of positive affective states in relapse of problem behavior.

The impacts of acquisition and extinction cues on ABC renewal of voluntary behaviors.

In two instrumental conditioning experiments with rats, we examined the impacts of acquisition and extinction cues on ABC renewal of instrumental behavior. Animals were reinforced with food for lever pressing in one context, followed by extinction of the response in a second one. Presentations of a brief tone accompanied extinction in Experiment 1 (extinction cue), and acquisition in Experiment 2 (acquisition cue). A final test in a third context revealed that instrumental responding was decreased in the presence of the extinction cue, whereas it was increased in the presence of the acquisition cue. We discuss theoretical and clinical implications of our results.

Verbal retrieval cues prevent renewal and spontaneous recovery of predictive judgments in university students / Las señales de recuperación verbal evitan la renovación y la recuperación espontánea de juicios predictivos en estudiantes universitarios

Long-term effectiveness is one of the major problems of psychotherapy because successfully eliminated unhealthy behaviors may reappear relatively easily under certain contextual changes. In two experiments, we explored the impact of verbal retrieval cues on renewal and spontaneous recovery in humans. During the first phase, all participants learned a relationship between one cue and one outcome (X-O1). The following phase involved a change in the original relationship (X-O2). Finally, participants were presented with cue X and asked about the relationships with both outcomes. Half of the participants were asked to remember Phase 2, while the other half did not receive any instruction at all. In Experiment 1, testing took place in a different context, while participants in Experiment 2 were tested after two days. The results indicated that remembering Phase 2 eliminated both renewal and spontaneous recovery. Our findings are discussed under the contemporary learning theory. We also mention the probable benefits in therapeutic settings.

Uno de los principales problemas de las psicoterapias es su efectividad a largo plazo porque las conductas poco saludables que se eliminaron exitosamente pueden reaparecer con relativa facilidad bajo ciertos cambios contextuales. En dos experimentos, exploramos el impacto de señales de recuperación verbales en la renovación y en la recuperación espontánea en humanos. En la primera fase, todos los participantes aprendieron una asociación entre una clave y una consecuencia (X-O1). La siguiente fase involucró un cambio en la relación original (X-O2). Finalmente, se les presentó a los participantes la clave X y se les preguntó acerca de las asociaciones con las dos consecuencias. A la mitad de los participantes se les pidió que recordaran la Fase 2, mientras que la otra mitad no recibió ninguna instrucción. En el Experimento 1, la prueba se condujo en un contexto diferente, mientras que en el Experimento 2 la prueba se realizó dos días después. Los resultados indicaron que recordar la Fase 2 eliminó tanto la renovación como la recuperación espontánea. Nuestros hallazgos se discuten bajo la teoría contemporánea del aprendizaje. Asimismo, se mencionan probables beneficios para escenarios terapéuticos

Removing but not adding elements of a context affects generalization of instrumental responses.

Three experiments with rats investigated whether adding or removing elements of a context affects generalization of instrumental behavior. Each of the experiments used a free operant procedure. In Experiments 1 and 2, rats were trained to press a lever for food in a distinctive context. Then, transfer of lever pressing was tested in a context created either by adding an element to the context of initial acquisition or by removing one of the acquisition context's elements. In Experiment 3, a similar generalization test was conducted after rats received acquisition and extinction within the same context. For Experiments 1 and 2, we observed that removing elements from the acquisition context disrupted acquisition performance, whereas the addition of elements to the context did not. Experiment 3 revealed that removing elements from but not adding elements to the original context improved extinction performance. Our results are consistent with an elemental view of context representation.

Effects of extinction in multiple contexts on renewal of instrumental responses.

In two experiments with rats, we investigated the effects of using multiple contexts during extinction on renewal of lever-pressing behavior. During the first phase of both experiments, rats were reinforced to press a lever for food in Context A. Then, responses underwent extinction. For half of the animals, extinction sessions were conducted in a single context, whereas the other half received extinction in three different contexts. In Experiment 1, we observed that extinction in multiple contexts eliminated ABC renewal, but had no detectable impact on ABA renewal. Experiment 2 revealed that conducting extended extinction training in multiple contexts attenuated ABA renewal. Theoretical and clinical implications of the present findings are discussed.

A reminder of extinction reduces relapse in an animal model of voluntary behavior.

One experiment with rats explored whether an extinction-cue prevents the recovery of extinguished lever-pressing responses. Initially, rats were trained to perform one instrumental response (R1) for food in Context A, and a different instrumental response (R2) in Context B. Then, responses were extinguished each in the alternate context (R1 in Context B; R2 in Context A). For one group, extinction of both responses was conducted in the presence of an extinction-cue, whereas in a second group, the extinction-cue only accompanied extinction of R1. During a final test, we observed that returning the rats to the initial acquisition context renewed performance and that response recovery was attenuated in the presence of the cue that accompanied extinction of the response. The impact of the extinction-cue, however, was not transferred to the response that has been extinguished without the cue. Our results are consistent with the idea that extinction established an inhibitory cue-response association.

Context specificity of taste aversion is boosted by pre-exposure and conditioning with a different taste.

Recent reports in the literature show that an extinction treatment makes subsequently learned information context-specific. An experiment in conditioned taste aversion evaluated whether pre-exposure and conditioning with a given flavor would make conditioning of a different flavor context specific as well. Rats received conditioning with taste Y in context A, before being tested in extinction either in context A or in a different but equally familiar context (context B). Half of the animals received a pre-exposure and conditioning treatment with a different flavor (X), while the other half only received conditioning. The context change at testing led to higher consumption of Y in the animals that had received previous pre-exposure and conditioning with X. The implications of these results for the mechanisms underlying context-switch effects are discussed.

Extinction makes conditioning time-dependent.

Two experiments explored whether forgetting of an association depended on previous extinction of a different association in rats. Experiment 1 found that when rats were conditioned and extinguished with flavor X, a subsequently acquired conditioned aversion to flavor Y was reduced by a 19-day retention interval, something that did not occur when X and the US were initially presented unpaired. Experiment 2 found that when rats received training and extinction in one of two tasks (conditioned aversion to sucrose in Experiment 2a, and running for water in a straight alley in Experiment 2b), subsequent learning of the alternative task was partially forgotten over the 19-day retention interval. These results are similar to those previously found when manipulating physical and conceptual contexts in rats and humans, respectively, and suggest that the passage of time may play a role similar to the one played by the change in physical or conceptual contexts on information retrieval.

Context-switch Effect Produced by the Ambiguity of the Meaning of a Cue / Efecto de cambio de contexto producido por la ambigüedad del significado de la clave

One experiment analyzed whether context dependency of a flavor-illness association depends on the extinction of a different flavor-illness association in rats. There were two sessions per day, one in context A and the other session in context B. A half of the rats were allowed to drink distilled water within context B, while the other half of the group spent the same amount of time in context B without access to water. In context A, half of the subjects received conditioning and extinction of flavor X, while the other half did not received extinction. Then conditioning of flavor Y was conducted for all rats in context A. Finally, testing of Y was conducted in context A for half of the rats, while the other half received the test in context B. Results shown that extinction of flavor X affected the recovery of subsequently acquired information about flavor Y regardless the treatment received in context B. This data is consistent with Atttentional Theory of Context Processing.

Un experimento analizó si la dependencia contextual de una asociación sabor - enfermedad depende de la extinción de una asociación sabor - enfermedad distinta en ratas. Se realizaron dos sesiones diariamente, una en el contexto A y la otra en el contexto B. Una mitad del grupo ratas bebieron agua destilada en el contexto B, mientras que la otra mitad del grupo se colocó por el mismo tiempo en el contexto A sin beber nada. La mitad de las ratas recibieron condicionamiento y extinción de un sabor X en el contexto A, mientras que la otra mitad no recibió extinción. Luego todas las ratas fueron condicionadas con el sabor Y en el contexto A. Por ultimo, se hizo la prueba de Y en el contexto A para la mitad del grupo, y en el contexto B para la otra mitad. Los resultados mostraron que la extinción del sabor X afectó la recuperación del favor Y sin importer si el tratamiento se hizo en el contexto B. Estos datos son consistentes con la Teoría Atencional de Procesamiento del Contexto.

La generalización entre contextos como función del entrenamiento en una tarea instrumental con humanos / Generalization among Contexts as a Function of Training Humans in an Instrumental Task

León, Abad y Rosas, (2010b) demostraron que la atención al contexto se pierde conforme aumenta el entrenamiento. Así, a medida que aumenta el entrenamiento se observa una mayor generalización entre contextos distintos al de entrenamiento al perderse la atención. El objetivo del presente estudio fue poner a prueba ésta idea empleando una tarea instrumental en humanos con distintos valores del contexto (color). La tarea consistió en un juego de ordenador en el que se emitían distintas respuestas bajo un programa IV2s, en presencia de distintos estímulos discriminativos. En el Experimento 1 se entrenaron dos grupos con 3 y 8 ensayos, posteriormente se realizó un ensayo de prueba en el contexto de entrenamiento (amarillo) y en un contexto diferente (rojo). Los resultados demostraron una disminución de la ejecución con el cambio de contexto. En el Experimento 2 se entrenaron dos grupos de manera similar al Experimento 1, empleando un contexto naranja. Durante la prueba se presentaron degradaciones del color del contexto de entrenamiento con valores intermedios hacia el color amarillo y rojo. Los resultados sugieren que la atención al contexto de entrenamiento está modulada diferencialmente por la cantidad de entrenamiento en la tarea.

León, Abad and Rosas (2010b) shown that as the training increased attention to the context decreased. Thus, increasing the training might produce a greater generalization between different contexts. The main goal of the present experiments was to test this idea modifying different values of the context (i. e., colors) using an instrumental task with humans. The task was a videogame in which participants performed under an IV2s schedule in presence of different discriminative stimuli to obtain reinforces. In Experiment 1 two groups were trained with 3 or 8 trials, then a test trial was conducted in the training context (i. e., yellow) or in a different context (i. e., red). Results shown that changed the context produced a loss of performance. In Experiment 2 two groups were trained with similar parameters used in Experiment 1 but using an orange context. Test was conducted with different values of the orange (i. e., training context) toward yellow and red. The pattern of the data suggests that experience with the task modulates in different ways attention to contextual cues.

Modulación contextual en la extinción: recuperación de una aversión condicionada al sabor en un tercer contexto / Modulação contextual na extinção: recuperação de uma aversão condicionada ao sabor em um terceiro contexto / Contextual modulation of extinction: recovery of taste-aversion conditioning in a third context

Este experimento se diseñó para evaluar la renovación ABC con un procedimiento de condicionamiento de aversión al sabor (CAS) en tres fases: adquisición, extinción y prueba. Durante la adquisición, 15 ratas Wistar, asignadas a los grupos ABC y ABB, tuvieron acceso a una solución con sacarosa y posteriormente recibieron una inyección de cloruro de litio (liCl) en el Contexto A. En la extinción, ambos grupos bebieron la solución con sacarosa sin la administración del liCl en el Contexto B. En la prueba, los grupos tuvieron acceso a la solución con sacarosa sin administrarles liCl en el contexto de extinción para el grupo ABB, y en un tercer contexto para el grupo ABC. Los resultados mostraron la recuperación de la aversión condicionada al sabor únicamente en el grupo ABC.

This experiment was designed to evaluate ABC renewal in a conditioned taste aversion (CTA) procedure consisting of three phases: acquisition, extinction and test. During the acquisition phase, 15 Wistar rats assigned to groups ABC and ABB had access to a sucrose solution, after which they were injected with lithium chloride (liCl) in Context A. In the extinction phase, both groups of rats had access to the sucrose solution in Context B, but no liCl was administered. Finally, during the test, group ABB received sucrose solution without liCl in the extinction context, while group ABC was tested in a third context. Results showed renewal of conditioned taste aversion only in the ABC group.

Este experimento foi elaborado para avaliar a renovação ABC com um procedimento de condicionamento de aversão ao sabor (CAS) em três fases: aquisição, extinção e prova. Durante a aquisição, 15 ratos Wistar, designados aos grupos ABC e ABB, tiveram acesso a uma solução com sacarose e posteriormente receberam uma injeção de cloreto de lítio (liCl) em Contexto a. Na extinção, ambos grupos beberam a solução com sacarose sem a administração do liCl em Contexto B. Na prova, os grupos tiveram acesso à solução com sacarose sem administrar-lhes liCl num contexto de extinção para o grupo ABB, e num terceiro contexto para o grupo ABC. Os resultados mostraram a recuperação da aversão condicionada ao sabor unicamente no grupo ABC.

Effects of Scopolamine on Conditioning of Lever Pressing / Efectos de la escopolamina en el condicionamiento de presiones a la palanca

The aim of this work was to determine the effects of scopolamine, a cholinergic antagonist, on the conditioning of an instrumental response and the contextual conditioning of this response. Five groups of rats were trained to lever-press on a Variable Interval 30 s schedule in context A. Scopolamine was administered 15 min before each conditioning session to AB 0.01 mg/kg, AB 0.10 mg/kg and AB 1.00 mg/kg groups. The AA Saline and AB Saline groups received saline injections. Contextual conditioning of the lever-pressing response was assessed in one extinction session. The AA group received this extinction session in the conditioning context (A), while the AB groups received this session in a different context (B). Results showed that scopolamine impaired the conditioning of the lever-pressing response but no effects on contextual conditioning were found.

El propósito de este trabajo fue determinar los efectos de la escopolamina, un antagonista colinérgico, sobre el condicionamiento de una respuesta instrumental así como sobre el condicionamiento contextual de esta respuesta. Se entrenó a cinco grupos de ratas a presionar una palanca bajo un programa de Intervalo Variable 30 s en el contexto A. 15 min antes de cada sesión de condicionamiento se administró escopolamina en dosis de 0.01, 0.10 y 1.00 mg/kg a grupos diferentes en el contexto A, mientras dos grupos de ratas recibieron solución salina en el contexto A. El condicionamiento contextual de la respuesta de palanqueo se evaluó en una sesión de extinción en el contexto A o en un contexto B diferente. Los resultados mostraron que la escopolamina obstaculizó el condicionamiento de la respuesta de presionar la palanca pero no se encontraron efectos sobre el condicionamiento contextual.