RESUMO
The majority of condylomata acuminata (anogenital warts) are caused by infection with Human Papilloma Virus type 6 (HPV-6). We have sequenced the HPV-6 early genes, E1-E4, E6 and E7 from wart biopsy DNA samples sourced from the UK and USA and derived a consensus sequence for these genes and the proteins they encode. When compared to the prototype HPV-6b sequence, published over 12 years ago, the E1-E4 consensus sequence showed 3/91 (3.3%) amino acid changes, the E6 consensus sequence showed 1/150 (0.7%) changes and the E7 consensus sequence showed 1/98 (1.0%) changes. Since many of the early gene sequences from biopsy material were more similar to the HPV-6a subtype than HPV-6b, this data supports the use of HPV-6a as the HPV-6 prototype.
Assuntos
Deleção de Genes , Genes Virais , Papillomaviridae/genética , Proteínas Estruturais Virais/genética , Condiloma Acuminado/patologia , Condiloma Acuminado/virologia , Sequência Consenso , Análise Mutacional de DNA , DNA Viral/química , DNA Viral/genética , Humanos , Papillomaviridae/química , Reação em Cadeia da Polimerase , Proteínas Virais/genéticaRESUMO
In attempts to increase the immunogenicity of recombinant antigens, a number of particulate antigen presentation systems have been developed. In this study, we used human immunodeficiency virus Gag particles as carriers for the human immunodeficiency virus envelope V3 region. Gag:V3 fusion proteins were expressed from baculovirus expression vectors; they migrated to the insect cell membrane and budded from the cells as hybrid particles. An immunization study carried out with rats showed that the particles elicited a strong anti-Gag antibody response and a weak antibody response to the V3 region. A strong anti-V3 cytolytic T-cell response was elicited in immunized mice. These data show that retroviral Gag particles can be used as antigen presentation vehicles.
Assuntos
Síndrome da Imunodeficiência Adquirida/imunologia , Produtos do Gene env/imunologia , Produtos do Gene gag/imunologia , Anticorpos Anti-HIV/biossíntese , HIV/imunologia , Linfócitos T/imunologia , Sequência de Aminoácidos , Animais , Baculoviridae/genética , Sequência de Bases , Citotoxicidade Imunológica , Portadores de Fármacos , Produtos do Gene env/genética , Produtos do Gene gag/genética , HIV/genética , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Mariposas , Proteínas Recombinantes de Fusão/imunologiaRESUMO
The localization of neutralization determinants within the envelope glycoproteins of human immunodeficiency virus (HIV) has been largely achieved by immunizing small animals in conjunction with Freund's adjuvant. However, for eventual use in humans, candidate HIV vaccine components must also be efficacious in a nontoxic formulation. We describe here the production of hybrid Ty viruslike particles carrying the major neutralizing domain of HIV and demonstrate the induction of high-titer virus-neutralizing antibodies and an HIV-specific T-cell proliferative response after immunization in conjunction with aluminum hydroxide. As aluminum hydroxide and aluminum phosphate are the only adjuvants currently licensed for use in humans, these observations have implications for the development of an effective vaccine against HIV.
Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Anti-HIV/imunologia , Proteína gp120 do Envelope de HIV/imunologia , HIV/imunologia , Sequência de Aminoácidos , Animais , Formação de Anticorpos , Sequência de Bases , Sítios de Ligação de Anticorpos , Adjuvante de Freund , Vetores Genéticos , HIV/genética , Proteína gp120 do Envelope de HIV/genética , Proteína gp120 do Envelope de HIV/ultraestrutura , Dados de Sequência Molecular , Testes de Neutralização , Conformação Proteica , Coelhos/imunologiaAssuntos
Síndrome da Imunodeficiência Adquirida/imunologia , Anticorpos Antivirais/análise , Deltaretrovirus/imunologia , Homossexualidade , Antígenos de Diferenciação de Linfócitos T , Antígenos de Superfície/análise , Humanos , Hipersensibilidade Tardia/imunologia , Imunidade Celular , Contagem de Leucócitos , Linfócitos/classificação , Linfócitos/imunologia , Masculino , Monócitos/imunologia , FagocitoseAssuntos
Encefalite/complicações , Herpes Simples/complicações , Animais , Encéfalo/microbiologia , Doenças Desmielinizantes/etiologia , Encefalite/etiologia , Encefalite/patologia , Feminino , Herpes Simples/patologia , Camundongos , Nervos Periféricos/microbiologia , Doenças do Sistema Nervoso Periférico/etiologia , Simplexvirus/crescimento & desenvolvimento , Simplexvirus/isolamento & purificação , Fatores de Tempo , Ativação ViralRESUMO
The propagation of a cell line from a rabbit affected with the sheep associated form of malignant catarrhal fever is described. Immunological and morphological characteristics of the cell indicated that it was a T-lymphocyte and the presence of electron dense cytoplasmic granules suggested that the cell could be further classified as a large granular lymphocyte. The cell line required a feeder layer and was cytotoxic to both primary cell cultures and cell lines, a characteristic of large granular lymphocytes. No evidence of the nature of the agent could be detected but as few as 10(2) cells transmitted the disease. These findings are discussed and the possibility that infection and subsequent dysfunction of large granular lymphocytes may have a central role in the pathogenesis of malignant catarrhal fever is considered. That cells with similar characteristics have been derived from Herpesvirus saimiri and H ateles infected marmoset lymphocytes suggests that the lymphoproliferation associated with infection by these two simian herpes-viruses and malignant catarrhal fever may have a similar pathogenesis.
