Use of overpressurized thin-layer chromatography (OPTLC) for the separation of amino-acids and polypeptides.

Overpressurized thin-layer chromatography (OPTLC) has been used for the separation of amino-acids and polypeptides. The effects of the ratio of pyridine to acetic acid as well as that of butanol to water on the selectivity and efficiency of separation have been investigated in detail. The influence of the nature of organic solvents in the eluent has also been studied. The optimal conditions have been elaborated and the practical applicability in pharmaceutical analysis of the separation system has been demonstrated.