[Therapeutic-prophylactic milk products with a new immunocorrector of natural origin]. / Lechebno-profilakticheskie molochnye produkty s novym immunokorrektorom prirodnogo proiskhozhdeniia.

Authors had received and investigated on experiment milk medical and prophylactic products (milk and kefir) with addition peptide, obtained from nervous tissue of squids. It has been established that addition of gangliin to the milk and kefir causes stimulation cellular and humoral factors of immunity answer at laboratory animals. Medical and prophylactic milk products with gangliin return to the normal state reduced immunity indexes of the mice with experimental immunodeficits.

[The ecological aspects of listeriosis in the Maritime Territory]. / Ekologicheskie aspekty listerioza v Primorskom krae.

In the process of batch cultivation the strains under study are capable of prolonged growth at low temperature in rich and poor nutrient media (with the term of observation equal to 4 months), while at a temperature of 37 degrees C microbial populations quickly die (in 8-35 days). In the absence of compounds containing carbon, hydrogen and nitrogen in the nutrient medium, Listeria can proliferate under such conditions. As established with the use of gas chromatography and the radioisotopic method, they can uptake carbon dioxide, hydrogen and nitrogen from the air gas mixture, using carbon of the first gas for the synthesis of the main biopolymers (proteins, lipids, carbohydrates, DNA and RNA) and the second one as the source of energy. During the cultivation of Listeria at low temperature in poor nutrient media (soil microecosystems, synthetic mineral media) they are capable of preserving and under favorable conditions also increasing their virulence. Its increase is facilitated by capsule formation, mobility, chemotaxis, adhesion and invasion enhancing under such conditions.

[The diagnostic value of the polymerase chain reaction in tuberculosis]. / Diagnosticheskaia tsennost' polimeraznoi tsepnoi reaktsii pri tuberkuleze.

The comparative study of material obtained from tuberculosis patients in the Vladivostok Tuberculosis Dispensary was carried out with the use of polymerase chain reaction (PCR), the bacterioscopic and bacteriological methods. The study was made on different groups of tuberculosis patients (in the phase of the active course of the disease and after convalescence), patients with nonspecific pulmonary diseases and persons having contacts with tuberculosis patients. The study revealed that PCR was 2-3 times more effective than the other above-mentioned methods and made it possible to obtain results as early as 3-5 days after material was taken.

[Thermostable Yersinia pseudotuberculosis toxin: its isolation, purification and the characteristics of its properties]. / Termostabil'nyi toksin Yersinia pseudoterberculosis: vydelenie, ochistka, kharakteristika svoistv.

Heat-stable toxin, lethal for mice when administered parenterally (LD50 was equal to 4.5 micrograms/mouse), was isolated from Y. pseudotuberculosis grown at a temperature of 8-10 degrees C. The toxin was found to be species-specific protein with a molecular weight of 45 kD, resistant to the action of elevated temperature (boiling for 5 minutes), 0.1-1.0% anionic detergent (sodium dodecyl sulfate) and stable at the pH range between 6.0-8.0. The amino acid sequence of the N-end fragment of the toxin was determined: H2N-Ala-Glu-Val-Asp-Ser-Ser-Glu-Phen-Pro. The toxin was inactivated after treatment with trypsin, chemotrypsin, papain. Antibodies to this toxin were detected in the blood sera of pseudotuberculosis patients.

[Use of serum to thermostable toxin for identification of Yersinia pseudotuberculosis]. / Ispol'zovanie syvorotki k termostabil'nomu toksinu dlia identifikatsii Yersinia pseudotuberculosis.

Serum to thermostable Y. pseudotuberculosis toxin is shown to induce agglutination of bacteria belonging to all 8 serologic variants of this species. No cross reactions with other enterobacterial species, including four Yersinia species, were detected. The results permit us recommend the antitoxic serum for identification of Y. pseudotuberculosis.