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ACS Comb Sci ; 14(3): 145-9, 2012 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-22280455

RESUMO

A random hexapeptide library (one-bead-one-compound), containing sixteen amino acids (16(6) different sequences) was synthesized on a Tentagel resin previously modified with a dipeptide linker (Asp-Pro). This peptide bond is highly susceptible to cleavage under mild acidic conditions in a salt-free solution prepared with H(2)(16)O/H(2)(18)O (60/40% v/v). In the hydrolysis, hexapeptides are released with an additional Asp residue partially labeled with (18)O at the C-terminus. These conditions are fully compatible with ESI-MS analysis and facilitate sequencing by MS, as N- and C-terminal ions can be easily differentiated in MS/MS spectra. The peptides were sequenced manually and also with de novo sequencing programs, and identifying them in a database containing all possible heptapeptide sequences or in a filtered database. The proposed strategy is also compatible with stepwise Edman degradation using either intact beads or the released free peptides.


Assuntos
Dipeptídeos/química , Biblioteca de Peptídeos , Sequência de Aminoácidos , Técnicas de Química Combinatória , Dados de Sequência Molecular , Espectrometria de Massas por Ionização por Electrospray
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