RESUMO
Twenty-six bovine corneas, 30 micron thick, were sectioned perpendicular to the visual axis. Sections, weighing 10-20 mg, were analyzed for total water content by thermogravimetric analysis and for freezable water content by differential scanning calorimetry. The total water content as well as the free (freezable) water content increase, while the bound (nonfreezable) water content decreases in progressing from epithelium to endothelium. These results are correlated with the distribution of proteoglycans and their water sorptive and retentive capacity in bovine cornea.
Assuntos
Água Corporal/metabolismo , Córnea/metabolismo , Absorção , Animais , Bovinos , Sulfatos de Condroitina/metabolismo , Endotélio Corneano/metabolismo , Epitélio/metabolismo , Congelamento , Sulfato de Queratano/metabolismo , Distribuição TecidualRESUMO
Using polarized light scattered in the I'' and I+ modes, we determined the relative contributions of density and orientation fluctuation to the opacity of bovine lenses. In both the cortex and the nucleus of bovine lenses cultured in xylose column, we found that the increase in hydration effects the optical anisotropy (birefringence) first, before the lake formation will contribute heavily to the density fluctuation.
