RESUMO
UNLABELLED: In this study, yeasts and lactic acid bacteria (LAB) were isolated from coffee fruits and identified via biochemical and molecular approaches. The isolates represented the Pichia, Debaryomyces, Candida, Clavispora, Yarrowia, Sporobolomyces, Klyveromyces, Torulaspora and Lactobacillus genera. Four isolates, namely Pichia fermentans LPBYB13, Sporobolomyces roseus LPBY7E, Candida sp. LPBY11B and Lactobacillus brevis LPBB03, were found to have the greatest antagonist activity against an ochratoxigenic strain of Aspergillus westerdijkiae on agar tests and were selected for further characterization. Applications of P. fermentans LPBYB13 in coffee cherries artificially contaminated with A. westerdijkiae showed efficacy in reducing ochratoxin A (OTA) content up to 88%. These results highlight that P. fermentans LPBYB13 fulfils the principle requirements of an efficient biological control of aflatoxigenic fungi in coffee beans and may be seen as a reliable candidate for further validation in field conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: Studies based on microbial ecology and antagonistic interactions are important for the development of new strategies in controlling aflatoxin contamination of crops and are relevant to further biotechnological applications. This study shows that coffee fruit is a potential source for the isolation of microbial strains with antifungal ability. A new yeast strain, Pichia fermentans LPBYB13, showed efficacy in reducing growth and ochratoxin A production of Aspergillus westerdijkiae in coffee beans. Our results should encourage the use of this yeast strain on a large scale for biocontrol of aflatoxigenic fungi in coffee beans.
Assuntos
Aflatoxinas/biossíntese , Antifúngicos/isolamento & purificação , Aspergillus/crescimento & desenvolvimento , Agentes de Controle Biológico/isolamento & purificação , Café/microbiologia , Contaminação de Alimentos/prevenção & controle , Ocratoxinas/biossíntese , Agentes de Controle Biológico/metabolismo , Candida/isolamento & purificação , Candida/metabolismo , Frutas/microbiologia , Ácido Láctico/metabolismo , Levilactobacillus brevis/isolamento & purificação , Levilactobacillus brevis/metabolismo , Pichia/isolamento & purificação , Pichia/metabolismoRESUMO
We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.
Assuntos
Candida/isolamento & purificação , Solanum lycopersicum/microbiologia , Brasil , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 5,8S/genética , Análise de Sequência de DNARESUMO
We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.(AU)
Assuntos
Solanum lycopersicum/microbiologia , Candida parapsilosis , MicosesRESUMO
We aimed to isolate and identify yeasts found in the tomato fruit in order to obtain isolates with biotechnological potential, such as in control of fungal diseases that damage postharvest fruits. We identified Candida orthopsilosis strains LT18 and LT24. This is the first report of this yeast on Lycopersicum esculentum fruits in Brazil.
Assuntos
Candida parapsilosis , Solanum lycopersicum/microbiologia , MicosesRESUMO
This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.
Assuntos
Microbiologia de Alimentos/métodos , Listeria/isolamento & purificação , Salmonella/isolamento & purificação , Ensaio de Imunoadsorção Enzimática/métodos , Programas de Rastreamento/métodos , Sensibilidade e EspecificidadeRESUMO
This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.(AU)
Assuntos
Listeria , Técnica de Imunoensaio Enzimático de Multiplicação , Salmonella , BenchmarkingRESUMO
This study was carried out comparing the conventional methods (ISO 11290-1 and BAM method, 2008) and system mini-Vidas® (Biomerieux), for detection of Listeria sp. and Salmonella sp. in cooled sausage. The immunoenzymatic method has shown to be effective for the detection of target pathogens, it has presented itself as an excellent screening method.