RESUMO
Current American Dental Association infection control guidelines recommend immersion disinfection of irreversible hydrocolloid impressions, and this study further defines the parameters for use of sodium hypochlorite. Sodium hypochlorite has been shown to be an effective disinfectant for impressions; however, it has not been fully evaluated for optimum immersion time and concentration. In this study, irreversible hydrocolloid impressions contaminated with different bacteria were immersed in varying concentrations of sodium hypochlorite for 1, 5, or 10 minutes. Dilute solutions of sodium hypochlorite (0.525% or 0.0525%) produced a 4-log10 (99.99%) reduction in colony-forming units of Staphylococcus aureus, Salmonella choleraesuis, or Pseudomonas aeruginosa after 1 to 5 minutes' immersion. Full-strength sodium hypochlorite (5.25%) required 5 minutes to produce a 4-log10 reduction of Bacillus subtilis. A 4-log10 reduction of Mycobacterium bovis was not obtained under any conditions examined.
Assuntos
Bactérias/efeitos dos fármacos , Materiais para Moldagem Odontológica , Desinfecção/métodos , Contaminação de Equipamentos , Hipoclorito de Sódio/farmacologia , Alginatos , Bacillus subtilis/efeitos dos fármacos , Contagem de Colônia Microbiana , Imersão , Infecção Laboratorial/prevenção & controle , Testes de Sensibilidade Microbiana , Mycobacterium bovis/efeitos dos fármacos , Pseudomonas aeruginosa/efeitos dos fármacos , Salmonella/efeitos dos fármacos , Esporos Bacterianos/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
Thermostable antigen (TA) occurs in ovarian carcinoma and in certain specialized tissues. High titers of TA were found in nineteen of twenty endocervical extracts but not in myometrium, endometrium, or exocervix. TA was present in all of sixteen cervical mucus samples. Antibodies to perchloric acid extract of bronchus showed cross-reaticity with TA from ovarian carcinoma and cervix. Immunofluorescence with frozen sections revealed TA to be present in the columnar epithelium of ovarian neoplasms, and endocervical glands, in bronchial seromucous glands and in hepatic bile canaliculi. Immunofluorescence and immunodiffusion were used to test for cancer patient antibodies against TA. All sera tested negative. An immunofluorescence inhibition test for circulating TA also tested negative in sera of ovarian and cervical cancer patients.
Assuntos
Antígenos de Neoplasias , Neoplasias Ovarianas/imunologia , Adulto , Idoso , Animais , Anticorpos Antineoplásicos , Ductos Biliares Intra-Hepáticos/imunologia , Brônquios/imunologia , Gatos , Colo do Útero/imunologia , Reações Cruzadas , Cães , Epitopos , Feminino , Cobaias , Haplorrinos , Humanos , Técnicas Imunológicas , Pessoa de Meia-Idade , Muco/imunologia , Especificidade de Órgãos , Coelhos , Ratos , Especificidade da EspécieRESUMO
Five tissue antigens have been identified which appear in carcinoma arising in the ovary. Antisera to a variety of human tissues were absorbed with a saline extract of normal ovary as well as plasma; five distinct antigens (A1-A4, TA) were detected in ovarian tumor extracts as well as in normal tissues. A1, A2, and A3 were widely distributed in normal tissues while A4 was found principally in buffy coat blood cells and spleen, and TA was mainly associated with normal cervix but was variably detected in liver and lung. Heating the tumor extract to 56 degrees resulted in inactivation of A1, A2, and A3. A4 was inactivated at 80 degrees, whereas TA was stable at 100 degrees. Pronase treatment readily abolished A3 activity, more slowly inactivated A1, A2, and A4, and only very slowly destroyed TA. Partial separation of the antigens was obtained by Sephadex G-200 chromatography. TA from individual benign and malignant tumors as well as normal cervix appears to be the same molecule by criteria of immunodiffusion, immunoelectrophoresis, and gel filtration. The potential use of these antigens as tracers of ovarian carcinoma is indicated.
