RESUMO
The specific polysaccharide antigen of Streptococcus pneumoniae serotype 35A was shown, by a combination of one- and two-dimensional NMR methods and chemical analyses, to be a high-molecular-mass polymer composed of D-galactose, D-glucose, mannitol, and phosphate (3:1:1:1). The pentasaccharide repeating unit is polymerized through phosphate diester linkages to give the structure, [formula in text] O-Acetyl substituents are present at positions 5 and 6 of the 3)-beta-D-Galf residue and at position 2 of the 6)-beta-D-Galf residue. The capsular polysaccharides of S. pneumoniae serotypes 20 and 35A both contain the disaccharide unit -->3)-beta-D-Galf-(1-->3)-beta-D-Glcp-(1--> which is the probable structural determinant responsible for the serological cross reactivity of the two polysaccharides.
Assuntos
Cápsulas Bacterianas/imunologia , Polissacarídeos Bacterianos/imunologia , Streptococcus pneumoniae/imunologia , Cápsulas Bacterianas/química , Configuração de Carboidratos , Sequência de Carboidratos , Reações Cruzadas , Dissacarídeos/análise , Dissacarídeos/imunologia , Epitopos/análise , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , Oligossacarídeos/análise , Oligossacarídeos/imunologia , Polissacarídeos Bacterianos/química , Sorotipagem , Streptococcus pneumoniae/classificaçãoRESUMO
The O antigen of the lipopolysaccharide of Burkholderia cepacia serotype E (O2) was shown by a combination of methylation analysis, partial hydrolysis, NMR, and mass spectrometric methods to be a high molecular weight polysaccharide composed of two different trisaccharide repeating units in the ratio 2:1. The major trisaccharide component is composed of two alpha-D-mannopyranosyl and one beta-D-galactopyranosyl residues with the structure, [-->2)-alpha-D-Man p-(1-->2)-alpha-D-Man p-(1-->4)-beta-D-Gal p-(1-->]n The minor trisaccharide component is a D-mannan composed of two alpha- and one beta-D-mannopyranosyl residues with the structure, [-->2)-alpha-D-Man p-(1-->2)-alpha-D-Man p-(1-->3)-beta-D-Man p-(1-->]n
Assuntos
Antígenos O/química , Pseudomonas/química , Trissacarídeos/química , Configuração de Carboidratos , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , Estrutura Molecular , Pseudomonas/classificação , SorotipagemRESUMO
The O antigen obtained from the lipopolysaccharide of Yersinia ruckeri serotype 01, by mild acid hydrolysis, is composed of a branched tetrasaccharide repeating unit containing 2-acetamidino-2,6-dideoxy-L-galactose (L-FucAm), 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), and 7-acetamido-3,5,7,9-tetradeoxy-5-(4-hydroxybutyramido)-D-glycero-L -galacto- nonulosonic acid (L-Sug). Partial hydrolysis of the O antigen with 0.1 M HClafforded a trisaccharide and a tetrasaccharide having nonulosonic acid at their reducing ends. Cleavage of the O antigen with anhydrous methanolic hydrogen fluoride afforded the methyl glycoside derivatives of a trisaccharide and a tetrasaccharide. 1H and 13C NMR analysis, including 1H-13C heteronuclear multiple bond correlation spectroscopy to locate the N-acyl substituents, together with mass spectrometric analysis of the above oligosaccharides, allowed the structure of the O-specific polysaccharide to be assigned as: [formula: see text].
Assuntos
Lipopolissacarídeos/química , Oligossacarídeos/química , Polissacarídeos Bacterianos/química , Yersinia/química , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Antígenos O , Análise de Sequência , Sorotipagem , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Yersinia/classificaçãoRESUMO
The O-antigenic polysaccharides of the lipopolysaccharides produced by three isolates of Pseudomonas cepacia serogroup A were analysed by composition, methylation, periodate oxidation, and two-dimensional nuclear magnetic resonance methods. They were found to be identical linear unbranched polymers of a repeating trisaccharide unit containing 2-acetamido-2-deoxy-D-galactose and L-rhamnose residues (2:1) and have the structure [-->3)-alpha-D-GalpNAc-(1-->3)-beta-D-GalpNAc-(1-->4)-alpha-L-Rhap -(1-->]n.
Assuntos
Lipopolissacarídeos/química , Polissacarídeos Bacterianos/química , Pseudomonas/imunologia , Configuração de Carboidratos , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , Antígenos O , Oxirredução , Sequências Repetitivas de Ácido NucleicoRESUMO
The capsular antigen of Actinobacillus pleuropneumoniae serotype 11 was characterized by one-dimensional high-field nuclear magnetic resonance methods and chemical analyses, as a teichoic-acid-type polymer composed of a repeating unit with the structure [see text].
Assuntos
Actinobacillus pleuropneumoniae/imunologia , Cápsulas Bacterianas/química , Actinobacillus pleuropneumoniae/química , Cápsulas Bacterianas/isolamento & purificação , Sequência de Carboidratos , Cromatografia em Gel , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , PolímerosRESUMO
The specific capsular antigen of Actinobacillus pleuropneumoniae serotype 9 was characterized by one-dimensional and two-dimensional high-field nuclear-magnetic-resonance methods, and by chemical analyses, as a teichoic-acid-type polymer of a repeating unit having the structure [formula: see text] The basic polymer structure is identical to capsular antigens of Neisseria meningitidis group H, Escherichia coli K62 (K2ab) and Pasteurella haemolytica serotype T15.
Assuntos
Actinobacillus/imunologia , Antígenos de Bactérias/química , Escherichia coli/imunologia , Mannheimia haemolytica/imunologia , Neisseria meningitidis/imunologia , Reações Cruzadas , Imunodifusão , Espectroscopia de Ressonância MagnéticaRESUMO
The antigenic lipopolysaccharide O polysaccharides of capsular serotypes 9 and 11 were examined by chemical, immunological, and nuclear magnetic resonance methods. Immunodiffusion tests carried out on these O antigens indicated that both contained common epitopes which were also shared by Actinobacillus pleuropneumoniae serotype 1. Chemical analysis and high-field nuclear magnetic resonance spectroscopy showed that the O antigens of serotypes 9 and 11 were high-molecular-weight polymers consisting of a backbone of repeating trisaccharide units composed of alpha-L-rhamnopyranosyl and alpha-D-glucopyranosyl residues (2:1). One of the alpha-L-rhamnose units forms a branch point and is stoichiometrically substituted with terminal 2-acetamido-2-deoxy-beta-D-glucose residues in the serotype 11 O polysaccharide, but only to the extent of 25% in the serotype 9 O polysaccharide. Thus, the serotype 9 O polysaccharide contains two different repeating units: a tetrasaccharide unit with the same structure as that of the serotype 11 O polysaccharide and a trisaccharide unit: [formula: see text] where R = beta-D-GlcpNAc for serotype 1 and 11 O polysaccharides, and R = H (75%) and R = beta-D-GlcpNAc (25%) for serotype 9. The structure of the previously determined serotype 1 O polysaccharide (E. Altman, J.-R. Brisson, and M. B. Perry, Biochem. Cell. Biol. 64:17-25, 1986) is identical to that of the serotype 11 O polysaccharide. We propose a more complete serotyping scheme for A. pleuropneumoniae which includes designation of both the capsular (K) and O antigens.
Assuntos
Actinobacillus pleuropneumoniae/imunologia , Polissacarídeos Bacterianos/análise , Actinobacillus pleuropneumoniae/classificação , Sequência de Carboidratos , Eletroforese em Gel de Poliacrilamida , Epitopos/imunologia , Imunodifusão , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , Antígenos O , Polissacarídeos Bacterianos/imunologia , SorotipagemRESUMO
Proteus mirabilis 2573 (ATCC 49565) produces an acidic capsular polysaccharide which was shown from glycose analysis, carboxyl reduction, methylation, periodate oxidation, and the application of one dimensional and two-dimensional high-resolution nuclear magnetic resonance techniques to be a high-molecular-weight polymer of branched trisaccharide units composed of 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine), 2-acetamido-2,6-dideoxy-L-galactose (N-acetyl-L-fucosamine), and D-glucuronic acid, having the structure: [formula: see text] P. mirabilis 2573 also produces an O:6 serotype lipopolysaccharide in which the O-chain component has the same structure as the homologous capsular polysaccharide. This is the first report of a defined capsular polysaccharide in this bacterial genus.
Assuntos
Polissacarídeos Bacterianos/química , Proteus mirabilis/ultraestrutura , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência MolecularRESUMO
The capsular polysaccharide of A. pleuropneumoniae serotype 10 was found to be composed of a linear disaccharide repeating unit. By use of methylation analysis, partial hydrolysis, g.l.c.--and f.a.b.- m.s., and 1D and 2D n.m.r. studies, the polysaccharide was determined to be a polymer of a repeating disaccharide unit composed of D-mannose and 3-deoxy-D-manno-octulosonic acid (Kdo) having the structure: [formula; see text]
Assuntos
Actinobacillus pleuropneumoniae/química , Cápsulas Bacterianas/química , Polissacarídeos Bacterianos/química , Sequência de Carboidratos , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , Polissacarídeos Bacterianos/isolamento & purificação , Sorotipagem , Soluções/químicaRESUMO
The capsular polysaccharide of A. pleuropneumoniae is composed of 2-acetamido-2-deoxy-D-glucose (3 parts) and phosphate (1 part). The arrangement of these components in the repeating unit was determined by dephosphorylation, methylation, g.l.c.-m.x., and 1D and 2D n.m.r. spectroscopic methods. The polysaccharide was found to be a high molecular weight polymer of repeating trisaccharide units, joined through phosphate diester linkages, having the structure, (formula: see text).
Assuntos
Actinobacillus pleuropneumoniae/química , Cápsulas Bacterianas/química , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura Molecular , FosforilaçãoRESUMO
The structure of the O-antigen polysaccharide of A. pleuropneumoniae serotype 7 was investigated by methylation analysis, partial acid hydrolysis, periodate oxidation, and 1H- and 13C-n.m.r. spectroscopy. The polysaccharide repeating-unit consists of a branched tetrasaccharide having the following structure. [formula: see text]
Assuntos
Actinobacillus/análise , Antígenos de Bactérias/química , Lipopolissacarídeos/química , Actinobacillus/classificação , Actinobacillus/imunologia , Configuração de Carboidratos , Sequência de Carboidratos , Lipopolissacarídeos/imunologia , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura Molecular , Antígenos O , SorotipagemRESUMO
The structure of the capsular polysaccharide antigen of A. pleuropneumoniae serotype 7 was determined using 1D- and 2D-n.m.r. methods. Dephosphorylation, methylation, and g.l.c.-mass spectrometry methods were used to confirm the analysis. The type-7 specific polysaccharide was a high-molecular-weight teichoic acid-type polymer of D-galactose, glycerol, and phosphate (2:1:1), composed of glycosylglycerol repeating units joined through monophosphate diester linkages and with the structure: [formula: see text]
Assuntos
Actinobacillus/análise , Polissacarídeos Bacterianos/química , Actinobacillus/classificação , Actinobacillus/imunologia , Antígenos de Bactérias/química , Cápsulas Bacterianas , Configuração de Carboidratos , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Estrutura Molecular , Polissacarídeos Bacterianos/imunologia , SorotipagemRESUMO
The structure of the capsular antigen of E. coli K49 and the oligosaccharides derived from it by partial acid hydrolysis were studied by 1D- and 2D-n.m.r. spectroscopy, g.l.c.-c.i.-mass spectrometry, and methylation analysis. The K49 polysaccharide consists of the repeating unit----4)-beta-D-GlcpA-(1----6)-beta-D-Galp-(1----6)-beta-D-Glcp- (1----3)-beta - D-GalpNAc-(1----. The glucuronic acid residues are substituted, in the apparent molar ratio of 4:1, with L-threonine and L-serine linked amidically to the carboxyl group.
Assuntos
Polissacarídeos Bacterianos/química , Antígenos de Bactérias/química , Cápsulas Bacterianas , Sequência de Carboidratos , Escherichia coli/análise , Escherichia coli/imunologia , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Polissacarídeos Bacterianos/imunologiaRESUMO
The structure of the capsular antigen of E. coli K26 has been found by a combination of chemical and spectroscopic techniques to be of the "5 + 1" type shown. An important step was the simultaneous separation and identification of a mixture of neutral and acidic oligosaccharides by g.l.c.-c.i.-m.s. [formula: see text]