[The modern characteristics of occupational morbidity of agriculture workers].

On the basis of updated data from 82 subjects of the Russian Federation multivariate analysis of occupational diseases of agricultural workers was implemented. It is established that in unhealthy work conditions are characteristic for 30% of workers in the agricultural sector. In the enterprises of agriculture, the percentage of workplaces not meeting sanitary hygienic standards made up to 35%. The high etiologic pathogenic relationship between nosological form of disease and working conditions in the profession is established. Most often, the occupational pathology was developed in agricultural workers in case of conditions of physical overload (37.2%), effecting of physical factors (26.8%) and contact with infected animals (14.6%). In nosological structure of accumulated occupational diseases the first place took radiculopathy of cervical and lumbar sacral levels (23%), the second - vibration disease (15.3%), followed by brucellosis (14.6%), the fourth - neuro-sensory deafness (11.5%). The regions of the Russian Federation are characterized by pronounced heterogeneity both related to general level of registered occupational diseases and its nosological spectrum. In the whole, in Russia during 2011-2015 persistent trend of decreasing of occupational diseases morbidity of agricultural workers was marked.

[Hygienic characteristics of work conditions and health state parameters in workers of chemical enterprise complex].

The authors presented hygienic characteristics of industrial processes in chemical industrial complex (methacrylic acid, sodium cyanide) and results of work conditions influence on workers' health state through periodic medical examinations data.

[Effect of blood coagulation on the functional activity of transport adenosine triphosphatases in erythrocytes]. / Vliianie svertyvaniia krovi na funktsional'nuiu aktivnost' transportnykh adenozintrifosfataz éritrotsitov.

Membranes of red blood cells from the whole blood and the blood containing anticoagulant were comparatively studied in 30 donors. It was shown that their destabilization during blood coagulation in vitro was caused by a decrease in the quantity of sulfhydryl groups in red blood cell lipoprotein complexes that deteriorated not only structural but also functional state of erythrocytic membranes due to the changes in the activity of their transport ATPases.

[Free radical oxidation of red blood cell membrane lipid structures as a trigger mechanism of an increase in red blood cell membrane permeability during blood coagulation in vitro]. / Svobodnoradikal'noe okislenie lipidnykh struktur membran éritrotsitov kak puskovoi mekhanizm povysheniia membrannoi pronitsaemosti krasnykh kletok krovi pri ee svertyvanii in vitro.

The study of venous blood samples from 50 clinically normal subjects has shown that free radical oxidation of red blood cell membrane lipid structure induced by the blood sample contact with atmospheric oxygen serves a trigger rising the membrane permeability during blood coagulation in vitro. Activation of endogenous phospholipase A2 is a process dependent on the increase of intraerythrocytic concentration of calcium ions.

[The effect of molecular oxygen and calcium ions on the membrane permeability of erythrocytes]. / Vliianie molekuliarnogo kisloroda i ionov kal'tsiia na membrannuiu pronitsaemost' éritrotsitov.

Extracellular Ca has shown the highest membrane destroying effect of all the factors studied. Its injurious action augmented when blood samples were exposed to atmospheric oxygen. To improve the diagnostic value of biochemical studies at the expense of red cell membrane stabilization the authors recommend the use of blood citrate plasma as the basic object of enzymic studies.

[A study of the enzymes in citrated blood plasma]. / Issledovanie fermentov v tsitratnoi plazme krovi.

Elevated blood serum, vs. the plasma, levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase activities are explained by these enzymes discharge from red cells into the liquid fraction of the blood during its in vitro coagulation. To eliminate the "tube" hemolysis, the authors recommend measurements of these enzymes activities in the blood citrate plasma.

[Activation of lipid peroxidation in erythrocytes during blood coagulation in vitro]. / Aktivatsiia protsessov perekisnogo okisleniia lipidov v éritrotsitakh pri svertyvanii krovi in vitro.

A comparative investigation of antioxidant enzyme activity and lipid hydroperoxide concentration in red blood cells of the whole and anticoagulant-containing blood of 33 donors was conducted to elucidate the causes of increasing the permeability of red blood cell membrane during blood coagulation in vitro. It has been shown that one of the causes of intensified red blood cell peroxidation during blood coagulation is a disturbed ratio of superoxide dismutase and catalase activities leading to a decrease in the antioxidant enzymatic protection.

[Destabilization of erythrocyte lipid structures in blood coagulation]. / Destabilizatsiia lipidnykh struktur éritrotsitov pri svertyvanii krovi.

Main parameters of lipid complex were studied in erythrocytes of whole blood and of the blood containing anticoagulant. Initial steps of blood coagulation involved activation of erythrocyte endogenous phospholipase A, which led to destabilization of erythrocyte lipid structures as a result of an increase in concentration of free fatty acids, accumulation of lysophospholipids as well as of alterations in microviscosity of erythrocyte membranes.

[Changes in the membrane permeability of erythrocytes during blood coagulation in vitro]. / Izmenenie membrannoi pronitsaemosti éritrotsitov pri svertyvanii krovi in vitro.

It was established that permeability of erythrocyte membranes increases significantly during coagulation of blood in vitro. As a result, a certain amount of biosubstrates may pass into blood plasma from the erythrocytes. The increase of red cell membrane permeability in coagulation of blood is due to activation of free-radical oxidation of the cell lipid structures.

[Comparative effectiveness of intracoronary and systemic thrombolytic therapy of acute myocardial infarction]. / Sravnite'lnaia otsenka éffektivnosti intrakoronarnoi i sistemnoi tromboliticheskoi terapii ostrogo infarkta miokarda.

Systemic thrombolytic therapy with streptodekase was shown to produce the same effects as intracoronary thrombolytic therapy with avelysine. The blood flow in the coronary vessels could be restored in 72.7% with intracoronary therapy and in 66.7% with systemic thrombolytic therapy. Death rates were 9.1 and 8.9%, respectively, and in 3.1 and 3.3% with reperfusion. A difference was found in the frequency of myocardial infarction-associated complications, depending on the modality of thrombolytic therapy. In the authors' opinion, in clinical institutions where operations on coronary vessels are not supposed to be made after thrombolytic therapy, the systemic thrombolytic therapy with streptodekase should be preferred as it is simplier, safer and easier to apply.

[A method of determining the phospholipase activity of erythrocytes]. / Metod opredeleniia fosfolipaznoi aktivnosti éritrotsitov.

A method for measuring the activity of the red cell endogenous phospholipase A is suggested, consisting in red cell destruction, extraction of erythrocytic phospholipase, and detection of the enzymic activity by phosphatidylcholine hydrolysis in the organic phase from the degree of clarification of the lecithin emulsion.

[Factors contributing to the success of intracoronary thrombolytic therapy]. / Faktory, vliiaiushchie na uspekh intrakoronarnoi tromboliticheskoi terapii.

Intracoronary thrombolytic therapy (ICT) was used in 36 patients with acute transmural myocardial infarction. Recanalization of an infarction-dependent artery was achieved in 26 (72.2%) patients. Factors affecting the success of ICT were analysed, using Bayes' theorem. The ejection fraction (EF) is shown to have the highest predictive value as an indicator of left-ventricular pump function. The anticipated probability of ICT success is 84.9% in patients with acute myocardial infarction and a 35% EF baseline, and 25.7% in those with a 35% EF. In the absence of severe left-ventricular pumping and contractility disorders, the success of ICT is affected by patient's age, history of myocardial infarction and the site of thrombotic occlusion.