1.
Prikl Biokhim Mikrobiol
; 38(1): 29-34, 2002.
Artigo
em Russo
| MEDLINE
| ID: mdl-11852563
RESUMO
Nine Gram-negative bacterial strains, selected from 300 strains isolated from soils of the West Siberian petroliferous basin and growing on oil and oil products, consume phenol as a single carbon and energy source. The strains were used for the development of a sensor bioreceptor. The most active 32-I strain was shown to bear a plasmid responsible for phenol degradation. The plasmid-free derivative of this strain, 32-I-1, did not grow on phenol. The possibility of creating a model biosensor for phenol based on the plasmid-containing 32-I strain is considered. The detection limit for phenol was 5 microM. The optimum conditions for the sensor operation are: pH 7.4, 35 degrees C, and operation time 30 h.