Copper nanowire embedded hypromellose: An antibacterial nanocomposite film.

The present work reports a novel antibacterial nanocomposite film comprising of copper nanowire impregnated biocompatible hypromellose using polyethylene glycol as a plasticiser. Detailed physico-chemical characterization using X-ray diffraction, Fourier transform infrared spectroscopy, UV-Visible spectroscopy and electron microscopy shows uniform dispersion of copper nanowire in the polymer matrix without any apparent oxidation. The film is flexible and shows excellent antibacterial activity against both Gram positive and negative bacteria at 4.8 wt% nanowire loading with MIC values of 400 µg/mL and 500 µg/mL for E. coli and S. aureus respectively. Investigation into the antibacterial mechanism of the nanocomposite indicates multiple pathways including cellular membrane damage caused by released copper ions and reactive oxygen species generation in the microbial cell. Interestingly, the film showed good biocompatibility towards normal human dermal fibroblast at minimum bactericidal concentration (MBC). Compared to the copper nanoparticles reported earlier in vitro studies, this low cytotoxicity of copper nanowires is due to the slow dissolution rate of the film and production of lower amount of ROS producing Cu2+ ions. Thus, the study indicates a strong potential for copper nanowire-based composites films in broader biomedical and clinical applications.

Impact of metallic nanoparticles on anaerobic digestion: A systematic review.

Anaerobic digestion (AD) is one of the most energy-efficient waste treatment technologies for biodegradable wastes. Owing to the increasing trend of metallic nanoparticle applications in industry, they are ubiquitous to the waste streams, which may lead to remarkable impacts on the performance of the AD process. This review addresses the knowledge gaps and summarises the findings from the academic articles published from 2010 to 2019 focusing on the influences on both AD processes of biochemical hydrogen-generation and methane-production from selected metallic nano-materials. Both qualitative and quantitative analyses were conducted with selected indicators to evaluate the metallic nanoparticles' influences on the AD process. The selected metallic nanoparticles were grouped in the view of their chemical formulations aiming to point out the possible mechanisms behind their effects on AD processes. In summary, most metallic nanoparticles with trace-element-base (e.g. iron, cobalt, nickel) have positive effects on both AD hydrogen-generation and methane-production processes in terms of gas production, effluent quality, as well as process optimisation. Within an optimum concentration, they serve as key nutrients providers, aid key enzymes and co-enzymes synthesis, and thus stimulate anaerobic microorganism activities. As for the nano-additives without trace-element base, their positive influences are relied on providing active sites for the microorganism, as well as absorbing inhibitory factors. Moreover, comparisons of these nano-additives' impacts on the two gas-production phases were conducted, while methane-production phases are found to be more sensitive to additions of these nanoparticles then hydrogen-production phase. Research perspectives and research gaps in this area are discussed.

A Light-Activated Antimicrobial Surface Is Active Against Bacterial, Viral and Fungal Organisms.

Evidence has shown that environmental surfaces play an important role in the transmission of nosocomial pathogens. Deploying antimicrobial surfaces in hospital wards could reduce the role environmental surfaces play as reservoirs for pathogens. Herein we show a significant reduction in viable counts of Staphylococcus epidermidis, Saccharomyces cerevisiae, and MS2 Bacteriophage after light treatment of a medical grade silicone incorporating crystal violet, methylene blue and 2 nm gold nanoparticles. Furthermore, a migration assay demonstrated that in the presence of light, growth of the fungus-like organism Pythium ultimum and the filamentous fungus Botrytis cinerea was inhibited. Atomic Force Microscopy showed significant alterations to the surface of S. epidermidis, and electron microscopy showed cellular aggregates connected by discrete surface linkages. We have therefore demonstrated that the embedded surface has a broad antimicrobial activity under white light and that the surface treatment causes bacterial envelope damage and cell aggregation.