RESUMO
A water soluble heteroglycan, isolated from the alkaline extract of the fruit bodies of the somatic hybrid mushroom (PfloVv1aFB), raised through protoplast fusion between the strains of Pleorutus florida and Volverilla volvacea, was found to consist of (1â3)-, (1â6)-, (1â3,4)-linked, and terminal ß-D-Glcp along with (1â2,6)-α-D-Galp and terminal α-D-Manp in a relative proportion of approximately 1:1:1:1:1:1. This polysaccharide exhibited strong immunostimulating activity of macrophages as well as splenocytes and thymocytes. Structural investigation was carried out using sugar analysis, methylation analysis; periodate oxidation study, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of the above mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: [formula see text].
Assuntos
Adjuvantes Imunológicos/química , Adjuvantes Imunológicos/farmacologia , Pleurotus/química , Polissacarídeos/química , Polissacarídeos/imunologia , Volvariella/química , Adjuvantes Imunológicos/isolamento & purificação , Álcalis/química , Animais , Configuração de Carboidratos , Proliferação de Células/efeitos dos fármacos , Relação Dose-Resposta a Droga , Frutas/química , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Camundongos , Pleurotus/imunologia , Polissacarídeos/isolamento & purificação , Espécies Reativas de Oxigênio/imunologia , Baço/citologia , Baço/efeitos dos fármacos , Baço/imunologia , Relação Estrutura-Atividade , Timócitos/efeitos dos fármacos , Timócitos/imunologia , Volvariella/imunologiaRESUMO
A water-soluble glucan, isolated from the alkaline extract of the fruit bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to consist of (1â3,6)-linked, (1â3)-linked, (1â6)-linked, and terminal ß-d-glucopyranosyl moieties in a relative proportion of approximately 1:2:1:1. This polysaccharide showed optimum activation of macrophages as well as splenocytes and thymocytes at 10µg/mL. Structural investigation was carried out using sugar analysis, methylation analysis, periodate oxidation study, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as:
Assuntos
Adjuvantes Imunológicos/química , Adjuvantes Imunológicos/isolamento & purificação , Agaricales/química , Glucanos/química , Glucanos/isolamento & purificação , Lentinula/química , Adjuvantes Imunológicos/farmacologia , Animais , Sequência de Carboidratos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Glucanos/farmacologia , Macrófagos/citologia , Macrófagos/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Camundongos , Dados de Sequência Molecular , Baço/citologia , Timócitos/citologia , Timócitos/efeitos dos fármacosRESUMO
A water-soluble polysaccharide, isolated from the hot water extract of the fruiting bodies of an edible mushroom, Lentinus squarrosulus (Mont.) Singer was found to contain d-galactose, l-fucose, and d-glucose in a molar ratio of nearly 1:1:5. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR experiments ((1)H, (13)C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: This polysaccharide showed macrophage as well as splenocyte and thymocyte activation.
